Estimation of the enzymes LDH, GOT and GPT in plasma of grey mullet Mugil auratus and their significance in liver intoxication

Krajnović-Ozretić, Mirjana; Ozretić, Bartolo

doi:10.3354/dao003187

Cited by 32 publications

(18 citation statements)

References 15 publications

(20 reference statements)

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“…These enzymes are found in small concentrations in plasma, derived probably from the regular physiological shedding of cells (Schmidt and Schmidt 1974). Therefore, any detectable increase of their activity in plasma can be a reliable indicator of not only changed metabolic functions but also of structural damage at tissue level, so the interpretation of results from these enzymes should be done carefully (Krajnovic-Ozretic and Ozretic 1987). In the present trial we observed an increasing value of GPT and GOT in response to the increasing values of AFB1 in the diets, suggesting tissue damage.…”

Section: Discussionmentioning

confidence: 43%

Response of yellow catfish (Pelteobagrus fulvidraco) to different dietary concentrations of aflatoxin B1 and evaluation of an aflatoxin binder in offsetting its negative effects

Wang

et al. 2016

Cienc. Mar.

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Response of yellow catfish (Pelteobagrus fulvidraco) to different dietary concentrations of aflatoxin B1 and evaluation of an aflatoxin binder in offsetting its negative effectsRespuesta del bagre amarillo (Pelteobagrus fulvidraco) a diferentes concentraciones dietéticas de aflatoxina B1 y la evaluación de una bentonita para contrarrestar sus efectos negativos ABSTRACT. Aflatoxin B1 (AFB1), produced by Aspergillus flavus and Aspergillus parasiticus, is one of the major mycotoxins to affect animal health. AFB1 contamination of aquafeed is widespread, especially in countries with humid, tropical climates. The most applied method for protecting animals against aflatoxicosis is the use of clay minerals. Growth performance, immunosuppressive effects (bactericidal activity, lysozyme activity, albumin/globulin ratio, alternative complement pathway activity, and protein content), and pathology parameters were used to evaluate the response of yellow catfish (Pelteobagrus fulvidraco) to increasing concentrations of AFB1 and test the protective effect of dietary supplementation with a bentonite (dioctahedral montmorillonite) based AFB1 binder. Triplicate groups of yellow catfish with an average weight of 2.0 ± 0.1 g were fed diets containing 0, 200, 500, and 1,000 g/kg of AFB1 alone, or diets containing 0, 200, 500, and 1,000 g/kg of AFB1 along with 2 g/kg AFB1 binder, for 12 weeks. Results showed that diets containing increasing amounts of AFB1 had a significantly lower (P = 0.002) survival rate. There was a statistical significant reduction in weight gain, final body weight, and specific growth rate, and an increase in feed conversion ratio (FCR) influenced by the levels of AFB1 in the diet (P < 0.001 for all parameters), as well as increasing protection due to the presence of the binder (P = 0.046, P = 0.014, P = 0.038, and P = 0.485, respectively). The immunosuppressive nature of AFB1 in yellow catfish diets was confirmed through observation of lower bactericidal activity (P = 0.001), lower lysozyme activity (P = 0.006), reduced total protein (P = 0.002), and enhanced albumin/globulin ratio (P = 0.004). Fish fed diets contaminated with AFB1 and supplemented with the AFB1 binder showed better improvement in FCR (P = 0.019). These results indicate that AFB1 has a negative impact on yellow catfish growth and survival rate. The AFB1 binder protected fish from the toxic effects of AFB1. RESUMEN.La aflatoxina B1 (AFB1), producida por Aspergillus flavus y Aspergillus parasiticus, es una de las principales micotoxinas que afectan la salud animal. La contaminación de alimentos acuícolas con AFB1 es común, especialmente en países con climas húmedos y tropicales. El método más usado para proteger a los animales contra la aflatoxicosis es el uso de arcillas. Para evaluar la respuesta del bagre amarillo (Pelteobagrus fulvidraco) a concentraciones crecientes de AFB1 y evaluar el efecto protector de una bentonita (montmorillonita dioctaédrica), se analizaron el crecimiento, efectos inmunosupresores (actividad bactericida, relación a...

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Section: Discussionmentioning

confidence: 43%

Response of yellow catfish (Pelteobagrus fulvidraco) to different dietary concentrations of aflatoxin B1 and evaluation of an aflatoxin binder in offsetting its negative effects

Wang

et al. 2016

Cienc. Mar.

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“…A019-2) produced by Jiancheng Bioengineering Institute (Nanjing, China). Activities of plasma aspartate aminotransferase (GOT) and alanine aminotransferase (GPT) were estimated according to the methods described by Krajnovi c-Ozreti c and Ozreti c [32].…”

Section: Plasma Stress Parameters and Liver Damage Diagnosismentioning

confidence: 99%

Dietary carbohydrate/lipid ratios affect stress, oxidative status and non-specific immune responses of fingerling blunt snout bream, Megalobrama amblycephala

Liu

et al. 2012

Fish & Shellfish Immunology

118

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Section: Introductionmentioning

confidence: 99%

“…There is mounting evidence that serum enzymes [alanine aminotransferase (ALAT, formerly SGPT); aspartate aminotransferase (ASAT, formerly SGOT); lactate dehydrogenase (LDH); creatine kinase (CK)] possess special diagnostic magnitudes to several pollutant categories (Krajnoviñ-Ozretiñ and Ozretiñ 1987;NemcsoÂ k et al 1987;Adham et al 1997). Additionally, the relative RNA and DNA content is used as an integrative indicator of contaminant stress and as a sensitive and rapid indicator for assessing the eects of contaminants on ®sh growth (Haines 1973; Barron and Adelman 1984;Rodreguez-Ariza et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Sublethal effects of aquatic pollution in Lake Maryut on the African sharptooth catfish, Clarias gariepinus (Burchell, 1822)

Adham

2002

J Appl Ichthyol

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Some aspects of the physiology and biochemistry of the African sharptooth catfish, Clarias gariepinus (Burchell, 1822), were studied along with an array of physicochemical characteristics of the water in Lake Maryût, Egypt. Data were compared to those of a reference fish hatchery. At least 11 of the conventional water pollutants (Cd, Cu, Fe, Pb, Mn, Hg, Ni, Zn, turbidity, chemical oxygen demand and ammonia nitrogen) were elevated in the most polluted main basin of the lake. In turn, serologic analysis of the indigenous catfish C. gariepinus pointed at functional impairment of the liver, heart and kidney as reflected by the elevated activities of alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, creatine kinase, cholinesterase, glucose and creatinine. Reduced nucleic acid measurements [the ratio RNA/DNA and the relative RNA content (r)] indicated diminished protein synthesis and impaired growth in polluted fish. In some instances, glucose and nucleic acid measures were elevated in favour of fish from moderately polluted basins of the lake rather than in reference fish. Fish seemed to profit from the typical geographical habitat in the lake, regardless of the virtual contamination there, versus reference fish that seemed to suffer congestion stress and food competition in the confined and overcrowded commercial pond.

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Estimation of the enzymes LDH, GOT and GPT in plasma of grey mullet Mugil auratus and their significance in liver intoxication

Cited by 32 publications

References 15 publications

Response of yellow catfish (Pelteobagrus fulvidraco) to different dietary concentrations of aflatoxin B1 and evaluation of an aflatoxin binder in offsetting its negative effects

Response of yellow catfish (Pelteobagrus fulvidraco) to different dietary concentrations of aflatoxin B1 and evaluation of an aflatoxin binder in offsetting its negative effects

Dietary carbohydrate/lipid ratios affect stress, oxidative status and non-specific immune responses of fingerling blunt snout bream, Megalobrama amblycephala

Sublethal effects of aquatic pollution in Lake Maryut on the African sharptooth catfish, Clarias gariepinus (Burchell, 1822)

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