Clastogenic effects of food additive citric acid in human peripheral lymphocytes

Yılmaz, Serkan; Ünal, Fatma; Yüzbaşıoğlu, Deniz; Aksoy, Hüseyin

doi:10.1007/s10616-008-9137-0

Cited by 40 publications

(25 citation statements)

References 36 publications

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“…Single cell gel electrophoresis (SCGE) or ''Comet'' assay is used to determine the potential genotoxicity (Rencuzogulları et al, 2004;Yılmaz et al, 2008). It is a marker of the early biological effects of carcinogen exposure (Liou et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

UV radiation-induced oxidative stress and DNA damage on Mediterranean flour moth, Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) larvae

Güven

Pandır

Baş

2015

Turkish Journal of Entomology

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SummaryExposure to ultraviolet (UV) radiation is hazardous for all organisms. UV shows numerous effects on insects. In this study, mortality effect of UV on Ephestia kuehniella Zeller, 1879 (Lepidoptera: Pyralidae) larvae was studied. The larvae were exposed to UV radiation (254 and 365 nm) at different time periods (15, 30, 45 and 60 min). After exposure, mortality rate, comet assay studies, antioxidant enzyme activities (SOD, CAT, GST and GPx), and MDA levels were investigated on the larvae. The mortality rate of E. kuehniella larvae increased with increasing exposure times. It was determined that 254 nm was more effective than 365 nm compared to the effects of short (254 nm) and long-wave (365 nm) radiations on mortality rate of E. kuehniella larvae. Likewise mortality rate, 254 nm had more potency than 365 nm on antioxidant enzyme activities and levels of MDA. According to the comet assay results, the tail DNA% and comet tail length significantly increased in all exposure times at 254 nm, but these changes were seen only 45 min and 60 min at 365 nm of UV radiation. Therefore, tail DNA% and tail lenghts of 254 nm radiation could be greater than the tail lenghts of 365 nm UV radiation. As a result, the UV radiation could be effectively as a safe pest control method and as an alternative to environmentally hazardous chemical pesticides. Key words: Ephestia kuehniella, UV radiation, mortality, antioxidant enzymes, comet assay ÖzetUltraviyole (UV) radyasyona maruz kalmak tüm canlılar için tehlikelidir. UV böcekler üzerinde birçok etki göstermektedir. Bu çalışmada, Ephestia kuehniella 1879 (Lepidoptera: Pyralidae) larvaları üzerine UV'nin öldürücü etkisi çalışıldı. Larvalar UV radyasyonuna (254 ve 365 nm) farklı zaman peryotlarında (15, 30, 45 ve 60 dk.) maruz bırakıldı. Çalışma sonunda larvaların ölüm oranları, komet değeleri ve antioksidan enzim aktiviteleri (SOD, CAT, GST ve GPx) ve MDA seviyeleri araştırıldı. Işınlama süresi arttıkça E. kuehniella larvalarının ölüm oranları artmıştır. Kısa (254 nm) ve uzun (365 nm) dalga boylarını ölüm oranları üzerindeki etkilerini karşılaştırdığımızda 254 nm dalga boyunun 365 nm dalga boyundan daha etkili olduğu belirlenmiştir. Bu durum, ölüm oranı, antioksidan enzim aktivitesi ve MDA seviyesinde de tespit edilmiştir. Komet değerlendirme sonuçlarına göre kuyruk % DNA'sı ve komet kuyruk uzunluğu 254 nm dalga boyutunun tüm uygulama zamanlarında istatistiksel olarak artarken bu değişimler 365 nm UV radyasyonun yalnızca 45. ve 60. dk. larında tespit edilmiştir. Bundan dolayı, kuyruk % DNA'sı ve kuyruk uzunluğu 254 nm dalga boyunda 365 nm dalga boyuna göre daha fazladır. Sonuç olarak, UV radyasyon depo zararlılarının kontrolünde güvenilir bir metod ve çevreye zararlı kimyasal pestisitlere bir alternatif olarak kullanılabilir.Anahtar sözcükler: Ephestia kuehniella, UV radyasyon, ölüm oranı, antioksidan enzimler, komet değerlendirme

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Section: Introductionmentioning

confidence: 99%

UV radiation-induced oxidative stress and DNA damage on Mediterranean flour moth, Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) larvae

Güven

Pandır

Baş

2015

Turkish Journal of Entomology

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“…Boric acid used as an antimicrobial agent induced an increase of chromatid type aberrations at concentrations of 600, 800, 1000 lg/ml in human lymphocytes (Arslan 2004). Additionally, citric acid increased the CAs, SCEs and MN frequency at concentrations of 62.5, 125, 250, 500, 1000 lg/mL in cultured human lymphocytes (Yılmaz et al 2008). Zengin et al (2011) reported that potassium benzoate increased CAs, SCEs and MN frequency at 62.5, 125, 250, 500, 1,000 lg/ml concentrations in cultured human lymphocytes.…”

Section: Discussionmentioning

confidence: 97%

“…Though food preservatives play an important role in the safety of food supply, many studies revealed the potential genotoxic and mutagenic effects of the additives (Hasegawa et al 1984;Schlatter et al 1992;Schiffmann and Schlatter 1992;Yılmaz et al 2008Yılmaz et al , 2009Mamur et al 2010;Zengin et al 2011). Hasegawa et al (1984) reported that SS showed genotoxic effects in Chinese Hamster V79 cells at 200-800 lg/ml concentrations in CAs, SCEs and gene mutation tests.…”

Section: Introductionmentioning

confidence: 99%

Genotoxicity of food preservative sodium sorbate in human lymphocytes in vitro

et al. 2012

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“…Chromosomal aberrations (CAs), sisterchromatid exchanges (SCEs), and micronuclei (MN) tests and comet assay are widely used in cultured human peripheral lymphocytes (Macioszek and Kononowicz 2004;Mamur et al 2010;Rencuzogullari et al 2001;Yuzbasioglu et al 2006;Yılmaz et al 2008b). Comet assay may be used for laboratory investigations as a method to detect the impact of potential germ cell mutagens directly on sperm DNA in vitro.…”

Section: Discussionmentioning

confidence: 99%

DNA damage in human germ cell exposed to the some food additives in vitro

Pandır

2014

Cytotechnology

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The use of food additives has increased enormously in modern food technology but they have adverse effects in human healthy. The aim of this study was to investigate the DNA damage of some food additives such as citric acid (CA), benzoic acid (BA), brilliant blue (BB) and sunset yellow (SY) which were investigated in human male germ cells using comet assay. The sperm cells were incubated with different concentrations of these food additives (50, 100, 200 and 500 μg/mL) for 1 h at 32 °C. The results showed for CA, BA, BB and SY a dose dependent increase in tail DNA%, tail length and tail moment in human sperm when compared to control group. When control values were compared in the studied parameters in the treatment concentrations, SY was found to exhibit the highest level of DNA damage followed by BB > BA > CA. However, none of the food additives affected the tail DNA%, tail length and tail moment at 50 and 100 μg/mL. At 200 μg/mL of SY, the tail DNA% and tail length of sperm were 95.80 ± 0.28 and 42.56 ± 4.66, for BB the values were 95.06 ± 2.30 and 39.56 ± 3.78, whereas for BA the values were 89.05 ± 2.78 and 31.50 ± 0.71, for CA the values were 88.59 ± 6.45 and 13.59 ± 2.74, respectively. However, only the highest concentration of the used food additives significantly affected the studied parameters of sperm DNA. The present results indicate that SY and BB are more harmful than BA and CA to human sperm in vitro.

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Clastogenic effects of food additive citric acid in human peripheral lymphocytes

Cited by 40 publications

References 36 publications

UV radiation-induced oxidative stress and DNA damage on Mediterranean flour moth, Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) larvae

UV radiation-induced oxidative stress and DNA damage on Mediterranean flour moth, Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) larvae

Genotoxicity of food preservative sodium sorbate in human lymphocytes in vitro

DNA damage in human germ cell exposed to the some food additives in vitro

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