In vitro activity, extraction, separation and structure elucidation of antibiotic produced by Streptomyces anulatus NEAE-94 active against multidrug-resistant Staphylococcus aureus

El‐Naggar, Noura El‐Ahmady; El-Bindary, A.A.; Abdel-Mogib, Μ.; Nour, Noura Salah

doi:10.1080/13102818.2016.1276412

Cited by 41 publications

(18 citation statements)

References 38 publications

(42 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The obtained peaks were analysed with mass spectral analysis. The spectrum was analysed from the available database in National Institute and Standard Technology (NIST) and the results were interpreted ( El-Naggar et al, 2017 ).…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial and antinematicidal metabolites from Streptomyces cuspidosporus strain SA4 against selected pathogenic bacteria, fungi and nematode

Sholkamy

Muthukrishnan²,

Abdel-Raouf

et al. 2020

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

show abstract

Section: Methodsmentioning

confidence: 99%

Antimicrobial and antinematicidal metabolites from Streptomyces cuspidosporus strain SA4 against selected pathogenic bacteria, fungi and nematode

Sholkamy

Muthukrishnan²,

Abdel-Raouf

et al. 2020

Saudi Journal of Biological Sciences

View full text Add to dashboard Cite

show abstract

“…This compound was potential as a growth inhibitor of C. albicans, Staphylococcus aureus, S. epidermidis, Enterobacter faecalis, and Pseudomonas aeruginosa (Gul and Bakht 2015). Some compounds were identified by GC-MS analysis also presented in the extract of marine Streptomyces ( Sudha and Masilamani 2012;Nandhini et al 2015), extract of Streptomyces anulatus (El-naggar et al 2017), extract of Streptomyces SCA 7 (Saravana et al 2014), and extract of Trichoderma atroviride (Keszler et al 2000). Streptomyces sp.…”

Section: Secondary Metabolite Analysismentioning

confidence: 98%

Growth inhibition of Fusarium solani and F. oxysporum by Streptomyces sasae TG01, and its ability to solubilize insoluble phosphate

et al. 2020

View full text Add to dashboard Cite

Abstract. Sudiana A, Putri A, Napitupulu TP, Purnaningsih I, Idris, Kanti A. 2020. Growth inhibition of Fusarium solani and F. oxysporum by Streptomyces sasae TG01, and its ability to solubilize insoluble phosphate. Biodiversitas 21: 429-435. Actinomycetes have been widely explored for new antibiotic production, but not many studies explore its abilities to inhibit the growth of phytopathogenic fungi and solubilize insoluble phosphate hence stimulate the growth of plants. We isolated Actinomycetes from the soil. Based on morphology, physiology, and 16S rDNA analyses, the isolate is closely related to Streptomyces sasae. The strain was able to inhibit the growth of phytopathogenic fungi Fusarium solani, and Fusarium oxysporum. S. sasae produced secondary metabolites 2-methyl-1,3-dioxolane as the major constituent. The strain assimilated variable carbon sources include L-arabinose, D-fructose, D-glucose, D-mannitol, Lactose, raffinose, L-rhamnose, and sucrose. The strain grew at pH 6.0 to 8.0, and at salinity (1-3%). Their growth was affected by the salinity level. The strain solubilized Ca-P at 1-3% salinity, but their ability to solubilize phosphate was influenced by salinity. The strain was also able to solubilize rock phosphate. Their ability to solubilize less soluble phosphate and inhibit the growth of F. solani and F. oxysporum may imply that this strain is potential for biocontrol agents. The 16S rRNA gene was submitted to DDBJ with the entry number 5df623c1a3c8820021322a36.TG01, and the accession number is LC514451.

show abstract

“…In this study, the fermented cultures were subjected to the extraction process to purify the antibiotic principles. [6]…”

Section: Methodology Fermentation Of Antibiotic Principlesmentioning

confidence: 99%

“…The obtained residues were named [ Table 1], weighed, and checked for antibiotic activity against Staphylococcus aureus (MTCC 1430) by disc diffusion method. [6,7]…”

Section: Extraction Of Antibiotic Residuesmentioning

confidence: 99%

Untitled

Sudhan¹

2019

IJGP

View full text Add to dashboard Cite

Introduction: Three broad-spectrum antibiotic-producing microbes (named IF 32 , IF 52 , and CF 42) were identified and isolated from intestinal region and coelomic fluids of sea cucumber species collected from Kanyakumari district, Tamil Nadu. Biochemical characterization of the isolated microbes and optimization of culture characteristic (for maximum antibiotic productivity) were performed. The present study focuses on extraction and purification of antibiotic principles from the culture medium of the selected isolates. Methodology: The antibiotic fermentation was carried out in the optimized fermentation medium under optimum conditions separately for the isolated species. After separation of the cells by filtration, the fermented culture media were subjected to solvent extraction using 1-butanol, chloroform, ethyl acetate, and hexane. Obtained residues were subjected to antibiotic screening against Staphylococcus aureus (MTCC 1430) by disc plate method. Purification of the selected residues which showed antibiotic activity was done by column chromatography using silica gel as packing material. Collected fractions of the three antibiotic residues were subjected to bioautography to ensure the purification of the antibiotics. Results: The results show that chloroform, 1-butanol, and ethyl acetate are found to be suitable solvents for the extraction of antibiotics from the fermented cultures of IF 32 , IF 52 , and CF 42 , respectively. Bioautography performed using fractions obtained from column chromatographic separation ensured the purification of the antibiotic principles.

show abstract

In vitro activity, extraction, separation and structure elucidation of antibiotic produced by Streptomyces anulatus NEAE-94 active against multidrug-resistant Staphylococcus aureus

Cited by 41 publications

References 38 publications

Antimicrobial and antinematicidal metabolites from Streptomyces cuspidosporus strain SA4 against selected pathogenic bacteria, fungi and nematode

Antimicrobial and antinematicidal metabolites from Streptomyces cuspidosporus strain SA4 against selected pathogenic bacteria, fungi and nematode

Growth inhibition of Fusarium solani and F. oxysporum by Streptomyces sasae TG01, and its ability to solubilize insoluble phosphate

Untitled

Contact Info

Product

Resources

About