Clinical evidence to demonstrate that simultaneous growth of epithelial and fibroblast cells is essential for deep wound healing

Shrivastava, Ravi

doi:10.1016/j.diabres.2010.12.021

Cited by 25 publications

(15 citation statements)

References 32 publications

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“…The great majority of the phenolic compounds of the chestnut honey in the study derived from condensed tannins. Tannins are phenolic polymers consisting of multiple anthocyanin-like molecules 37 . Phenolic compounds were also analyzed by exposure to acid hydrolysis in order to reveal total tannin or condensed flavonoids in the chestnut honey.…”

Section: Resultsmentioning

confidence: 99%

A comparative study of the antihyaluronidase, antiurease, antioxidant, antimicrobial and physicochemical properties of different unifloral degrees of chestnut (Castanea sativa Mill.) honeys

Kolaylı

Can

Yıldız

et al. 2016

Journal of Enzyme Inhibition and Medicinal Chemistry

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This study was planned to investigate some physicochemical and anti-inflammatory, antioxidant, antimicrobial properties of three different degrees of unifloral characters of chestnut honeys. Antihyaluronidase, antiurease and antimicrobial activities were evaluated as anti-inflammatory characteristics. Total phenolic contents, flavonoids, tannins, phenolic profiles, ferric-reducing antioxidant power (FRAP), scavenging activities of 2,2 0 -azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS + ) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals were evaluated as antioxidant properties. Color, optical rotation, conductivity, moisture, pH and ash content were evaluated as physicochemical parameters, and some sugars content, prolin, diastase, HMF and minerals (Na, K, Ca, P, Fe, Cu and Zn) were evaluated as chemical and biochemical parameters. All studied physicochemical and biological active properties were changed in line with the unifloral character of the chestnut honeys. A higher unifloral character was found associated with greater apitherapeutic capacity of the honey, as well as biological active compounds.

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Section: Resultsmentioning

confidence: 99%

A comparative study of the antihyaluronidase, antiurease, antioxidant, antimicrobial and physicochemical properties of different unifloral degrees of chestnut (Castanea sativa Mill.) honeys

Kolaylı

Can

Yıldız

et al. 2016

Journal of Enzyme Inhibition and Medicinal Chemistry

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“…Plant extracts rich phenolics compounds including catechin and epicatechin stimulated the growth of ephitelial and fibroblast cell as well as enhance the vessels formation in vitro [52,53], catechin was able to increase the viability of human umbilical vein endothelial cells (HUVEC), reducing apoptosis, but it contoled the angiogenesis in excess of VEGF [54].…”

Section: Discussionmentioning

confidence: 99%

Chemical profiling of Curatella americana Linn leaves by UPLC-HRMS and its wound healing activity in mice

et al. 2020

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Based on ethnopharmacological studies, a lot of plants, as well as its compounds, have been investigated for the potential use as wound healing agents. In Brazil, Curatella americana is traditionally used by local people to treat wounds, ulcers and inflammations. However, to the best of our knowledge, its traditional use in the treatment of wounds has not been validated by a scientific study. Here, some compounds, many of them flavonoids, were identified in the hydroethanolic extract from the leaves of C. americana (HECA) by LC-HRMS and LC-MS/MS. Besides that, solutions containing different concentrations of HECA and a gel produced with this extract were evaluated for its antimicrobial, coagulant and wound healing activities on an excision mouse wound model as well as its acute dermal safety. A total of thirteen compounds were identified in HECA, mainly quercetin, kaempferol and glucoside derivatives of both, besides catechin and epicatechin known as wound healing agents. The group treated with 1% of HECA exhibited highest wound healing activity and best rate of wound contraction confirmed by histopathology results. The present study provides scientific evidence of, this extract (HECA) possess remarkable wound healing activity, thereby, supporting the traditional use.

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“…In theory, any injury to the body's surface should heal rapidly as lesion repair simply necessitates filling the injured surface with identical cellular structures: epithelial cells, or fibroblast cells in case of deeper injuries for which it is more difficult to restore the normal physiological tissue structure in the injured cavity. 10 In chronic wounds, however, severe degradation of the ECM scaffolding essential to cell growth and tissue repair is the fundamental cause of the halted healing. The normal healing process begins immediately after the injury occurs, with the formation of a blood clot, followed by local inflammation, liberation of growth factors (particularly PDGF [platelet-derived growth factor], EGF [epithelial growth factor], and FGF [fibroblast growth factor]), activation of the body's physiological wound surface cleaning mechanism through the liberation of multiple MMPs to break down waste protein molecules, cell migration and proliferation in a clean environment, and finally tissue remodeling with normal cellular structure or with scar tissue.…”

Section: Discussionmentioning

confidence: 99%

“…Deep wound healing therefore requires simultaneous growth of fibroblast and epithelial cells. 9,10 In spite of the last decades' continuous research, there is currently no truly efficient cell growth-promoting treatment for chronic wounds. 11 Almost all existing treatments only partially provide the conditions necessary for cell growth.…”

Section: Introductionmentioning

confidence: 99%

A new generation of topical chronic wound treatments containing specific MMP inhibitors

Shrivastava¹,

Cucuat²,

Rousse³

et al. 2014

CWCMR

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Incidence of chronic wounds is constantly rising worldwide, but all currently available treatments are intended either to provide symptomatic relief or to assist cicatrization to some extent, but not to directly stimulate cellular growth. Physiologically, chronic wound healing simply requires cell growth to fill the injured cavity. To grow, our cells need to attach onto a cushion, called extracellular matrix (ECM), secreted by the mother cells and composed of multiple proteins. Recent scientific works prove that the concentration of certain matrix metalloproteinases (MMPs) is extremely high in all chronic wounds and, because of their proteolytic nature, some MMPs completely degrade the ECM, hindering cell attachment and cell growth. The aim of this study was to identify, neutralize, and eliminate these MMPs from the wound surface so as to design an effective treatment for chronic wounds. Methods: Acute and chronic models of human epithelial and fibroblast cells were prepared on a defined ECM cushion in vitro and MMPs were added in the culture medium to identify the MMPs causing ECM disintegration for each cell type. ECM-degrading MMPs were then incubated with selected procyanidin-rich plant extracts (PCDs) and cell growth was reanalyzed. Results: It was observed that: 1) multiple MMPs are involved in cellular matrix destruction; 2) ECM-destroying MMPs are specific with respect to cell type; and 3) specific PCDs may bind and neutralize selected MMPs. Conclusion: Topical application of specific plant PCDs to selectively neutralize ECMdestroying MMPs in acute and chronic wounds represents a novel approach for the treatment of superficial and deep skin wounds.

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Clinical evidence to demonstrate that simultaneous growth of epithelial and fibroblast cells is essential for deep wound healing

Cited by 25 publications

References 32 publications

A comparative study of the antihyaluronidase, antiurease, antioxidant, antimicrobial and physicochemical properties of different unifloral degrees of chestnut (Castanea sativa Mill.) honeys

A comparative study of the antihyaluronidase, antiurease, antioxidant, antimicrobial and physicochemical properties of different unifloral degrees of chestnut (Castanea sativa Mill.) honeys

Chemical profiling of Curatella americana Linn leaves by UPLC-HRMS and its wound healing activity in mice

A new generation of topical chronic wound treatments containing specific MMP inhibitors

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