Circulating microRNA signatures serve as potential diagnostic biomarkers for <i>Helicobacter pylori</i> infection

Yu, Jie; Xu, Qiaoxia; Zhang, Xiaoyu; Zhu, Miao

doi:10.1002/jcb.27462

Cited by 16 publications

(14 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A plasma sample also offers the opportunity to detect circulating microRNAs (miRNAs) by molecular techniques. For example, the expression of four miR-28-3p, miR-143-3p, miR-151a-3p and miR-148a-3p were shown to be associated with H. pylori infection [ 117 ].…”

Section: Non-invasive Testsmentioning

confidence: 99%

What Is New in Helicobacter pylori Diagnosis. An Overview

Dore

2021

JCM

View full text Add to dashboard Cite

Helicobacter pylori infection remains one of the most prevalent infections worldwide, especially in low-resource countries, and the major risk factor for peptic ulcer and gastric cancer. The “test-and-treat” strategy is recommended by several guidelines and consensus. The choice of testing method is based on patient age, presence of alarm signs and/or symptoms, use of non-steroidal anti-inflammatory drugs, as well as local availability, test reliability, and cost. Culture is the gold standard to detect H. pylori and, possibly, to perform susceptibility testing, however, it requires upper endoscopy and dedicated labs. Recent advances in molecular biology have provided new strategies in detecting infection and antimicrobial resistance without invasive tests. In this review we attempt to offer a comprehensive panorama on the new diagnostic tools and their potential use in clinical settings, in order to accomplish specific recommendations.

show abstract

Section: Non-invasive Testsmentioning

confidence: 99%

What Is New in Helicobacter pylori Diagnosis. An Overview

Dore

2021

JCM

View full text Add to dashboard Cite

show abstract

“…Thus, these four miRNAs combined may constitute a "specific biomarker signature for H. pylori infection". 39…”

Section: H Pylori Infection Was Introduced By Yu Et Al Using High-tmentioning

confidence: 99%

Review: Diagnosis of Helicobacter pylori infection

et al. 2019

View full text Add to dashboard Cite

Endoscopic imaging of the stomach is improving. In addition to narrow band imaging, other methods, for example, blue light imaging and linked color imaging, are now available and can be combined with artificial intelligence systems to obtain information on the gastric mucosa and detect early gastric cancer. Immunohistochemistry is only recommended as an ancillary stain in case of chronic active gastritis without Helicobacter pylori detection by standard staining, and recommendations to exclude false negative H. pylori results have been made. Molecular methods using real‐time PCR, droplet digital PCR, or amplification refractory mutation system PCR have shown a high accuracy, both for detecting H. pylori and for clarithromycin susceptibility testing, and can now be used in clinical practice for targeted therapy. The most reliable non‐invasive test remains the 13C‐urea breath test. Large data sets show that DOB values are higher in women and that the cut‐off for positivity could be decreased to 2.74 DOB. Stool antigen tests using monoclonal antibodies are widely used and may be a good alternative to UBT, particularly in countries with a high prevalence of H. pylori infection. Attempts to improve serology by looking at specific immunodominant antigens to distinguish current and past infection have been made. The interest of Gastropanel® which also tests pepsinogen levels was confirmed.

show abstract

“…Reports showed that the AUC of miR-28-3p was 0.792 (95% confidence interval: 0.689-0.896), and the high expression of miR-28-3p in plasma could be used as a non-invasive and stable biomarker for detecting pulmonary embolism ( 48 ). Yu et al ( 49 ) used qRT-PCR and then analyzed the operating characteristics of the subjects, and found that the expression of miR-28-3p, miR-143-3p, miR-151a-3p and miR-148a-3p were closely related to Helicobacter pylori infection. The above four plasma miRNA groups were expected to be used as non-invasive biomarkers for Helicobacter pylori infection.…”

Section: Discussionmentioning

confidence: 99%

Expression of miR‑28‑3p in patients with Alzheimer's disease before and after treatment and its clinical value

2020

View full text Add to dashboard Cite

Expression of miR-28-3p in patients with Alzheimer's disease (AD) before and after treatment and clinical value of miR-28-3p were determined. There were three groups: 68 AD patients treated with donepezil combined with basic therapy in The People's Hospital of Shouguang collected as an AD group, 70 patients with mild cognitive impairment (MCI) as an MCI group, and 75 healthy people as a normal group. Serum miR-28-3p was detected by qRT-PCR. The Montreal cognitive assessment scale (MoCA), mini mental state examination scale (MMSE), activities of daily living scale (ADL) and homocysteine (Hcy) were adopted to assess patients before and after treatment. miR-28-3p in normal group was significantly lower than that in other two groups, and miR-28-3p in MCI group was significantly lower than that in AD group (P<0.001). miR-28-3p correlated with the course and severity of patients. miR-28-3p in AD group after treatment was significantly lower than that before treatment (P<0.001). ADL and Hcy of AD patients after treatment were significantly lower than before treatment (P<0.05), and MMSE and MoCA after treatment were significantly higher than before treatment (P<0.05). Before and after treatment, miR-28-3p was significantly positively correlated with ADL score and Hcy level, but negatively correlated with MMSE score and MoCA score. Analysis of the working characteristic curve of the patients indicated that miR-28-3p can be used for diagnosis of AD patients. Donepezil therapy may reduce miR-28-3p level to alleviate the symptoms of AD patients, and miR-28-3p level can be used as an early diagnosis and prognosis evaluation of AD patients.

show abstract

Circulating microRNA signatures serve as potential diagnostic biomarkers for Helicobacter pylori infection

Cited by 16 publications

References 36 publications

What Is New in Helicobacter pylori Diagnosis. An Overview

What Is New in Helicobacter pylori Diagnosis. An Overview

Review: Diagnosis of Helicobacter pylori infection

Expression of miR‑28‑3p in patients with Alzheimer's disease before and after treatment and its clinical value

Contact Info

Product

Resources

About