Class I integrons containing adhfrItrimethoprim resistance gene cassette in aquaticAcinetobacterspp.

Abstract

The presence of antibiotic resistance gene cassettes in class I integrons was investigated in 24 sulfamethoxazole-resistant and -sensitive Acinetobacter isolates derived from two Danish freshwater trout farms. Integrons were detected in five isolates from one of the fish farms, and their inserts were characterised by DNA sequencing. Each isolate contained a dhfrI gene cassette encoding resistance to trimethoprim and an open reading frame orfC of unknown function identical to the content of an integron previously found in a clinical enterobacterial isolate. Among the five isolates, at least two different strains were differentiated based on phenotypic tests and randomly amplified polymorphic DNA analysis. To our knowledge, this is the first report and characterisation of an integron in environmental bacteria.

Whole‐genome analysis of multi‐drug‐resistant Aeromonas veronii isolated from diseased discus (Symphysodon discus) imported to Korea

“…Thus, they are widely known for their role in acquisition of antibiotic-resistance (Marti, Variatza, & Balcazar, 2014). These two integrons of strain 17ISAe both harboured sul1 gene responsible for resistance to sulfa drugs, similar to previous reports L'Abée-Lund & Sørum, 2001;Petersen et al, 2000;Schmidt, Bruun, Dalsgaard, & Larsen, 2001). of dfrA1-gcuC, a common gene cassette conferring trimethoprim resistance, and sul1 in the integron of strain 17ISAe as described previously (Domingues, da Silva, & Nielsen, 2015;Partridge, Tsafnat, Coiera, & Iredell, 2009).…”

Characterisation of Class 1 Integron among <i>Escherichia coli</i> Isolated from Mansoura University Hospitals in Egypt

“…By DNA amplification of the variable region, 43 isolates were found to carry detectable Class 1-related integrons, [36], which may indicate that the dfrA17-aadA5 array can disseminate by self-transferable plasmids in humans or animals. It was believed that the spread of trimethoprim resistance genes in clinical isolates is mostly associated with insertion of gene cassettes into integrons [37]. In our study, trimethoprim and gentamicin resistances were indicated in different dfrA17-aadA5 carrying E. coli isolates.…”

Antimicrobial Resistance Determinants in Imipenem-nonsusceptible Acinetobacter calcoaceticus-baumannii Complex Isolated in Daejeon, Korea

“…In some instances, carbapenemase genes, such as bla IMP , bla VIM , bla GIM , or bla SIM , are located within class 1 integrons and lead to carbapenem resistance. Multidrug-resistant Acb complex strains have been known to acquire their antimicrobial resistance genes via class 1 integrons that carry single or multiple gene cassettes [9]. …”