Cis-2-decenoic Acid Inhibits S. aureus Growth and Biofilm In Vitro: A Pilot Study

Abstract

Background Cis-2 decenoic acid (C2DA) disperses biofilm in many strains of microorganisms. However, whether C2DA inhibits bacterial growth or has potential to boost the actions of antibiotics is unknown. Questions/purposes We asked whether (1) C2DA inhibited MRSA growth and biofilm, (2) antibiotics increased inhibitory effects, (3) inhibitory concentrations of C2DA were cytotoxic to human cells, and (4) effective concentrations could be delivered from a chitosan sponge drug delivery device. Methods Broth containing seven concentrations of C2DA and six concentrations of either daptomycin, vancomycin, or linezolid was inoculated with a clinical isolate of MRSA and added to a total of 504 coated microtiter plate wells in triplicate (n = 3) for turbidity bacterial growth and crystal violet biofilm mass quantification. We used fibroblast cell viability assays of six C2DA concentrations (n = 4) to evaluate preliminary biocompatibility. We measured the elution of C2DA from a chitosan sponge drug delivery device with two representative loading concentrations (n = 3).

In vitro evaluation of antibacterial activity of phytochemicals and micronutrients against Borrelia burgdorferi and Borrelia garinii

“…It was found to be bacteriostatic at 125–500  μ g ml −1 against methicillin‐resistant Staphylococcus aureus and its biofilm, without significant cytotoxicity on human dermal fibroblasts (Jennings et al . 2012). In separate studies, Davies and Marques observed dispersion of biofilms formed by Pseudomonas aeruginosa PAO1 by using cis‐2‐decenoic acid at a native concentration of 2·5 nmol l −1 .…”

In vitro activity, extraction, separation and structure elucidation of antibiotic produced by Streptomyces anulatus NEAE-94 active against multidrug-resistant Staphylococcus aureus

“…It disperses biofilm in many strains of micro-organisms [39]. C2DA has been reported to inhibit the growth of multidrug-resistant S. aureus and biofilm in vitro [39].…”

“…It disperses biofilm in many strains of micro-organisms [39]. C2DA has been reported to inhibit the growth of multidrug-resistant S. aureus and biofilm in vitro [39]. In 2006, Karanja et al [40] identified non-adecane from Chy 4-10, a novel Streptomyces isolate from Chyulu National Park, by GC-MS. Nonadecane is a component of the bacteriocin produced by a mixed culture of Aeromonas sp.…”

Perspectives on the prevention and treatment of infection for orthopedic tissue engineering applications

“…Cis-2-decenoic acid appears to act as a diffusible signaling molecule that allows biofilm organisms to coordinate gene expression via cell-cell communication. Jennings et al [58] demonstrated in vitro that antibiotics are more effective at preventing biofilm growth when combined with cis-2-decenoic acid. Practically, cis-2-decenoic is challenging to incorporate into a sustained release local delivery vehicle since it is a fatty acid.…”