Molecular hybridization analysis of cloned Vicia faba minicircular DNA sequences

Negruk, V. I.; Гончарова, Н. П.; Gelnin, L. G.; Mardamshin, A. G.

doi:10.1007/bf00332944

Cited by 10 publications

(2 citation statements)

References 21 publications

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“…MtDNA was cut by restriction endonucleases in the presence of RNase, electrophoresed in an agarose gel, and transferred by capillarity to a Hybond-N membrane (Amersham). Prehybridization was carried out for 5 h at 42°C in 50% formamide, 5 xSSC, 5 xDenhardt's, 1% SDS, 250 itg/ml salmon sperm DNA.…”

Section: Southern Blot Hybridizationmentioning

confidence: 99%

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Variant mitochondrial plasmids of broad bean arose by recombination and are controlled by the nuclear genome

Flamand

Duc²,

Goblet³

et al. 1993

Nucl Acids Res

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Various cytoplasms of broad bean contain three mitochondrial plasmids (mtp1, 2 and 3), previously described. In cytoplasm 350 we have observed several additional mitochondrial plasmids, varying in number and in identity according to the nuclear background. Replacement of the nucleus by backcrossing led to the appearance or disappearance of additional plasmids, indicating that the nuclear genome controls either the creation or the copy level of mitochondrial plasmids. Analysis of eight variant additional plasmids (mtp4-11) suggests that they all result from a double recombination event between mtp1 and mtp2. In all cases, one recombination point was located within a 276-bp sequence, identical in both plasmids. For 7 plasmids, the region in which the second recombination event occurred could be narrowed down to a short stretch containing imperfect tandem repeats of a 31-bp motif. The largest sequence shared by the recombination regions was hexanucleotide GCGACG.

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Section: Southern Blot Hybridizationmentioning

confidence: 99%

“…Membranes were washed three times for 5 min in 2 x SSC, 0.1 % SDS at room temperature, for 15 min in 2 x SSC, 0.1% SDS at 500C, and for 15 mi in 0. 5 DNA sequencing DNA was sequenced from single-stranded DNA by the dideoxy method (12). Primers were 14 to 16-mer oligonucleotides scattered through the mt plasmids.…”

Section: Southern Blot Hybridizationmentioning

confidence: 99%

Variant mitochondrial plasmids of broad bean arose by recombination and are controlled by the nuclear genome

Flamand

Duc²,

Goblet³

et al. 1993

Nucl Acids Res

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The Mitochondrial Genome of Plants

Lonsdale

Grienenberger

1992

Plant Gene Research

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Characterization of Vicia faba mitochondrial genomes with normal coxII and coxII-orf 192 chimeric genes

Negruk

Zeinalov

Gladky

et al. 1991

Theoret. Appl. Genetics

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Southern hybridization analysis of mitochondrial genomes from different lines and cultivars of Vicia faba, with respect to variability of the coxII gene sequence, revealed two predominant types of mitochondrial genomes. The type I mitochondrial genome contained the coxII gene sequence in a 6.5-kb BamHI fragment. Type II had two copies of the coxII sequence: the first in a 6.5-kb and the second in a 2.6-kb BamHI fragment. The second copy was represented by a coxII-orf192 chimeric gene. We found several pure lines with type I and type II mitochondrial genomes. Each type of genome was stably inherited. No chimeric gene was found in mitochondria of the male-sterile line cms447. Nucleotide sequences of Vicia faba mitochondrial DNA (mtDNA) containing normal and chimeric coxII genes are presented. The sequence of the normal coxII gene was compared to the coxII gene from mitochondria of Pisum sativum. The similarity of nucleotide sequences and of predicted amino acid sequences between these two genes was more than 98%. A very high similarity between transcription initiation and termination signals was also observed. The sequence of the chimeric gene was characterized at the 5' end by the almost complete sequence of the normal coxII gene, up to the fifth nucleotide before the termination codon. The 3' end of the chimeric gene was represented by the 3' part of an orf previously called orf128+. The full size of this orf was 576 nucleotides, and the full size of the predicted polypeptide was 192 amino acid residues. Therefore, this orf can be finally called orf192. Northern hybridization analysis showed that orf192 was actively transcribed into a 1.4-kb transcript. The chimeric gene was also transcribed into a minor transcript of about 3 kb. Comparative analysis of the normal coxII gene and orf192 supported the suggestion that the chimeric gene resulted from nonhomologous recombination.

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Molecular hybridization analysis of cloned Vicia faba minicircular DNA sequences

Cited by 10 publications

References 21 publications

Variant mitochondrial plasmids of broad bean arose by recombination and are controlled by the nuclear genome

Variant mitochondrial plasmids of broad bean arose by recombination and are controlled by the nuclear genome

The Mitochondrial Genome of Plants

Characterization of Vicia faba mitochondrial genomes with normal coxII and coxII-orf 192 chimeric genes

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