Clinical and aetiological study of adenoviral conjunctivitis, with special reference to adenovirus types 4 and 19 infections.

Aoki, Koki; Katô, Masahiko; Ohtsuka, H; Ishii, K.; Nakazono, Naoki; Sawada, H

doi:10.1136/bjo.66.12.776

Cited by 46 publications

(32 citation statements)

References 17 publications

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“…The adenovirus types that are most commonly associated with respiratory infections are HAdV-3, 7, and 21 of species B; HAdV-1, 2, 5, and 6 of species C; and, HAdV-4 of species E (21,22). HAdV-E4, the only member of species E, often causes conjunctivitis and respiratory infections (2,23,24).…”

Section: Discussionmentioning

confidence: 99%

Detection and Molecular Typing of Human Adenoviruses Associated with Respiratory Illnesses in Kerala

2016

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SUMMARY: Adenoviruses are responsible for approximately 5-10z of acute respiratory infections globally. However, there are a limited number of reports on the types of circulating respiratory human adenoviruses (HAdV) in India. We detected HAdV in the post-mortem specimens of a young child who died as a result of an acute febrile illness. To retrospectively investigate the circulating adenovirus types in the Alappuzha region, samples (n ＝ 235) collected from patients with influenza-like illnesses who participated in the influenza surveillance program were screened for HAdV. Fourteen samples were identified as positive for adenovirus by PCR analysis. Adenovirus was isolated from 3 of the 14 PCRpositive samples cultured using HEK-293 cell lines. The viral strains isolated in the study were from children between 6 and 10 years of age. The isolates were identified as adenovirus species C and E. Sequencing analysis of the fiber gene and a BLAST search revealed that 2 of the isolates were type HAdV-C2, and the third isolate was a HAdV-E4. A fiber gene sequence-based phylogenetic tree showed that the HAdV-E4 isolate was similar to the Japanese HAdV-E4 strain, whereas the HAdV-C2 isolates formed a distinct cluster. Respiratory infections due to HAdV-E4 are generally observed in adults; this study is the first to demonstrate the involvement of the HAdV-E4 strain in respiratory illnesses in children.

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Section: Discussionmentioning

confidence: 99%

Detection and Molecular Typing of Human Adenoviruses Associated with Respiratory Illnesses in Kerala

2016

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“…Conjunctivitis due to AdV is caused mainly by HAdV-3 (in HAdV-B), -4 (in HAdV-E), -8, -19, and -37 (all in HAdV-D). Among these, HAdV-8, -19, and -37 cause more-severe epidemic keratoconjunctivitis (EKC) than the others (2)(3)(4). Serotyping of HAdVs is generally performed by virus isolation followed by a neutralization test (NT) and hemagglutination inhibition test (HI) using type-specific antiserum (29).…”

Section: We Propose That This Virus Is a New Hexon-chimeric Intermedimentioning

confidence: 99%

Epidemic Keratoconjunctivitis Due to the Novel Hexon-Chimeric-Intermediate 22,37/H8 Human Adenovirus

et al. 2008

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In a 2-month period in 2003, we encountered an outbreak of epidemic keratoconjunctivitis (EKC) in Japan. We detected 67 human adenoviruses (HAdVs) by PCR from eye swabs of patients with EKC at five eye clinics in different parts of Japan. Forty-one of the 67 HAdV DNAs from the swabs were identified as HAdV-37 by phylogenetic analysis using a partial hexon gene sequence. When the restriction patterns of these viral genomes were compared with that of the HAdV-37 prototype strain, one isolate showed a never-before-seen restriction pattern. Within 1 year, we encountered three more EKC cases caused by a genetically identical virus: two nosocomial infections at two different university hospitals and a sporadic infection at an eye clinic. We determined the nucleotide sequences of the full-length hexon and fiber genes of these isolates and compared them to those of the 51 prototype strains. Surprisingly, the sequence of the hexon ( determinant) loop-1 and -2 regions showed the highest nucleotide identity with HAdV-22, a rare EKC isolate. However, the nucleotide sequence of the fiber gene was identical to that of the HAdV-8 prototype strain. 22 We propose that this virus is a new hexon-chimeric intermediate HAdV-22,37/H8, and may be an etiological agent of EKC.

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“…Adenoviral conjunctivitis is caused primarily by HAdV-3 (of the HAdV-B species), HAdV-4 (of HAdV-E), and HAdV-8, HAdV-19, and HAdV-37 (all of HAdV-D). Among these, HAdV-8, HAdV-19, and HAdV-37 cause more severe EKC than the others (3,4,7). The more serious outcomes occur in all age groups and can trigger highly contagious nosocomial infections (3,7,20,26,38).…”

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Novel Human Adenovirus Causing Nosocomial Epidemic Keratoconjunctivitis

et al. 2008

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In 2000, we encountered cases of nosocomial infections with epidemic keratoconjunctivitis (EKC) at a university hospital in Kobe, in the western part of Japan. Two human adenovirus (HAdV) strains, Kobe-H and Kobe-S, were isolated from patients with nosocomial EKC infection. They were untypeable by existing neutralizing antisera; however, the isolate was neutralized with homologous antisera. We then encountered several cases of EKC due to nosocomial infections in eye clinics in different parts of Japan. A total of 80 HAdVs were isolated from patients with EKC at eight different hospitals. The partial hexon gene sequences of the isolates were determined and compared to those of the prototype strains of 51 serotypes. All isolates had identical partial hexon nucleotide sequences. Phylogenetic analysis classified these isolates into species of HAdV-D. The isolates showed 93.9 to 96.7% nucleotide identity with HAdV-D prototype strains, while all 32 HAdV-D prototype strains ranged from 93.2 to 99.2% identity. The sequences of the loop 2 and fiber knob regions from the representative strain, Kobe-H, were dissimilar in all prototype strains of 51 serotypes. We believe that this virus is a novel serotype of HAdV that causes EKC.

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Clinical and aetiological study of adenoviral conjunctivitis, with special reference to adenovirus types 4 and 19 infections.

Cited by 46 publications

References 17 publications

Detection and Molecular Typing of Human Adenoviruses Associated with Respiratory Illnesses in Kerala

Detection and Molecular Typing of Human Adenoviruses Associated with Respiratory Illnesses in Kerala

Epidemic Keratoconjunctivitis Due to the Novel Hexon-Chimeric-Intermediate 22,37/H8 Human Adenovirus

Novel Human Adenovirus Causing Nosocomial Epidemic Keratoconjunctivitis

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