CircRNA NALCN acts as an miR-493-3p sponge to regulate PTEN expression and inhibit glioma progression

Liu, Yi; Chen, Simin; Peng, Gang; Liao, Yixiang; Fan, Xue‐Gong; Zhang, Zuping; Shen, Chenfu

doi:10.1186/s12935-021-02001-y

Cited by 14 publications

(7 citation statements)

References 36 publications

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“…Although isoproterenol has been reported to have an inhibitory effect on gastric cancer cells, no increase in level of miR-493-3p was discovered as a result of isoproterenol treatment. In cancer patients, the reduced expression of miR-493-3p is linked to bigger tumors, worse division, and shorter survival periods [ 21 – 23 ]. These studies suggested that miR-493-3p might serve as a candidate tumor suppressor in gastric cancer.…”

Section: Discussionmentioning

confidence: 99%

Propofol-Induced miR-493-3p Inhibits Growth and Invasion of Gastric Cancer through Suppression of DKK1-Mediated Wnt/β-Catenin Signaling Activation

et al. 2023

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Purpose. Gastric cancer (GC) is one of the most common malignant tumors and also one of the most deadly tumors. In recent years, studies have shown that propofol can inhibit the proliferation and metastasis of many tumor cells. In the present study, we aimed to investigate the underlying mechanism of propofol inhibition of the growth and invasion of GC cells. Methods. Human gastric cancer cell line SGC-7901 and human normal gastric epithelial cell GES-1 were cultured in high-glucose Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum at 37°C with 5% CO2. Propofol of different concentrations (0, 2, 5, and 10 μg/mL) was used to treat SGC-7901, and miR-493-3p inhibitor was transfected into SGC-7901. The cell proliferation of SGC-7901 was analyzed by MTT as well as colony formation assay. The qRT-PCR was used to assess the expression of mRNA for key genes. We examined the protein expression of DKK1 and relative markers with western blot. Putative binding places of miR-493-3p on the 3 ′ -untranslated area of DKK1 were predicted using bioinformatics and dual-luciferase method. Results. Propofol prohibited phenotypic features of GC, according to our findings. Furthermore, research into the underlying mechanisms of propofol’s suppressive effects in GC cell proved that propofol therapy improved the degrees of expression of the potential tumor suppressor miR-493-3p. The inhibiting properties of propofol on GC cell development, migration, and invasion were abolished when propofol-induced miR493-3p was silenced with anti-miR-493-3p. We also found that this drug reversed epithelial-mesenchymal transformation in SGC-7901 cells via inducing miR-493-3p. Propofol-induced miR-493-3p decreases GC cell development via targeting DKK1 and hence inhibits Wnt/β-catenin signaling, according to these findings. Conclusion. Propofol-induced miR-493-3p decreased GC cell development via targeting DKK1 and hence inhibited Wnt/β-catenin signaling, according to these findings.

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Section: Discussionmentioning

confidence: 99%

Propofol-Induced miR-493-3p Inhibits Growth and Invasion of Gastric Cancer through Suppression of DKK1-Mediated Wnt/β-Catenin Signaling Activation

et al. 2023

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“…The existing researches of miR-493-3p are mainly focused on tumor/cancer [20][21][22] , renal brosis/injury [23][24] , cerebral ischemia and reperfusion injury [25] . Besides, miR-493-3p was reported to affect lipolysis in adipocytes of sheep.…”

Section: Discussionmentioning

confidence: 99%

MiR-493-3p inhibits proliferation and promotes differentiation of porcine muscle satellite cells through MKK7/JNK axis

Wu,

Wang,

Sun

et al. 2023

Preprint

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Background Skeletal muscle is one of the primary productions in pig industry, and the myogenesis represents one of the main economic traits of pig. MicroRNA (miRNA) plays critical roles in myogenesis of porcine, however, there are a large number of miRNAs that have not been studied in regulating myogenesis. Here, the effects of miR-493-3p on porcine myogenesis was examined. Results The miR-493-3p is strongly enriched in skeletal muscle, especially in the slow-twitch muscle fibers soleus. We further demonstrated that miR-493-3p significantly inhibits the proliferation of porcine primary muscle satellite cells (MSCs). In contrast, miR-493-3p significantly promotes the differentiation of porcine primary MSCs. Mechanistically, bioinformatic analysis and dual luciferase assay results show that the MAP kinase kinase 7 (MKK7) is a direct target of miR-493-3p. Moreover, miR-493-3p inhibits MKK7 expression, leading to the decreased phosphorylated level of JNK (p-JNK), thus regulate myogenesis. Conclusions Overall, these findings suggest that miR-493-3p inhibits proliferation and promotes differentiation of porcine MSCs via inhibiting MKK7/JNK axis. These finding helps to understand the development network of skeletal muscle regulated by miRNA, and provides a theoretical basis for improving pork production.

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“…The identification of sensitive biomarkers is very important for the improvements of clinical outcome of GC patients ( 18 ). In recent years, growing studies highlighted the potential of circRNAs (CircRNA NALCN, Circ_0001588, and CircFAM73A) used as novel biomarkers for tumor patients, including GC ( 19 – 21 ). Here, the authors identified a novel GC-related circRNA, circ-0002570 whose back-splicing junctions was demonstrated by Sanger sequencing.…”

Section: Discussionmentioning

confidence: 99%

Circular RNA Circ-0002570 Accelerates Cancer Progression by Regulating VCAN via MiR-587 in Gastric Cancer

et al. 2021

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BackgroundCircular RNAs (circRNAs) are closely associated with the occurrences and progress of gastric cancer (GC). We aimed to delve into the function and pathological mechanism of Circular RNA-0002570 (circ-0002570) in GC progression.MethodsCircRNAs differentially expressed in GC were screened using bioinformatics technology. The expression of circ-0002570 was detected in GC specimens and cells via qRT-PCR, and the prognostic values of circ-0002570 were determined. The functional roles of circ-0002570 on proliferation, migration, and invasion in GC cells were explored in vitro and in vivo. Interaction of circ-0002570, miR-587, and VCAN was confirmed by dual-luciferase reporter assays, Western blotting, and rescue experiments.ResultsCirc-0002570 expression was distinctly increased in GC tissues compared to adjacent normal specimens, and GC patients with higher circ-0002570 expressions displayed a short survival. Functionally, knockdown of circ-0002570 resulted in the inhibition of cell proliferation, migration, and invasion, and suppressed tumor growth in vivo. Mechanistically, miR-587 was sponged by circ-0002570. VCAN expression in NSCLC was directly inhibited by miR-587. Overexpression of circ-0002570 prevented VCAN from miR-587-mediated degradation and thus facilitated GC progression.ConclusionThe circ-0002570-miR-587-VCAN regulatory pathway promoted the progression of GC. Our findings provided potential new targets for the diagnosis and therapy of GC.

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CircRNA NALCN acts as an miR-493-3p sponge to regulate PTEN expression and inhibit glioma progression

Cited by 14 publications

References 36 publications

Propofol-Induced miR-493-3p Inhibits Growth and Invasion of Gastric Cancer through Suppression of DKK1-Mediated Wnt/β-Catenin Signaling Activation

Propofol-Induced miR-493-3p Inhibits Growth and Invasion of Gastric Cancer through Suppression of DKK1-Mediated Wnt/β-Catenin Signaling Activation

MiR-493-3p inhibits proliferation and promotes differentiation of porcine muscle satellite cells through MKK7/JNK axis

Circular RNA Circ-0002570 Accelerates Cancer Progression by Regulating VCAN via MiR-587 in Gastric Cancer

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