Contribution of the “Click Chemistry” Toolbox for the Design, Synthesis, and Resulting Applications of Innovative and Efficient Separative Supports: Time for Assessment

Abstract

The last two decades have seen the rapid expansion of click chemistry methodology in various domains closely related to organic chemistry. It has notably been widely developed in the area of surface chemistry, mainly because of the high-yielding character of reactions of the "click" type. Especially, this powerful chemical reaction toolbox has been adapted to the preparation of stationary phases from the corresponding chromatographic supports. A plethora of selectors can thus be immobilized on either organic, inorganic, or hybrid stationary phases that can be used in different chromatographic modes. This review first highlights the few different chemical ligation strategies of the "click" type that are up to now mainly devoted to the development of functionalized supports for separation sciences. Then, it gives in a second part an up-to-date survey of the different studies dedicated to the preparation of click chemistry-based chromatographic supports while highlighting the powerful and versatile character of the "click" ligation strategy for the design, synthesis, and developments of more and more complex systems that can find promising applications in the area of analytical sciences, in domains as varied as enantioselective separation, glycomics, proteomics, genomics, metabolomics, etc.

Click chemistry: A fascinating, Nobel-winning method for the improvement of biological activity

“…Researchers in the field of materials, polymers, and even biotechnology are in an ongoing pace of research on binding strategies that can be effectively used in the presence of a wide range of different functional groups encountered. To develop successful binding strategies, basic requirements such as high selectivity, tolerance against other functional groups, compatibility for water and other protic solvents, and most importantly, good quantitative yield must be provided [1][2][3] . Materials scientists and those working in the field of biotechnology have tended to advanced synthetic organic concepts day by day in order to progress in this direction and reach the goal.…”

Preparation of Protein A Membrane Adsorbers Using Strain-Promoted, Copper-Free Dibenzocyclooctyne (DBCO)-Azide Click Chemistry

“…Since then, it has been utilized widely in the fields of chemical biology for many applications, including preparation of antibody-drug conjugates and vaccines; imaging and profiling of all classes of biomolecules including but not limited to proteins, glycans, lipids, metabolites; immobilization of biomolecules onto stationary supports; and the development of new classes of biorthogonal chemistry like tetrazine ligation chemistry [2,3]. While Cu(I) alkyne-azide chemistry has been used to prepare chromatography media [4][5][6][7], there have been no instances in the literature where it has been used specifically for preparation of Protein A chromatography media.…”

Metallic Nanoparticles Adsorbed at the Pore Surface of Polymers with Various Porous Morphologies: Toward Hybrid Materials Meant for Heterogeneous Supported Catalysis

“…They can be produced through low-cost chemical processes and can be functionalized in a facile and straightforward fashion, either through post-polymerization chemical modification or by suitably selecting functional monomers of interest. It is also good to notice that some of these functionalizations can be achieved in mild experimental conditions, such as by using “click” chemistry modification strategies [ 23 ]. Moreover, a variety of different synthetic strategies is already well-known to produce such porous polymeric supports [ 24 ].…”