Circular RNA Circ-0002570 Accelerates Cancer Progression by Regulating VCAN via MiR-587 in Gastric Cancer

Yang, Lei; Zhou, Yongning; Zeng, Miaomiao; Zhou, Nan; Wang, Binsheng; Li, Bo; Zhu, Xiaozhong; Guan, Qun; Chai, Chen

doi:10.3389/fonc.2021.733745

Cited by 10 publications

(11 citation statements)

References 39 publications

(34 reference statements)

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“…Of course, different circRNAs have also been shown to have different effects. Some circRNAs mediate the progression of GC as tumor‐promoting factors, such as circ‐0002570 21 and circC6orf132, 22 and some mediate GC development as tumor‐inhibiting factors, such as circ‐TNPO3 23 and circGSK3B. 24 In this, the role of highly expressed circ_0006089 in GC progression was verified.…”

Section: Discussionmentioning

confidence: 81%

circ_0006089 promotes gastric cancer growth, metastasis, glycolysis, and angiogenesis by regulating miR‐361‐3p/TGFB1

et al. 2022

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Circular RNA (circRNA) participates in a variety of pathophysiological processes, including the development of gastric cancer (GC). However, the role of circ_0006089 in GC progression and its underlying molecular mechanism need to be further revealed.Quantitative real-time PCR was utilized for detecting circ_0006089, microRNA (miR)-361-3p and transforming growth factor-β1 (TGFB1) expression. The interaction between miR-361-3p and circ_0006089 or TGFB1 was confirmed using a dual-luciferase reporter assay and an RNA immunoprecipitation (RIP) assay. Cell proliferation, metastasis, apoptosis, and angiogenesis were determined using colony formation assay, EdU assay, transwell assay, flow cytometry, and tube formation assay. Cell glycolysis was evaluated by detecting glucose consumption, lactate production, and ATP levels. In addition, western blot (WB) analysis was used to measure protein expression.Xenograft tumor models were used to assess the effect of circ_0006089 knockdown on GC tumorigenesis. circ_0006089 had been found to be upregulated in GC tissues and cells, and it could act as an miR-361-3p sponge. circ_0006089 knockdown suppressed GC proliferation, metastasis, glycolysis, angiogenesis, and increased apoptosis, while this effect could be revoked by miR-361-3p inhibitor. TGFB1 was targeted by miR-361-3p, and its overexpression reversed the effects of miR-361-3p on GC cell function. Also, circ_0006089 promoted TGFB1 expression via sponging miR-361-3p. Animal experiments showed that silenced circ_0006089 inhibited GC tumorigenesis through the miR-361-3p/TGFB1 pathway. Our results revealed that the circ_0006089/miR-361-3p/TGFB1 axis contributed to GC progression, confirming that circ_0006089 might be a potential therapeutic target for GC.

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Section: Discussionmentioning

confidence: 81%

circ_0006089 promotes gastric cancer growth, metastasis, glycolysis, and angiogenesis by regulating miR‐361‐3p/TGFB1

et al. 2022

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“…Te luciferase activity assay was implemented on the grounds of the foregoing methods [22]. Cloning of mutant (WT) or wild-type (MUT) sequences of circSMARCA5 and PTEN 3′ UTR was into pmirGLO vector (Promega, USA).…”

Section: Te Luciferase Activity Assaymentioning

confidence: 99%

[Retracted] Circular RNA SMARCA5 Modulates Epithelial‐Mesenchymal Transformation, Proliferation, and Metastasis of Nasopharyngeal Carcinoma Cells via microRNA‐582‐3p/Phosphatase and Tensin Homolog Axis

Wang

Ren

2023

Evidence-Based Complementary and Alternative Medicine

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The action mechanism in which circular RNA (circ) SMARCA5 targeted nasopharyngeal carcinoma (NPC) cell proliferation, migration, invasion, and apoptosis via microRNA (miR)-582-3p/phosphatase and tensin homolog (PTEN) axis was explored. The examination was performed via reverse transcription-quantitative polymerase chain reaction (RT-qPCR), discovering that circSMARCA5 was elevated while miR-582-3p was silenced in NPC tissues and cells. E-cadherin and N-cadherin were detected. The results illustrated transfection with si-circSMARCA5 or miR-582-3p-mimic was available to repress cancer cell advancement, and E-cadherin was augmented. Transfection with pcDNA 3.1-circSMARCA5 or miR-582-3p-inhibitor was available to accelerate cancer cell advancement, and N-cadherin was augmented. MiR-582-3p-inhibitor blocked the suppression of si-circSMARCA5 on NPC. The si-PTEN blocked the malignant behavior of pcDNA 3.1-circSMARCA5 against NPC. The binding sites between circSMARCA5 and miR-582-3p and between miR-582-3p and PTEN were verified. Linear analysis results illuminated the expression pattern of circSMARCA5 was opposite to miR-582-3p, while the expression pattern of circSMARCA5 was positively associated with PTEN. In brief, the results of the research clarified circSMARCA5 modulated NPC cells’ vital movement via the miR-582-3p/PTEN molecular axis.

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“… 21 Circ_0002570 knockdown could suppress cell proliferation and metastasis in vitro, and restrain tumor growth in vivo. 22 Circ‐PTPDC1 was overexpressed in GC, and deficiency of circ‐PTPDC1 inhibited GC progression. 23 In our study, hsa_circ_0043691 abundance was higher in GC tissue samples and cells, which was similar to previous research.…”

Section: Discussionmentioning

confidence: 98%

“…For example, overexpression of circHECTD1 advanced GC cell malignant phenotype by energizing glutaminolysis metabolites 21 . Circ_0002570 knockdown could suppress cell proliferation and metastasis in vitro, and restrain tumor growth in vivo 22 . Circ‐PTPDC1 was overexpressed in GC, and deficiency of circ‐PTPDC1 inhibited GC progression 23 .…”

Section: Discussionmentioning

confidence: 99%

Knockdown of hsa_circ_0043691 restrains the progression of gastric cancer by decoying miR‐1294 to target pre‐leukemia transcription factor 3

Gong

Jiang

2022

Clinical Laboratory Analysis

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As the most frequent malignant tumor, gastric cancer (GC) is a major cause of tumor-related death. 1,2 Because of the unconspicuous early symptoms, many GC patients were diagnosed at advanced stages. 3 Although GC can be treated by surgical resection in the clinic, its invasive metastasis greatly increased the difficulty of operation. 4 Thus, it is imperative to investigate the underlying molecular mechanism of GC genesis and progression and develop a new therapeutic regimen.Circular RNA (circRNAs) are characterized by the closed-loop structure and the absence of 5′heads or 3′polyadenylated tail, 5 which made them more stable and resistant to the degradation of exonuclease. 6 Due to the superior characteristics of circRNA, it was found to exert vital effects in the research of new tumor biomarkers. 7 Currently, multiple circRNAs have been found to play an important role in regulating the progress of cell biology in various human diseases. 8,9 Consequently, circRNAs may be promising biomarkers for the management of diverse human diseases.

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Circular RNA Circ-0002570 Accelerates Cancer Progression by Regulating VCAN via MiR-587 in Gastric Cancer

Cited by 10 publications

References 39 publications

circ_0006089 promotes gastric cancer growth, metastasis, glycolysis, and angiogenesis by regulating miR‐361‐3p/TGFB1

circ_0006089 promotes gastric cancer growth, metastasis, glycolysis, and angiogenesis by regulating miR‐361‐3p/TGFB1

[Retracted] Circular RNA SMARCA5 Modulates Epithelial‐Mesenchymal Transformation, Proliferation, and Metastasis of Nasopharyngeal Carcinoma Cells via microRNA‐582‐3p/Phosphatase and Tensin Homolog Axis

Knockdown of hsa_circ_0043691 restrains the progression of gastric cancer by decoying miR‐1294 to target pre‐leukemia transcription factor 3

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