Chemical profiling of Curatella americana Linn leaves by UPLC-HRMS and its wound healing activity in mice

Abstract

Based on ethnopharmacological studies, a lot of plants, as well as its compounds, have been investigated for the potential use as wound healing agents. In Brazil, Curatella americana is traditionally used by local people to treat wounds, ulcers and inflammations. However, to the best of our knowledge, its traditional use in the treatment of wounds has not been validated by a scientific study. Here, some compounds, many of them flavonoids, were identified in the hydroethanolic extract from the leaves of C. americana (HECA) by LC-HRMS and LC-MS/MS. Besides that, solutions containing different concentrations of HECA and a gel produced with this extract were evaluated for its antimicrobial, coagulant and wound healing activities on an excision mouse wound model as well as its acute dermal safety. A total of thirteen compounds were identified in HECA, mainly quercetin, kaempferol and glucoside derivatives of both, besides catechin and epicatechin known as wound healing agents. The group treated with 1% of HECA exhibited highest wound healing activity and best rate of wound contraction confirmed by histopathology results. The present study provides scientific evidence of, this extract (HECA) possess remarkable wound healing activity, thereby, supporting the traditional use.

A proline derivative-enriched methanol fraction from Sideroxylon obtusifolium leaves (MFSOL) stimulates human keratinocyte cells and exerts a healing effect in a burn wound model

“…These substances are generally absorbed well by the skin and modulate different stages of healing ( 26 ). Fujishima et al identified 13 compounds in a hydroethanolic extract from the leaves of C. americana , mainly quercetin, kaempferol, glucoside derivatives, catechin, and epicatechin, which could explain the excellent healing effect ( 27 ).…”

The wound healing effect of botanicals and pure natural substances used in in vivo models

“…exerted remarkable wound healing properties due to its active constituents. Leaves contain compounds known as wound-healing agents, mainly quercetin, kaempferol, glucosides, catechin, and epicatechin (Fujishima et al 2020).…”

Curatella americana uma espécie característica do Cerrado pouco conhecida: características da sistemática, fenologia, fitoquímica e fitomedicinal

“…Nos estudos de Fujishima et al (2020), os pesquisadores encontraram inúmeros compostos através de análises por cromatografia líquida de alta eficiência (CLAE) americana coletadas no estado do Mato grosso, Brasil. Os pesquisadores obtiveram concentração de inibição com CI50 de 6,0 µg mL -1 e atividade máxima de 96,5% (25 µg mL -1 ), quando comparados ao BHT com CI50 18,3 µg mL -1 , e máxima atividade de 93,7% (500 µg mL -1 ), e para o ácido ascórbico com CI50 de 1,8 µL mL -1 e máxima atividade de 92,3 (10 µg mL -1 ).…”