Circulating Genotypes of Dengue-1 Virus in South West India, 2014–2015

Pooja, S; Sabeena, Sasidharanpillai; Revti, Bhaskar; Sanjay, Ramachandran; Anjali, A. K.; Rajendra, K.C.; Aswathyraj, Sushama; Giselle, Dsouza; Maity, Hindol; Arunkumar, Govindakarnavar

doi:10.7883/yoken.jjid.2017.087

Cited by 5 publications

(3 citation statements)

References 16 publications

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“…The data showed the replacement of GIII by GI. This genotype has been reported in other southern states, such as Kerala, Karnataka, and Tamil Nadu [ 18 , 22 ]. All of the five sequences subjected to genotype analysis showed close relatedness to the Kerala (Accession no.…”

Section: Discussionsupporting

confidence: 68%

Association of Dengue Virus Serotypes 1&2 with Severe Dengue Having Deletions in Their 3′Untranslated Regions (3′UTRs)

Maisnam

Billoria²,

Prasad³

et al. 2023

Microorganisms

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Dengue virus infections are recorded as hyper-endemic in many countries, including India. Research pertaining to the reasons for frequent outbreaks and severe dengue is ongoing. Hyderabad city, India, has been recorded as a ‘hotspot’ for dengue virus infections. Dengue virus strains circulating over the past few years in Hyderabad city have been characterized at the molecular level to analyze the serotype/genotypes; 3′UTRs were further amplified and sequenced. The disease severity in patients infected with dengue virus strains with complete and 3′UTR deletion mutants was analyzed. Genotype I of the serotype 1 replaced genotype III, which has been circulating over the past few years in this region. Coincidentally, the number of dengue virus infections significantly increased in this region during the study period. Nucleotide sequence analysis suggested twenty-two and eight nucleotide deletions in the 3′UTR of DENV-1. The eight nucleotide deletions observed in the case of DENV-1 3′UTR were the first reported in this instance. A 50 nucleotide deletion was identified in the case of the serotype DENV-2. Importantly, these deletion mutants were found to cause severe dengue, even though they were found to be replication incompetent. This study emphasized the role of dengue virus 3′UTRs on severe dengue and emerging outbreaks.

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Section: Discussionsupporting

confidence: 68%

Association of Dengue Virus Serotypes 1&2 with Severe Dengue Having Deletions in Their 3′Untranslated Regions (3′UTRs)

Maisnam

Billoria²,

Prasad³

et al. 2023

Microorganisms

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“…A hitherto unpublished sequence from Tamil Nadu (Vellore 2015) aligned as G-I (Asian). (Tripathi et al, 2012;Afreen et al, 2015;Cecilia et al, 2017;Pooja et al, 2017;Yergolkar et al, 2017) Kerala also had genotype I (Asian) circulating (2014,2015).…”

Section: Denv-1 Genotypesmentioning

confidence: 99%

Phylogenetic analysis of partial pre-membrane and envelope sequences of dengue viruses circulating in India

Kasirajan

Mammen

Kannangai

et al. 2019

International Journal of Infectious Diseases

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A B S T R A C TObjective: Phylogenetic characteristics of circulating Indian dengue viruses (DENV) were analysed using partial pre-membrane (PrM) and envelope (E) sequences. An immunodominant region was analysed for mutations, and alignment with common DENV PCR primers and probes was determined. Methods: Published Indian PrM and E DENV sequences were analysed with hitherto unpublished PrM sequences from this study site. Alignments of DENV were checked for mutations in an immunodominant region and against the commonly used PCR primers and probes. Results: All four serotypes of DENV circulate in India. Genotype (G) GIII and GI of DENV-1 co-circulated in the south with significant PrM mutations before and after 2012. DENV-2 American genotype was first reported after which the Cosmopolitan genotype co-circulated with it in the southwest. The Cosmopolitan strain has been the only DENV-2 genotype circulating, although an Asian American genotype was recently reported. Significant mutations were found in the E region of DENV-2 strains. DENV-3 strains were GIII across the country. DENV-4 GI from the south and west has now spread across India. No significant mutations were found for DENV-3 or DENV-4. Indian strains showed mutations in an immunodominant region of the E gene and in the regions targeted by commonly used PCR primers and probes. Conclusions: The genetic variability of Indian DENV with co-circulation of multiple genotypes suggests that genotype surveillance is crucial to determining the composition of dengue vaccines and understanding their contribution to epidemiology, virus fitness and pathogenesis. Some mutations seen in an immunodominant region of the E gene may allow these viruses to evade host immune cells. The mutations in the regions targeted by commonly used primers and probes necessitate higher degeneracy.

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“…Dengue is a ssRNA virus belonging to the Flaviviridae family and is mainly spread by Aedes aegypti and A.albopictus [1][2][3][4]. The dengue virus DNEV genome encodes three structural proteins(C, prM, E)and seven nonstructural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5) [5][6][7].There are four different serotypes of DENV and infection with any of the four serotypes can exhibit extremely severe manifestations [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of the viral structural protein genes of 2018 dengue virus serotype 1 epidemic in Yunnan, Southwest China

Lan

Yun²,

et al. 2020

Preprint

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Background There was a dengue virus serotype 1 (DENV-1) epidemic from October to December in 2018 in Xishuangbanna, Yunnan, southwest China, neighboring Myanmar, Laos and Vietnam. And the molecular characterization, evolution and potential source of DENV from Xishuangbanna were investigate. Methods C (capsid)/prM(premembrane)/E (envelope) genes of DNEV isolated from 87 serum samples obtained from local patients were amplified and sequenced, then evaluated using mutation, phylogenetic and homologous recombination analyses and secondary structure prediction. Results Phylogenetic analysis show that all the DENV strains from Xishuangbanna can grouped in two branch of DENV-1. Out of 52, 50 of isolates were grouped in branch one, and were most similar to Fujian (DQ193572) and Singapore 2016(MF314188) strains. The other two isolates have the closest relationship to Myanmar 2017 (MG679801) . When compared with DENV-1SS (standard strain), we found the amino acid residue of E155 was mutation from T to S,which may related to the virulence of virus and no obvious homologous recombination signals. Secondary structure prediction showed that some changes were found in the helical of MN123849 and MN123854 strains and few changes observed in disordered region. Conclusions This study revealed the molecular characterization of structural genes of xishuangbanna, 2018 epidemic strain, providing a reference for molecular epidemiology, infection, pathogenicity and vaccine development .

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Circulating Genotypes of Dengue-1 Virus in South West India, 2014–2015

Cited by 5 publications

References 16 publications

Association of Dengue Virus Serotypes 1&2 with Severe Dengue Having Deletions in Their 3′Untranslated Regions (3′UTRs)

Association of Dengue Virus Serotypes 1&2 with Severe Dengue Having Deletions in Their 3′Untranslated Regions (3′UTRs)

Phylogenetic analysis of partial pre-membrane and envelope sequences of dengue viruses circulating in India

Molecular characterization of the viral structural protein genes of 2018 dengue virus serotype 1 epidemic in Yunnan, Southwest China

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