Clinical and pathological effects of the dermonecrotic toxin of Bordetella bronchiseptica and Pasteurella multocida in specific-pathogen-free piglets.

Eliás, B; Boros, Gergely; Tran, Albert; Tuboly, S; Gergely, P; Papp, László; Vetro, I. Barna; Rafai, P.; Molnár, E.

doi:10.1292/jvms1939.52.677

Cited by 19 publications

(7 citation statements)

References 13 publications

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“…After incubation with (-) or without (0) DNT (5 ng/ml) for the indicated periods, the cells were labeled with [methyl-3H]thymidine for 2 h. Each point indicates the mean + standard deviation of three determinations. 4. Effect of DNT dose on DNA synthesis in the serumstarved MC3T3-E1 cells.…”

Section: 3%)mentioning

confidence: 99%

“…Bordetella bronchiseptica dermonecrotic toxin (DNT) is considered to be a vinilence factor causing turbinate atrophy in swine atrophic rhinitis (1,3,4,8,14). Several reports suggested that DNT caused degenerative effects on bone tissue (4,8,14). However, the exact role of the toxin in production of turbinate atrophy has not been clarified because of the lack of a highly purified toxin preparation and an appropriate in vitro model for analysis of its action on bone tissue.…”

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confidence: 99%

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Stimulation of DNA synthesis in osteoblast-like MC3T3-E1 cells by Bordetella bronchiseptica dermonecrotic toxin

1993

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We investigated the effects of BordeteUa bronchiseptica dermonecrotic toxin on DNA synthesis in MC3T3-E1 cells. The rate of [methyl-3H]thymidine incorporation increased in the toxin-treated cells more than 24 h after addition of the toxin under the serum-starved conditions. This effect was dependent on the toxin concentration ranging from 0.3 to 3 ng/ml and was eliminated by aphidicolin and hydroxyurea, inhibitors for DNA replication. In the toxin-treated culture, the number of cells did not increase but polynucleated cells appeared and their number increased to ca. 50% of the total number of cells 6 days after the toxin addition. From these results, we concluded that the toxin stimulates DNA replication in MC3T3-E1 cells without cell proliferation.

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Section: 3%)mentioning

confidence: 99%

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confidence: 99%

Stimulation of DNA synthesis in osteoblast-like MC3T3-E1 cells by Bordetella bronchiseptica dermonecrotic toxin

1993

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“…Little is known about the mechanism by which some bacteria cause rapid necrosis of the subcutaneous tissues. P. multocida can produce a dermonecrotic toxin which is unique and the primary causative agent of pro gressive atrophic rhinitis of pigs [8]. However, it is still unclear whether this toxin plays an important role in the pathogenesis of human necrotizing fasciitis.…”

Section: Discussionmentioning

confidence: 99%

Necrotizing Fasciitis due to <i>Pasteurella multocida</i> Infection

Hamamoto¹,

Soejima²,

Ogasawara³

et al. 1995

Dermatology

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Necrotizing fasciitis is a potentially fatal clinical disease caused by infection with various bacteria in addition to streptococci, which are common causative agents. We report on a rare case of this disease in association with Pasteurella multocida infection. A 58-year-old man had systemic features of shock after a 15-hour history of a painful swelling on the right lower leg. The swelling led to skin blistering and necrosis from which P. multocida was isolated. Those lesions progressed rapidly. The patient also had a history of chronic liver injury as described in previous reports.

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“…Early descriptions of assays used to monitor the activity of the toxin preparations included induction of turbinate atrophy after intranasal exposure in piglets [14,20,[31][32][33][34][35][36][37]; induction of atrophic rhinitis and other organ lesions after intramuscular, intraperitoneal, or intravenous injection [31,[36][37][38][39][40][41]; induction of atrophic rhinitis and pneumonia in rabbits [34,42]; guinea pig or rat dermonecrotic lesion assays [34,[43][44][45]; BALB/c mouse lethality assay [18,34,44,46]; and cell culture assays of cytopathicity [42,[47][48][49]. Results from these studies demonstrated that purified Pasteurella multocida toxin (PMT) alone could induce turbinate atrophy and other symptoms of disease, as well as lethality, dermonecrotic, cytopathic, mitogenic, and other biological effects.…”

Section: Role Of Pmt In Pathogenesismentioning

confidence: 99%