circ_SEPT9, a newly identified circular RNA, promotes oral squamous cell carcinoma progression through miR‐1225/PKN2 axis

Ai, Yilong; Tang, Zhe; Zou, Chen; Wei, Haigang; Wu, Si-Pei; Huang, Dahong

doi:10.1111/jcmm.15943

Cited by 13 publications

(8 citation statements)

References 40 publications

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“…Moreover, a recent study showed an increase in circ_0005320 in OSCC, and elevation of circ_0005320 accelerated OSCC cell survival, invasion, and migration by miR-1225/Protein Kinase N2 axis. However, large-scale identifications of circ_0005320 in the tumorigenesis of OSCC are not yet reported [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Circ_0005320 promotes oral squamous cell carcinoma tumorigenesis by sponging microRNA-486-3p and microRNA-637

Zheng

Gong

et al. 2021

Bioengineered

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Circ_0005320 was found to be elevated in oral squamous cell carcinoma (OSCC) and accelerated OSCC progression. Here, the potential mechanism of circ_0005320 in OSCC tumorigenesis was explored. The quantitative real-time polymerase chain reaction (qRT-PCR) assay was used to detect the expression of circ_0005320, miR-486-3p, and miR-637. In vitro assays were conducted using cell counting kit-8, colony formation, transwell, angiogenesis, and flow cytometry assays. The targeting relationship between microRNA (miR)-486-3p and miR-637 or circ_0005320 was confirmed using the dual-luciferase reporter and RNA immunoprecipitation (RIP) assays. The Janus Kinase 2/Signal Transducer and Activator of Transcription 3 (JAK2/STAT3) pathway-related proteins were analyzed using Western blot. The murine xenograft model was established to perform in vivo assay. Circ_0005320 expression was higher in OSCC tissues and cells. Knockdown of circ_0005320 suppressed OSCC cell growth, migration, invasion, and induced cell apoptosis in vitro, as well as impeded tumor growth in vivo. Mechanistically, miR-486-3p or miR-637 were confirmed to be a target of circ_0005320. Moreover, the inhibitory effects of circ_0005320 silencing on OSCC growth were reversed by the inhibition of miR-486-3p or miR-637. We also found that circ_0005320-miR-486-3p/miR-637 axis mediated the activation of JAK2/STAT3 pathway. This study revealed a novel regulatory network of circ_0005320-miR-486-3p/miR-637 axis in OSCC progression, suggesting that circ_0005320 might be a potential biomarker and therapeutic target for OSCC.

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Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Circ_0005320 promotes oral squamous cell carcinoma tumorigenesis by sponging microRNA-486-3p and microRNA-637

Zheng

Gong

et al. 2021

Bioengineered

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“…LB of body fluids is commonly used for the diagnosis of multiple human diseases [36,37]. Although miRNAs have been widely assessed as biomarkers in body fluids [38,39], it was recently reported that circRNAs are highly resistant to RNA degradation by exonucleases compared with miRNAs [4,9,10]. CircRNAs are also widely distributed not only within cells but in the extracellular space and various body fluids [26,40].…”

Section: Discussionmentioning

confidence: 99%

“…Jeck et al reported that putative functions of circRNA are not only miRNA sponge but also regulation of transcription, interactions with RNA binding proteins, and translation of circRNAs [8]. Among them, circRNAs have multiple microRNA (miRNA) binding sites, and circRNA binding leads to regulation of the expression of those miRNAs and their target genes by acting as a competing endogenous RNA [4,9,10]. However, the underlying molecular mechanisms and clinical correlation between circRNAs and tumor progression are not well understood.…”

Section: Introductionmentioning

confidence: 99%

Tumor Suppressive Circular RNA-102450: Development of a Novel Diagnostic Procedure for Lymph Node Metastasis from Oral Cancer

Ando

Kasamatsu

Kawasaki

et al. 2021

Cancers

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Circular RNAs (circRNAs), which form as covalently closed loop structures, have several biological functions such as regulation of cellular behavior by adsorbing microRNAs. However, there is limited information of circRNAs in oral squamous cell carcinoma (OSCC). Here, we aimed to elucidate the roles of aberrantly expressed circRNAs in OSCC. CircRNA microarray showed that circRNA-102450 was down-regulated in OSCC cells. Clinical validation of circRNA-102450 was performed using highly sensitive droplet digital PCR in preoperative liquid biopsy samples from 30 OSCC patients. Interestingly, none of 16 studied patients with high circRNA-102450 had regional lymph node metastasis (RLNM), whereas 4 of 14 studied patients (28.5%) with low expression had pathologically proven RLNM. Overexpressed circRNA-102450 significantly inhibited the tumor metastatic properties of cell proliferation, migration, and invasion. Furthermore, circRNA-102450 directly bound to, and consequently down-regulated, miR-1178 in OSCC cells. Taken together, circRNA-102450 has a tumor suppressive effect via the circRNA-102450/miR-1178 axis and may be a novel potential marker of RLNM in OSCC patients.

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“…e existent study of the function of PKN2 in tumor is relatively controversial. Particularly, PKN2 was reported as an oncogene in oral squamous cell carcinoma progression [44]. On the contrary, PKN2 was identified to inhibit tumor-associated macrophages (TAMs) polarization and tumor growth in colon cancer [45].…”

Section: Discussionmentioning

confidence: 99%

Overexpression of PAX8-AS1 Inhibits Malignant Phenotypes of Papillary Thyroid Carcinoma Cells via miR-96-5p/PKN2 Axis

Zhou

et al. 2021

International Journal of Endocrinology

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Background. Thyroid carcinoma (THCA) is the most frequent endocrine malignancy. Papillary thyroid carcinoma (PTC) is the major subtype of THCA, accounting for over 80% of all THCA cases. LncRNA PAX8-AS1, a tumor suppressor associated with various human cancers, has been reported to be relevant to the regulation of all sorts of cellular processes. The purpose of this study was to verify the role of PAX8-AS1 in PTC. Methods. Three human PTC cell lines (K1, TPC-1, and IHH4) and one normal human thyroid cell line, Nthy-ori3-1, were used in our study. The expression of genes was detected by qRT-PCR. The bioinformatic analysis and luciferase reporter assay were used to confirm the binding relationship of PAX8-AS1 to miR-96-5p, and the targeting relationship of miR-96-5p to PKN2 was also predicted. Cell proliferation and apoptosis capacities were assessed by MTT and flow cytometry, respectively. EdU assay was used to detect cell proliferation. Western blot assay was employed to examine protein expression. Results. The expression of PAX8-AS1 was decreased in PTC tissues and cells. PAX8-AS1 overexpression inhibited the proliferation of PTC cells and promoted cell apoptosis. In addition, PAX8-AS1 bonds with miR-96-5p, whose downregulation elevated the expression of PKN2 in PTC cells. Importantly, according to the rescue experiments, PKN2 silencing partially reversed the inhibitory effects of PAX8-AS1 expression on PTC cell proliferation and apoptosis. Conclusions. We found that the PAX8-AS1/miR-96-5p/PKN2 axis was closely related to the progression of PTC, which could be a potential target for treating PTC patients.

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circ_SEPT9, a newly identified circular RNA, promotes oral squamous cell carcinoma progression through miR‐1225/PKN2 axis

Cited by 13 publications

References 40 publications

RETRACTED ARTICLE: Circ_0005320 promotes oral squamous cell carcinoma tumorigenesis by sponging microRNA-486-3p and microRNA-637

RETRACTED ARTICLE: Circ_0005320 promotes oral squamous cell carcinoma tumorigenesis by sponging microRNA-486-3p and microRNA-637

Tumor Suppressive Circular RNA-102450: Development of a Novel Diagnostic Procedure for Lymph Node Metastasis from Oral Cancer

Overexpression of PAX8-AS1 Inhibits Malignant Phenotypes of Papillary Thyroid Carcinoma Cells via miR-96-5p/PKN2 Axis

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