Circular DNAs in mitochondria of <i>Vicia faba</i>

Eisner, Galina; Negruk, V. I.; Cherny, Dmitry; Alexandrov, A. A.; Shemyakin, M.F.; Butenko, R. G.

doi:10.1016/0014-5793(84)80875-3

Cited by 3 publications

(1 citation statement)

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“…Electron microscopy and biochemical analysis showed that they were supercoiled [ 13,16], and their replicafive forms have been observed [2,29]. Two plasmids called 1700F and 1420 [ 16] or mt-plasmid 2 and mt-plasmid 3 [28] were found in all lines analysed.…”

Section: Introductionmentioning

confidence: 94%

Sequence and transcription analysis of mitochondrial plasmids isolated from cytoplasmic male-sterile lines of Vicia faba

et al. 1992

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Three mitochondrial plasmids (1704, 1695 and 1478 bp) were isolated from sterile cytoplasms of Vicia faba L. and cloned into a bacterial plasmid vector. Their nucleotide sequence was found to be 99 to 100% homologous to their counterparts isolated from a fertile cytoplasm (J.A. Wahleithner and D.R. Wolstenholme, Curr Genet 12 (1987) 55-76). Several overlapping transcripts were localized in the region which is unique to each of the three plasmids. S1 nuclease mapping indicated for all of them several 3' termini but a unique 5' boundary which was located downstream of the consensus sequence CNTAAGTGANNNNNGAA also found at the transcript 5' boundary of other plant mitochondrial plasmids. Southern blot hybridization with nuclear DNA indicated the presence of nuclear sequences homologous to each plasmid.

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Section: Introductionmentioning

confidence: 94%

Sequence and transcription analysis of mitochondrial plasmids isolated from cytoplasmic male-sterile lines of Vicia faba

et al. 1992

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Diversity of Vicia faba circular mtDNA in whole plants and suspension cultures

Negruk

Eisner²,

Redichkina

et al. 1986

Theoret. Appl. Genetics

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A comparative analysis of the Vicia faba mitochondrial genome in whole plants and in longterm suspension culture has been conducted. Restriction fragment patterns of the mtDNA isolated from these two sources were notably different. Electronmicroscopic analysis also revealed significant differences. Large circular mtDNA patterns shifted from a 37-80 kb subpopulation, which was predominant in whole plants, to 18-34 kb subpopulations although in both classes notable quantities of circular molecules of 80 to 120 kb and more were also found. Both in whole plant and suspension culture cells very large circular DNAs were observed. Some of them had lengths nearly 290 kb and could be considered as evidence of the existence of master chromosomes. The minicircular DNA population was also altered. In the suspension culture we observed a notable increase of percentage of minicircles with sizes near 1 kb. Simultaneously, the percentage of minicircles with sizes near 3.5-10 kb significantly increased in suspension culture cells. In addition, a new peak (10-12 kb) of minicircles appeared. Copy number alterations for some sequences homologous to CCC1A, CCC1B and CCC2 (Negruk et al. 1982, 1985) were shown. Southern hybridization revealed the existence of a family of minicircles having sizes 1.4-2 kb with predominance of CCC1A, CCC1B and CCC2. The copy numbers of CCC1B and some minor minicircles was changed in the suspension culture when compared with the whole plants.

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