These data indicate that, in man, neutrophils do accumulate in the myocardium during early reperfusion. However, they are not activated when oxidative stress occurs. It is unlikely that the neutrophil localisation in the heart has pathological significance in the production of oxygen free radicals during early reperfusion. Free radical accumulation in the coronary vessels may contribute to disorders of coronary flow associated with reperfusion.

Summary: Serum aspartate aminotransferase isoenzymes were measured in 123 hospital patients with various liver diseases, using a new and simple immunochemical method.Our findings show the usefulness of this determination in estimating the severity of hepatic damage, especially if accompanied by the measurement of other mitochondrial enzymes/During hepatic damage, the cytoplasmic isoenzyme is found in greater quantities than the mitochondrial isoenzyme, but the level of the latter increases to a greater extent in acute liver diseases. Moreover, the values of mitochondrial aspartate aminotransferase activity and the ratio of mitochondrial to total aspartate aminotransferase in alcoholic hepatitis are higher than expected if so-called hepatic enzymes commonly measured in serum are considered. These results indicate that there is significant mitochondrial damage in alcoholic hepatitis. Klinische und diagnostische Bedeutung von

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A simultaneous two-site immunoenzymometric assay for creatine kinase MB determination (Hybritech Tandem-Ε CK-MB) using monoclonal antibodies was evaluated and compared with cellulose acetate electrophoresis using fluorometric scanning densitometry.The assay has satisfactory precision (between-day analysis gives a coefficient of Variation between 2.1 and 9.4%) and is not susceptible to interference by concentrations of creatine kinase MM up to 5000 μg/l (3400 U/l) and creatine kinase BB up to 1000 μg/l (1085 U/l). The upper limit of MB isoenzyme concentration in 250 apparently healthy people was 5.5 μg/l. Comparison between the immunoenzymometric assay (y) and electrophoresis (x) yielded the following linear regression equation: y = 0.37 χ -h 1.9, with a correlation coefficient of 0.828. The characteristics of the temporal kinetics of MB isoenzyme, calculated by two methods, in 49 patients with acute myocardial infarction, were nearly identical in terms of the rate of creatine kinase MB release and the time at which the peak value is obtained, but not in terms of the rate of elimination of the isoenzyme. The fractional disappearance rate of MB isoenzyme from the circulation was significantly higher if calculated with Tandem-Ε results rather than with electrophoresis results (-0.035 vs -0.028, p < 0.001). Whereas in the first day after infarction immunoenzymometric assay and electrophoresis had the same clinical sensitivity for identifying patients with acute myocardial infarction, in specimens collected more than 24 hours after the onset of the ehest pain, the clinical sensitivity of the immunoenzymometric method was lower. Our results show that it is stiU premature to draw definitive clinical conclusions from the iinmunoassay results. Evaluierung eines k uflichen immunenzymometrischen Testbestecks zur Bestimmung von Kreatinkinase MBIsoenzym mit monoklonalen Antik rpernZusammenfassung: Ein gleichzeitig auf zwei Teilkomponenten gerichteter enzymimmunometrischer Assay zur Bestimmung von Kreatinkinase MB mit monoklonalen Antik rpern (Hybritech Tandem-Ε CK-MB) wurde ev luiert und mit der CeUuloseacetat-Elektrophorese mit fluorimetrischer Scanning-Densitometrie verglichen. Die Bestimmung hat eine gen gende Pr zision (VK von Tag zu Tag 2,1 -9,4%) und wird durch Konzentrationen von Kreatinkinase MM bis zu 5000 μ$/1 (3400 U/l) und von Kreatinkinase BB bis zu 1000 μg/l (1085 U/l) nicht gest rt. Die obere Grenze der Konzentration von Kreatinkinase MB bei 250 offenbar Gesunden war 5,5 μ §/1. Der Vergleich zwischen enzymometrischem Assay (y) und Elektrophorese (x) ergab die folgende Regressionsgleichung: y == 0,37 x + 1,9 mit einem Korrelationskoeffizienten von 0,828. Die Characteristika des zeitlichen Verlaufs der Konzentration von Isoenzym MB wurden bei 49 Patienten mit akutem Herzinfarkt nach zwei Methoden ermittelt. Sie waren bez glich der Freisetzungsrate von Kreatinkinase MB und der Zeit, zu der der Spitzenwert erhalten wird, nahezu identisch, nicht jedoch bez glich der Eliminationsrate des Isoenzyms. Die relative Schwu...

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