RETRACTED ARTICLE: Circ_0005320 promotes oral squamous cell carcinoma tumorigenesis by sponging microRNA-486-3p and microRNA-637

Abstract

Circ_0005320 was found to be elevated in oral squamous cell carcinoma (OSCC) and accelerated OSCC progression. Here, the potential mechanism of circ_0005320 in OSCC tumorigenesis was explored. The quantitative real-time polymerase chain reaction (qRT-PCR) assay was used to detect the expression of circ_0005320, miR-486-3p, and miR-637. In vitro assays were conducted using cell counting kit-8, colony formation, transwell, angiogenesis, and flow cytometry assays. The targeting relationship between microRNA (miR)-486-3p and miR-637 or circ_0005320 was confirmed using the dual-luciferase reporter and RNA immunoprecipitation (RIP) assays. The Janus Kinase 2/Signal Transducer and Activator of Transcription 3 (JAK2/STAT3) pathway-related proteins were analyzed using Western blot. The murine xenograft model was established to perform in vivo assay. Circ_0005320 expression was higher in OSCC tissues and cells. Knockdown of circ_0005320 suppressed OSCC cell growth, migration, invasion, and induced cell apoptosis in vitro, as well as impeded tumor growth in vivo. Mechanistically, miR-486-3p or miR-637 were confirmed to be a target of circ_0005320. Moreover, the inhibitory effects of circ_0005320 silencing on OSCC growth were reversed by the inhibition of miR-486-3p or miR-637. We also found that circ_0005320-miR-486-3p/miR-637 axis mediated the activation of JAK2/STAT3 pathway. This study revealed a novel regulatory network of circ_0005320-miR-486-3p/miR-637 axis in OSCC progression, suggesting that circ_0005320 might be a potential biomarker and therapeutic target for OSCC.

circHIPK3 regulates cell proliferation and migration by sponging microRNA-124 and regulating serine/threonine kinase 3 expression in esophageal squamous cell carcinoma

“…For instance, circSLC7A6 can serve as a ceRNA of miR-107 to upregulate ABL2 expression that promotes the progression of Wilms’ tumor [ 8 ]. Circ_0005320 facilitates oral squamous cell carcinoma tumorigenesis via sponging miRNA-486-3p and miRNA-637 [ 9 ]. Circ_0000654 that absorbs miRNA-149-5p to upregulate inhibin-beta A enhances the growth of gastric cancer cells [ 10 ].…”

Identification of novel ceRNA networks associated with PD- L1 in head and neck squamous cell carcinoma based on whole-transcriptome sequencing

“…In the past few decades, PD-L1 has attracted more and more attention as an immunotherapy target for HNSCC. Previous studies have shown that in HNSCC, circRNAs could regulate tumor development through a variety of mechanisms, such as proliferation [32], cell cycle [33], apoptosis [34], autophagy [35], tumor angiogenesis [36], invasion [37], metastasis [38], cell stemness [39]. CircRNAs could also affect tumor progression by regulating PD-L1 [40].…”

Circ_0008068 facilitates the oral squamous cell carcinoma development by microRNA-153-3p/acylgycerol kinase (AGK) axis

“…For instance, circRNA mitochondrial translation optimization 1 homologue promoted OSCC cell proliferation, migration, and invasion in vitro via miR-320a/alpha thalassemia/mental retardation, X-linked (ATRX) axis [ 31 ]. Circ_0005320 silencing impeded OSCC growth in vivo and in vitro by sponging miR-486-3p and miR-637 [ 32 ]. Besides, hsa_circ_100533 could prevent OSCC [ 33 ].…”