Circular RNA circ_0000515 adsorbs miR-542-3p to accelerate bladder cancer progression via up-regulating ILK expression

Abstract

Background: Bladder cancer (BC) is a common cause of cancer-relevant deaths globally. This study is designed to delve into expressions, biological functions and molecular mechanisms of circ_0000515 in BC. Methods: Quantitative real-time polymerase chain reaction was accomplished to examine circ_0000515, miR-542-3p and integrin-linked kinase (ILK) mRNA expressions in BC tissues and cell lines. In RT-4 and RT-112 cells with circ_0000515 depletion and UMUC3 and BIU-87 cells with this circ RNA overexpression, a cell counting kit-8 assay was adopted to monitor the viability. Besides, transwell assay was conducted to detect cell migration and aggressiveness, and luciferase reporter gene assay was applied to probe the interplay among circ_0000515, miR-542-3p and ILK mRNA. Additionally, Besides, the regulatory function of circ_0000515 on miR-542-3p expression was under the assay of quantitative real-time polymerase chain reaction, and western blot was fulfilled to determine the regulative function of circ_0000515/miR-542-3p axis on ILK protein expressions. A xenograft animal was modeled to examine lung metastasis in vivo . Results: Circ_0000515 and ILK expressions were significantly elevated in BC tissues and cell lines, while that of miR-542-3p was dramatically suppressed. Knocking down circ_0000515 could significantly repress the growth, migration and aggressiveness of BC cells while overexpression of circ_0000515 showed opposite effects. Moreover, circ_0000515 knockdown inhibited pulmonary metastasis in vivo . Circ_0000515 was validated to adsorb miR-542-3p, and ILK was testified as the downriver target of miR-542-3p. Circ_0000515 could ascend ILK expression through repressing that of miR-542-3p. Conclusions: Circ_0000515, as a tumor promoter, strengthens the viability, migration and aggressiveness of BC cells via modulating miR-542-3p/ILK axis.

circ_0000515 promotes invasion and migration of breast cancer by attenuating MDM2-mediated FUS ubiquitination and degradation

“…Previous studies demonstrate that circRNAs exhibit pivotal regulatory functions in the development of BC [23][24][25] . In this work, we identi ed 49 DEcircRNAs in BC from GEO databases.…”

“…Interestingly, circ_0000515 was identi ed as a common essential factor with high abundance. Peng et al [23] discovered that circ_0000515 adsorbs miRNA-542-3p to accelerate bladder cancer progression via up-regulating integrin-linked protein kinase expression. However, no investigations have investigated whether circ_0000515 plays a similar role as its parent gene in promoting BC tumorigenic features.…”

LINC00092 Enhances LPP Expression to Repress Thyroid Cancer Development via Sponging miR-542-3p

“…Many studies reveal that miR-542-3p can target different target genes in different cancers such as targeting OTUB1 gene in colorectal cancer [22], ILK gene in bladder cancer [25], and FTSJ2 gene in lung cancer [20]. By TargetScan (https://www.targetscan.org/ vert_71/) prediction, lipoma preferred partner (LPP) was predicted as a target gene of miR-542-3p.…”