Clinical associations between acetylcholine levels and cholinesterase activity in saliva and gingival crevicular fluid and periodontal diseases

Apatzidou, Danae Anastasia; Iskas, Achilleas; Konstantinidis, Antonis; Alghamdi, Abeer M.; Tumelty, Maria; Lappin, David F.; Nile, Christopher J.

doi:10.1111/jcpe.12989

Cited by 21 publications

(18 citation statements)

References 47 publications

(82 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Furthermore, non‐parametric correlation analysis indicated that baseline ACh concentration and AChE activity levels correlated with MBL recorded at 1 month. This is in agreement with a previous study which demonstrated that ACh levels and esterase activity in saliva and gingival crevicular fluid correlated with alveolar bone loss in chronic periodontitis (Apatzidou et al, ).…”

Section: Discussionsupporting

confidence: 94%

“…In this study, we report for the first time that levels of ACh, This is in agreement with a previous study which demonstrated that ACh levels and esterase activity in saliva and gingival crevicular fluid correlated with alveolar bone loss in chronic periodontitis (Apatzidou et al, 2018).…”

Section: Discussionsupporting

confidence: 93%

“…In this study, levels of IL-17A and IL-17E, as well as the (Zenobia & Hajishengallis, 2015). Gingival crevicular fluid and saliva concentrations of ACh have previously been found to significantly correlate with levels of IL-17A in patients with periodontal disease (Apatzidou et al, 2018).…”

Section: Indeed Osteoblasts Express Both Nicotinic (Nachrs) and Muscmentioning

confidence: 51%

“…IL‐17A has been demonstrated to have potent pro‐osteoclastogenic effects and be a key driver of alveolar bone loss in periodontal diseases (Zenobia & Hajishengallis, ). Gingival crevicular fluid and saliva concentrations of ACh have previously been found to significantly correlate with levels of IL‐17A in patients with periodontal disease (Apatzidou et al, ). Indeed, previous studies have suggested that there may be links between cholinergic systems and expression of IL‐17A in mucosal tissues.…”

Section: Discussionmentioning

confidence: 95%

“…AChE and BChE activity levels were determined using a modified version of the biochemical assay developed by Naik, Liu, and Saxena () as previously described (Apatzidou et al, ).…”

Section: Methodsmentioning

confidence: 99%

See 4 more Smart Citations

Cholinergic signalling mechanisms and early implant healing phases in healthy versus generalized aggressive periodontitis patients: A prospective, case–control study

Meriç

Buduneli

Kanmaz

et al. 2019

J Clinic Periodontology

Self Cite

View full text Add to dashboard Cite

Aims Periodontal diseases negatively affect implant osseointegration. Perturbations in non‐neuronal cholinergic signalling mechanisms are associated with periodontitis; however, their role in generalized aggressive periodontitis (GAgP) is unknown. The aim of this prospective case–control study was to determine the relationship between non‐neuronal cholinergic signalling mechanisms, secreted Ly‐6/uPAR‐related protein‐1 (SLURP‐1), interleukin‐17 (IL‐17) family cytokines and healing of dental implants in health and GAgP. Material and Methods Thirteen GAgP patients and seven periodontally healthy individuals (PH) were recruited. Peri‐implant crevicular fluid (PICF) was obtained at baseline and 1 month post‐placement. Acetylcholine (ACh) levels and cholinesterase activity were determined biochemically. SLURP‐1, IL‐17A and IL‐17E levels were determined by ELISA. Marginal bone loss (MBL) at 1 and 6 months post‐placement was determined radiographically. Results The concentration of ACh, cholinesterase activity and IL‐17A levels was elevated in PICF of patients with GAgP compared to PH individuals at baseline and 1 month post‐placement. The concentration of ACh and cholinesterase activity levels in PICF correlated with levels of IL‐17A and MBL around implants 1 month post‐placement in patients with GAgP. Conclusions Non‐neuronal cholinergic mechanisms may play a role in the aetiopathogenesis of GAgP and may directly or indirectly, through modulation of IL‐17A, influence early implant osseointegration and potential long‐term implant survival.

show abstract

Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 93%

Section: Indeed Osteoblasts Express Both Nicotinic (Nachrs) and Muscmentioning

confidence: 51%

Section: Discussionmentioning

confidence: 95%

“…AChE and BChE activity levels were determined using a modified version of the biochemical assay developed by Naik, Liu, and Saxena () as previously described (Apatzidou et al, ).…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations