Clinical Correlates of a Lateral‐Flow Immunoassay Oral Risk Indicator

Nwhator, Solomon O.; Ayanbadejo, P O; Umeizudike, Kehinde Adesola; Opeodu, OI; Agbelusi, GA; Olamijulo, Joseph Ayodeji; Asani, MO; Sorsa, Timo; Babajide, B.S.; Opedun, D.O.

doi:10.1902/jop.2013.130116

Cited by 66 publications

(130 citation statements)

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“…The test has been independently and internationally successful validated in Africa, Europe and USA, and in all studies it found to excellently differentiate periodontal health and disease [71,73,74,75,76]. …”

Section: Development Of Point Of Care/chairside Test For Oral (Permentioning

confidence: 99%

Salivary Diagnostics—Point-of-Care diagnostics of MMP-8 in dentistry and medicine

et al. 2017

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Human saliva is an easily accessible biological fluid and contains a variety of disease-related biomarkers, which makes it a potential diagnostic medium. The clinical use of salivary/oral fluid biomarkers to identify oral and systemic conditions requires the development of non-invasive screening and diagnostic technologies, and is among the main goals of oral fluid researchers. The analysis of the disease-specific oral and systemic biomarkers in saliva and oral fluids (i.e., mouth-rinse, gingival crevicular fluid (GCF) and peri-implantitis sulcular fluid (PISF)) is demanding. Several factors influence their expression and release; these factors include the intracellular location, the molecular size and the flow characteristics of the biological fluid. The type of saliva/oral fluid utilized for the diagnostics affects the analysis. High sensitivity together with sophisticated methods and techniques are essential to get a useful outcome. We describe here a recently developed mouth-rinse that is practical, convenient and inexpensive, as well as PISF chair-side/point of care (PoC) lateral-flow active matrix metalloproteinase (aMMP-8) immunoassays to detect, predict and monitor the course and treatment of periodontitis and peri-implantitis.

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Section: Development Of Point Of Care/chairside Test For Oral (Permentioning

confidence: 99%

Salivary Diagnostics—Point-of-Care diagnostics of MMP-8 in dentistry and medicine

et al. 2017

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“…22,23 Activated matrix metalloproteinase-8 (aMMP-8) is already known as one of the most validated periodontitis biomarkers. 24,29,30,[36][37][38][39][40][41][42][43][44][45][46] Moreover, previously there have been found a moderate positive association/correlation between MMP-8/PGLYRP1 axis and TREM-1 levels among adults, 14 but to the best of authors' knowl-edge, this association hasn't been studied among adolescent subjects before. [24][25][26][27][28][29][30][31][32][33][34][35] This stage can be measured using a point-of-care (PoC) lateral flow collagenase-2 (aMMP-8) immunotest.…”

Section: Introductionmentioning

confidence: 99%

A point‐of‐care test of active matrix metalloproteinase‐8 predicts triggering receptor expressed on myeloid cells‐1 (TREM‐1) levels in saliva

Räisänen

Heikkinen

Esmaeili

et al. 2019

Journal of Periodontology

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Background This cross‐sectional study aims to investigate if a point‐of‐care (PoC) test of active matrix metalloproteinase‐8 (aMMP‐8) predicts levels of inflammation amplifier triggering receptor expressed on myeloid cells‐1 (TREM‐1) and its putative ligand the neutrophil peptidoglycan recognition protein 1 (PGLYRP1) in saliva. Methods Forty‐seven adolescents, aged 15 to 17 years, were tested with aMMP‐8 PoC test, which was followed by a full‐mouth clinical examination of the assessment of periodontal, mucosal, and oral health. TREM‐1 and PGLYRP1 levels were analyzed by ELISA. The immunofluorometric assay (IFMA) specific for aMMP‐8 was used as the reference method. Results Fourteen saliva samples out of a total of 47 showed positivity for aMMP‐8 PoC test. Both the TREM‐1 and the aMMP‐8 (IFMA) levels were significantly elevated among the aMMP‐8 PoC test positives compared with the PoC test negatives (P < 0.05). Moreover, aMMP‐8 levels assessed by IFMA showed a strong positive correlation with TREM‐1 levels in saliva (r = 0.777, P < 0.001). The number of sites with a probing depth of ≥4 mm was significantly lower among the adolescents that had a negative aMMP‐8 PoC test result, and TREM‐1 levels < 75 pg/mL (P < 0.05). In contrast, adolescents with a positive aMMP‐8 PoC test result (i.e., elevated aMMP‐8 levels) together with elevated TREM‐1 levels had a significantly higher number of periodontal pockets with ≥4 mm (P < 0.001). Conclusion The present study validated usability of aMMP‐8 PoC test for predicting “proinflammatory” salivary profile and periodontal health status in adolescents.

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“…Indeed, a quantitative POC activated MMP8 (aMMP8) oral fluid test has been repeatedly and independently validated in Finland, Germany, Nigeria, Turkey, the Netherlands and the United States to successfully screen susceptible sites and patients, differentiate active and inactive perio dontal sites, predict the future disease progression and monitor treatment response and mainten ance therapy [3][4][5][6][7][8] , with a diagnostic sensitivity and specificity of 76-83% and 96%, respectively, and a turnaround time of 5-7 minutes. Furthermore, the test can identify initial periodontitis in genetically predisposed adolescents 5 .…”

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confidence: 99%

“…Furthermore, the test can identify initial periodontitis in genetically predisposed adolescents 5 . Thus, the aMMP8 test is effective in both adolescent and adult populations 3,4 . The predictive value of the test lies in its ability to detect subclinical periodontitis before clinical or X-ray manifestations, as the test is positive (that is, aMMP8 level in oral fluids is elevated) ahead of active periodontal disease [9][10][11][12][13] .…”

mentioning

confidence: 99%

“…The test is, therefore, very suitable for monitoring disease progression and tailor ing preventive and therapeutic measures 14 . The aMMP8 test is inexpensive, easy to use (the results are automated and, therefore, independent of the practitioner's experience) and currently available for routine use by dental and medical professionals linking these disciplines [3][4][5]8,15 . Finally, we agree with the authors of the Primer that additional work, especially on the prognostic value of bio markers in periodontal and peri-implant diseases, is still required.…”

mentioning

confidence: 99%

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A quantitative point-of-care test for periodontal and dental peri-implant diseases

Sorsa

Gieselmann

Arweiler

et al. 2017

Nat Rev Dis Primers

142

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Clinical Correlates of a Lateral‐Flow Immunoassay Oral Risk Indicator

Abstract: Neutrophil collagenase-2 immunoassay had a high sensitivity for at least two sites with BOP and two sites with periodontal pockets but a lower relationship for single-site pockets and BOP.

Cited by 66 publications

References 29 publications

Salivary Diagnostics—Point-of-Care diagnostics of MMP-8 in dentistry and medicine

Salivary Diagnostics—Point-of-Care diagnostics of MMP-8 in dentistry and medicine

A point‐of‐care test of active matrix metalloproteinase‐8 predicts triggering receptor expressed on myeloid cells‐1 (TREM‐1) levels in saliva

A quantitative point-of-care test for periodontal and dental peri-implant diseases

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