Sequence and transcription analysis of mitochondrial plasmids isolated from cytoplasmic male-sterile lines of Vicia faba

Flamand, Marie; Goblet, Jean Pierre; Duc, Gérard; Briquet, Michel; Boutry, Marc

doi:10.1007/bf00040524

Cited by 25 publications

(3 citation statements)

References 28 publications

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“…The existence of CRISPR repeats in mitochondria was suggested in one of the earliest publications on the subject (the same article described CRISPR in cyanobacteria for the first time) [ 19 ]. The authors used a set of previously published data on the sequencing of mitochondrial plasmids from Vicia faba L. beans [ 20 ], and their conclusions were further cited by Mojica et al [ 21 ], but these observations were not confirmed in later studies [ 8 ].…”

Section: A History Of the Discovery Of The Main Components Of The Cri...mentioning

confidence: 99%

CRISPR–Cas9: A History of Its Discovery and Ethical Considerations of Its Use in Genome Editing

Gostimskaya

2022

Biochemistry Moscow

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The development of a method for genome editing based on CRISPR–Cas9 technology was awarded The Nobel Prize in Chemistry in 2020, less than a decade after the discovery of all principal molecular components of the system. For the first time in history a Nobel prize was awarded to two women, Emmanuelle Charpentier and Jennifer Doudna, who made key discoveries in the field of DNA manipulation with the CRISPR–Cas9 system, so-called “genetic scissors”. It is difficult to overestimate the importance of the technique as it enables one not only to manipulate genomes of model organisms in scientific experiments, and modify characteristics of important crops and animals, but also has the potential of introducing revolutionary changes in medicine, especially in treatment of genetic diseases. The original biological function of CRISPR–Cas9 system is the protection of prokaryotes from mobile genetic elements, in particular viruses. Currently, CRISPR–Cas9 and related technologies have been successfully used to cure life-threatening diseases, make coronavirus detection tests, and even to modify human embryo cells with the consequent birth of babies carrying the introduced modifications. This intervention with human germplasm cells resulted in wide disapproval in the scientific community due to ethical concerns, and calls for a moratorium on inheritable genomic manipulations. This review focuses on the history of the discovery of the CRISPR–Cas9 system with some aspects of its current applications, including ethical concerns about its use in humans.

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Section: A History Of the Discovery Of The Main Components Of The Cri...mentioning

confidence: 99%

CRISPR–Cas9: A History of Its Discovery and Ethical Considerations of Its Use in Genome Editing

Gostimskaya

2022

Biochemistry Moscow

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“…Similarly, mitogenomes of Trifolium L. are relatively compact (294.9–348.7 kb) (Choi, Ruhlman, & Jansen, 2020). In addition to the mitogenome, small mt plasmids were discovered in V. faba (Flamand et al., 1992; Flamand et al., 1993; Wahleithner & Wolstenholme, 1987).…”

Section: Introductionmentioning

confidence: 99%

Born in the mitochondrion and raised in the nucleus: evolution of a novel tandem repeat family in Medicago polymorpha (Fabaceae)

et al. 2022

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Plant nuclear genomes harbor sequence elements derived from the organelles (mitochondrion and plastid) through intracellular gene transfer (IGT). Nuclear genomes also show a dramatic range of repeat content, suggesting that any sequence can be readily amplified. These two aspects of plant nuclear genomes are well recognized but have rarely been linked. Through investigation of 31 Medicago taxa we detected exceptionally high post-IGT amplification of mitochondrial (mt) DNA sequences containing rps10 in the nuclear genome of Medicago polymorpha and closely related species. The amplified sequences were characterized as tandem arrays of five distinct repeat motifs (2157, 1064, 987, 971, and 587 bp) that have diverged from the mt genome (mitogenome) in the M. polymorpha nuclear genome. The mt rps10-like arrays were identified in seven loci (six intergenic and one telomeric) of the nuclear chromosome assemblies and were the most abundant tandem repeat family, representing 1.6-3.0% of total genomic DNA, a value approximately three-fold greater than the entire mitogenome in M. polymorpha. Compared to a typical mt gene, the mt rps10-like sequence coverage level was 691.5-7198-fold higher in M. polymorpha and closely related species. In addition to the post-IGT amplification, our analysis identified the canonical telomeric repeat and the species-specific satellite arrays that are likely attributable to an ancestral chromosomal fusion in M. polymorpha. A possible relationship between chromosomal instability and the mt rps10-like tandem repeat family in the M. polymorpha clade is discussed.

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“…In broad bean (rViciafaba L.), three supercoiled plasmids, mtpl (1704 bp), mtp2 (1695 bp) and mtp3 (1478 bp), have been described in fertile (5,6) as well as male sterile (7) lines. They all are transcribed (7) and replicative forms have been observed by electron microscopy (8). Plasmids mtpl and mtp2 share more than 80% homology along two-thirds of their sequences but the region transcribed is the non-homologous portion.…”

Section: Introductionmentioning

confidence: 99%

Variant mitochondrial plasmids of broad bean arose by recombination and are controlled by the nuclear genome

Flamand

Duc²,

Goblet³

et al. 1993

Nucl Acids Res

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Various cytoplasms of broad bean contain three mitochondrial plasmids (mtp1, 2 and 3), previously described. In cytoplasm 350 we have observed several additional mitochondrial plasmids, varying in number and in identity according to the nuclear background. Replacement of the nucleus by backcrossing led to the appearance or disappearance of additional plasmids, indicating that the nuclear genome controls either the creation or the copy level of mitochondrial plasmids. Analysis of eight variant additional plasmids (mtp4-11) suggests that they all result from a double recombination event between mtp1 and mtp2. In all cases, one recombination point was located within a 276-bp sequence, identical in both plasmids. For 7 plasmids, the region in which the second recombination event occurred could be narrowed down to a short stretch containing imperfect tandem repeats of a 31-bp motif. The largest sequence shared by the recombination regions was hexanucleotide GCGACG.

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Sequence and transcription analysis of mitochondrial plasmids isolated from cytoplasmic male-sterile lines of Vicia faba

Cited by 25 publications

References 28 publications

CRISPR–Cas9: A History of Its Discovery and Ethical Considerations of Its Use in Genome Editing

CRISPR–Cas9: A History of Its Discovery and Ethical Considerations of Its Use in Genome Editing

Born in the mitochondrion and raised in the nucleus: evolution of a novel tandem repeat family in Medicago polymorpha (Fabaceae)

Variant mitochondrial plasmids of broad bean arose by recombination and are controlled by the nuclear genome

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