Circadian Regulation and Clock-Controlled Mechanisms of Glycerophospholipid Metabolism from Neuronal Cells and Tissues to Fibroblasts

Guido, Mario E.; Monjes, Natalia M.; Wagner, Paula M.; Salvador, Gabriela Alejandra

doi:10.1007/s12035-021-02595-4

Cited by 21 publications

(22 citation statements)

References 233 publications

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“…Changes in the concentration of glycerophospholipids indicate transformations in cell membrane composition and permeability. The synthesis and degradation of glycerophospholipids are the most highly regulated metabolisms across the 24 h cycle in terms of total lipid content and enzyme expression and activity in individual cells (Guido et al, 2022). It has been confirmed that the functions of glycerophospholipid metabolism in the nucleus is modulated in response to diverse external cues that impact nuclear functions (DNA methylation, histone modifications, chromatin structure, etc.)…”

Section: Discussionmentioning

confidence: 91%

Integrated Proteomics and Metabolomics Analysis in Pregnant Rat Hippocampus After Circadian Rhythm Inversion

Lin

Sun²,

Dai³

et al. 2022

Front. Physiol.

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To investigate the changes in proteins, metabolites, and related mechanisms in the hypothalamus of pregnant rats after circadian rhythm inversion during the whole pregnancy cycle. A total of 12 Wistar female rats aged 7 weeks were randomly divided into control (six rats) and experimental (six rats) groups at the beginning of pregnancy. The control group followed a 12-h light and dark cycle (6 a.m. to 6 p.m. light, 6 p.m. to 6 a.m. dark the next day), and the experimental group followed a completely inverted circadian rhythm (6 p.m. to 6 a.m. light the next day, 6 a.m. to 6 p.m. dark). Postpartum data were collected until 7–24 h after delivery, and hypothalamus samples were collected in two groups for quantitative proteomic and metabolism analyses. The differential proteins and metabolites of the two groups were screened by univariate combined with multivariate statistical analyses, and the differential proteins and metabolites enriched pathways were annotated with relevant databases to analyze the potential mechanisms after circadian rhythm inversion. A comparison of postpartum data showed that circadian rhythm inversion can affect the number of offspring and the average weight of offspring in pregnant rats. Compared with the control group, the expression of 20 proteins and 37 metabolites was significantly changed in the experimental group. The integrated analysis between proteins and metabolites found that RGD1562758 and lysophosphatidylcholine acyltransferase 1 (LPCAT1) proteins were closely associated with carbon metabolism (choline, NAD+, L-glutamine, theobromine, D-fructose, and pyruvate) and glycerophospholipid metabolism (choline, NAD+, L-glutamine, phosphatidylcholine, theobromine, D-fructose, pyruvate, and arachidonate). Moreover, the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the differential metabolites enriched in adenosine triphosphate (ATP)–binding cassette (ABC) transporters. Our study suggested that circadian rhythm inversion in pregnant rats may affect the numbers, the average weight of offspring, and the expressions of proteins and metabolism in the hypothalamus, which may provide a comprehensive overview of the molecular profile of circadian rhythm inversion in pregnant groups.

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Section: Discussionmentioning

confidence: 91%

Integrated Proteomics and Metabolomics Analysis in Pregnant Rat Hippocampus After Circadian Rhythm Inversion

Lin

Sun²,

Dai³

et al. 2022

Front. Physiol.

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“…The synthesis and degradation of glycerophospholipids (GPLs) exhibit an oscillation in metabolisms with a 24 h periodicity 78 . Moreover in quiescent cells, the Per1 gene is tightly involved in GPL synthesis regulation 79 . While in proliferating cells, targeting time‐dependent high‐level redox and low‐level GPL state revealed a potential efficient therapeutic pathway in chemotherapy 80 .…”

Section: Internal Characteristics Of the Glioma Circadian Clockmentioning

confidence: 99%

“…81 Under circadian disruption, more macrophages were recruited in the TME, genes involved in lipogenesis, and glucose uptake was upregulated. 79,81…”

Section: Metabolismmentioning

confidence: 99%

“… 78 Moreover in quiescent cells, the Per1 gene is tightly involved in GPL synthesis regulation. 79 While in proliferating cells, targeting time‐dependent high‐level redox and low‐level GPL state revealed a potential efficient therapeutic pathway in chemotherapy. 80 Constant light, which leads to circadian disruption, promotes anabolic metabolism.…”

Section: Internal Characteristics Of the Glioma Circadian Clockmentioning

confidence: 99%

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Insights about circadian clock in glioma: From molecular pathways to therapeutic drugs

Wang

Chen

2022

CNS Neurosci Ther

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Glioma is characterized as the most aggressive brain tumor that occurred in the central nervous system. The circadian rhythm is an essential cyclic change system generated by the endogenous circadian clock. Current studies found that the circadian clock affects glioma pathophysiology. It is still controversial whether the circadian rhythm disruption is a cause or an effect of tumorigenesis. This review discussed the association between cell cycle and circadian clock and provided a prominent molecular theoretical basis for tumor therapy. We illustrated the external factors affecting the circadian clock including thermodynamics, hypoxia, post‐translation, and microRNA, while the internal characteristics concerning the circadian clock in glioma involve stemness, metabolism, radiotherapy sensitivity, and chemotherapy sensitivity. We also summarized the molecular pathways and the therapeutic drugs involved in the glioma circadian rhythm. There are still many questions in this field waiting for further investigation. The results of glioma chronotherapy in sensitizing radiation therapy and chemotherapy have shown great therapeutic potential in improving clinical outcomes. These findings will help us further understand the characteristics of glioma pathophysiology.

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“…The circadian clock is an evolutionarily conserved 24 h oscillator rhythm driven by endogenous clock genes to anticipate and adapt to the external environment in eukaryotic life, from cyanobacteria to mammals [ 20 , 21 , 22 , 23 ]. The biochemical, physiological, and behavioral functions of organisms are controlled by a group of autoregulatory transcription–translation genes [ 24 , 25 , 26 ]. In mammals, the circadian pacemaker is composed of interlocked feedback loops with the core transcription factors CLOCK and BMAL1 , which drive the transcription of the Period ( PER1 , PER2 ) and Cryptochrome ( CRY1 , CRY2 ) genes [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Generation of Endogenous Promoter-Driven Luciferase Reporter System Using CRISPR/Cas9 for Investigating Transcriptional Regulation of the Core Clock Gene BMAL1

Sun

et al. 2022

Biomedicines

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Humans and other organisms are continuously exposed to thousands of chemicals through the atmosphere, drinking water, food, or direct contact. A large proportion of such chemicals are present in very low concentrations and may have synergistic effects, even at their no-observed-adverse-effect level (NOAEL). Complex mixtures of contaminants are very difficult to assess by traditional toxicological methods. There is increasing attention on how different pollutants induce adverse physiological functions in the human body through effects on the circadian rhythm. However, it is very difficult to screen for compounds with circadian-rhythm-disrupting effects from a large number of chemicals or their complex mixtures. We established a stable firefly luciferase reporter gene knock-in U2-OS cell line by CRISPR/Cas9 to screen circadian-rhythm-disrupting pollutants. The luciferase gene was inserted downstream of the core clock gene BMAL1 and controlled by an endogenous promoter. Compared to detection systems using exogenous promoters, these cells enable the detection of compounds that interfere with the circadian rhythm system mediated by BMAL1 gene expression. The U2-OS knock-in cells showed BMAL1 and luciferase activity had parallel changes when treated with BMAL1 inhibitor and activator. Furthermore, the luciferase reporter gene has high sensitivity and is faster and more cost-effective than classic toxicology methods. The knock-in cell line can be used for high-throughput and efficient screening of circadian-rhythm-disrupting chemicals such as drugs and pollutants.

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Circadian Regulation and Clock-Controlled Mechanisms of Glycerophospholipid Metabolism from Neuronal Cells and Tissues to Fibroblasts

Cited by 21 publications

References 233 publications

Integrated Proteomics and Metabolomics Analysis in Pregnant Rat Hippocampus After Circadian Rhythm Inversion

Integrated Proteomics and Metabolomics Analysis in Pregnant Rat Hippocampus After Circadian Rhythm Inversion

Insights about circadian clock in glioma: From molecular pathways to therapeutic drugs

Generation of Endogenous Promoter-Driven Luciferase Reporter System Using CRISPR/Cas9 for Investigating Transcriptional Regulation of the Core Clock Gene BMAL1

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