Clinical and Diagnostic Significance of Aspartate Aminotransferase Isoenzymes in Sera of Patients with Liver Diseases

Abstract

Summary: Serum aspartate aminotransferase isoenzymes were measured in 123 hospital patients with various liver diseases, using a new and simple immunochemical method.Our findings show the usefulness of this determination in estimating the severity of hepatic damage, especially if accompanied by the measurement of other mitochondrial enzymes/During hepatic damage, the cytoplasmic isoenzyme is found in greater quantities than the mitochondrial isoenzyme, but the level of the latter increases to a greater extent in acute liver diseases. Moreover, the values of mitochondrial aspartate aminotransferase activity and the ratio of mitochondrial to total aspartate aminotransferase in alcoholic hepatitis are higher than expected if so-called hepatic enzymes commonly measured in serum are considered. These results indicate that there is significant mitochondrial damage in alcoholic hepatitis. Klinische und diagnostische Bedeutung von

Laboratory Markers of Alcoholism

“…Follow-up of 76 patients after 1 week of abstinence showed that the mito chondrial AST was falling rapidly towards normal. Similar results have been obtained by Panteghini et al [27], In summary, the measurement of mitochondrial AST-to-total AST ratios appears to be very promising as a specific and sensitive laboratory marker of alcoholism, but these results need to be con firmed by other groups.…”

Liver function changes following the introduction of a gluten-free diet in patients with celiac disease

“…This may be explained by the fact that ALT is historically considered hepatocyte-specific, and thus may reflect the direct effects of gluten sensitization on hepatocytes [ 23 , 24 ]. Furthermore, ALT is a cytosolic enzyme, in contrast to AST, which occurs in both the mitochondria and cytoplasm [ 23 , 25 ]. GGT comes mainly from cholangiocytes, which seem to be less affected by gluten sensitization than hepatocytes, but could be affected by the concomitant biliary disorders rarely seen in association with CD [ 6 , 11 , 21 ].…”

Estimation of the enzymes LDH, GOT and GPT in plasma of grey mullet Mugil auratus and their significance in liver intoxication

“…Detection methods for these enzymes are simple, rapid and, what is important in fish physiology studies, can be carried out with small blood samples. The determination of the basic physicochemical characteristics and electrophoretic fractionation of the isozyme forms provides the opportunity to identify their origin and also permits estimation of the seventy of injury at the tissue/cellular level, as was illustrated by Panteghini et al (1984).…”