Click chemistry: A route to designing and preparing pseudo-biospecific immunoadsorbent for IgG adsorption

Contribution of the “Click Chemistry” Toolbox for the Design, Synthesis, and Resulting Applications of Innovative and Efficient Separative Supports: Time for Assessment

“…Suksrichavalit et al and Hu et al notably reported on the functionalization of agarose beads with azide groups. [53,54] This methodology entails the derivatization of surface hydroxyl groups of a commercially available agarose gel with epichlorhydrin reagent in aqueous alkaline conditions and the successive substitution of the as-obtained epoxide functions by sodium azide (Figure 3e). Similarly, The functionalization of amine-bearing agarose gels with either azide or alkyne clickable chemical groups was realized by Punna et al [55] This strategy involves the coupling of the agarose amine groups with p-nitrophenyl 5-azidopentanoate or p-nitrophenyl pent-4-ynoate (Figure 3e).…”

“…Histidine: L-Histidine has notably been anchored at the surface of sepharose gel via the CuAAC strategy. [54] The sepharose gel was first prepared by reaction with epichlorohydrin to afford the corresponding epoxide functionalized material that underwent a nucleophilic substitution in presence of sodium azide. In parallel, L-histidine was suitably functionalized with the complementary alkyne function.…”

Is click chemistry attractive for separation sciences?

“…Agarose gels are very popular in affinity chromatography mainly due to their inexpensive and biocompatible nature . Epoxy and amine functionalized agarose beads were used to prepare azide‐functionalized beads. Oriented immobilization of alkyne‐biotine and peptides derivatives was conveniently and easily realized on these clickable agarose supports ).…”

“…Peptides that contain primary amide, carboxylic acid, thiol, and alcohol functional groups would otherwise complicate the strategy of immobilization. In an effort to increase the significance of clickable agarose, Hu et al have prepared a pseudo‐biospecific material with less expensive clickable ligands than standard protein ligands (proteins A, G, L, and anti‐IgG ligands) for the development of safe, efficient, and affordable immunosorbents for IgG adsorption. l ‐Histidine has been reported to show particular ability in separating IgG from human plasma or serum.…”