Is click chemistry attractive for separation sciences?

Abstract

Trends in LC focus on dedicated separation developments spanning different fields of applications ranging from sample preparation to miniaturization. Chromatographic performances result from the porous media, its implantation inside the "column," and its surface functionalization. Because molecular interactions govern chromatographic phenomena, surface functionalization is still a hot research topic. Besides standard approaches for surface functionalization, the use of new surface chemistry reactions opens new perspectives. Click chemistry belongs to this new class of chemical reactions, characterized by its specificity, compatibility with aqueous media, and high reaction yields. In this frame, we review the use of click chemistry reactions in chromatographic sciences. In a first part, we present click chemistry with a specific focus on its implementation in stationary phases. The use of these new clicked materials is detailed and discussed with respect to the chromatographic mode.

One‐pot preparation of an organic polymer monolith by thiol‐ene click chemistry for capillary electrochromatography

“…Click chemistry is an emerging field, which is widely used in separation science . In particular, the thiol‐ene click polymerization has attracted increasing attention in the preparation of separating materials because it has high selectivity, requires mild synthesis conditions, and provides high reaction efficiency . Recently, monoliths prepared by thiol‐ene click chemistry have become popular and showed a continuous porous structure and good separation performance .…”

One‐pot synthesis of a new high vinyl content hybrid silica monolith dedicated to nanoliquid chromatography

“…These hybrid materials are also chemically reactive and can serve as “generic” platform for further “specific” modifications. As examples, bromination , hydroboration followed by alcoholysis or epoxidation, Grubbs metathesis, thiol‐ene click reactions , oxidation by potassium permanganate , hydrosulfite addition to the carbon–carbon double bond have been reported to tailor the surface chemistry of pendant vinyl groups on silica materials (postmodification), thus offering a large variety of surface chemistries. As an alternative to their post‐modification, a recent “one‐pot” approach has been proposed, by adding organic reactive monomers to the sol–gel mixture.…”

Enantioseparation tuned by solvent polarity on a β‐cyclodextrin clicked chiral stationary phase

“…Click chemistry was successfully introduced into the preparation of CSPs for chromatographic enantioseparations . Ng's group reported the “click” preparation of β‐CD CSPs for enantioseparation of over 40 pairs of enantiomers and a library of neutral and basic drugs in HPLC .…”