circ-Sirt1 controls NF-κB activation via sequence-specific interaction and enhancement of SIRT1 expression by binding to miR-132/212 in vascular smooth muscle cells

Kong, Pengfei; Yu, Yuan; Wang, Lu; Dou, Yong-Qing; Zhang, Xuhui; Cui, Yan; Wang, Haiyue; Yong, Yu-Tao; Liu, Ya-Bin; Hu, Haijuan; Cui, Wei; Sun, Shao-Guang; Li, Binghui; Zhang, Fan; Han, Mei

doi:10.1093/nar/gkz141

Cited by 141 publications

(109 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Of note, not all circRNAs function as miRNA sponges in cells. At present, several functional models of circRNAs have been reported: (1) circRNAs function as miRNA sponges, which competitively bind endogenous miRNAs; (2) circRNAs function as “protein scaffolds,” which means that circRNAs directly bind to proteins and maintain the stability of the protein complex; 39 , 40 , 41 , 42 and (3) some circRNAs can be translated as “coding RNAs,” which means that they directly encode a protein or peptide. 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 Due to the lack of free ends, circRNAs are resistant to exonucleases and more stable than their linear isoforms.…”

Section: Discussionmentioning

confidence: 99%

ZEB1-Mediated Transcriptional Upregulation of circWWC3 Promotes Breast Cancer Progression through Activating Ras Signaling Pathway

Meng

Liu

et al. 2020

Molecular Therapy - Nucleic Acids

View full text Add to dashboard Cite

Zinc finger E-box binding homeobox 1 (ZEB1) has been widely recognized as an important driver of tumor growth and metastasis. However, nothing is known about ZEB1-regulated circular (circ)RNAs in cancer. In the current study, we evaluated the function of a novel ZEB1-regulated circRNA derived from the WWC3 gene locus, circWWC3 in breast cancer progression. We found that ZEB1 upregulated circWWC3 expression but not the linear WWC3 mRNA expression. circWWC3 is highly expressed in breast cancer tissues and is associated with the poor prognosis of breast cancer patients. Silencing of circWWC3 significantly suppresses the proliferation, migration, and invasion of breast cancer cells. Mechanically, circWWC3 upregulates multiple oncogenes’ expression of the Ras signaling pathway through acting as the sponge of microRNA (miR)-26b-3p and miR-660-3p. Moreover, short hairpin (sh)RNA-mediated knockdown of circWWC3 partially antagonized ZEB1-mediated breast cancer growth and metastasis in vivo . Our findings reveal that ZEB1-mediated upregulation of circWWC3 promotes breast cancer progression through activating Ras signaling pathway, which provides a potential therapeutic target and prognostic biomarker for breast cancer.

show abstract

Section: Discussionmentioning

confidence: 99%

ZEB1-Mediated Transcriptional Upregulation of circWWC3 Promotes Breast Cancer Progression through Activating Ras Signaling Pathway

Meng

Liu

et al. 2020

Molecular Therapy - Nucleic Acids

View full text Add to dashboard Cite

show abstract

“…2C) (50). For instance, circ-Sirt1 interacts with NF-κB p65 in the cytoplasm, thereby sequestering the nuclear translocation of p65 (induced by inflammation), and inhibiting the expression of inflammatory factors (51). circRNAs are considered to serve as protein scaffolds by harbouring binding sites for the assembly of two or more proteins, such as enzymes and their substrates, which may subsequently form large protein complexes (37).…”

Section: Functions Of Circrnasmentioning

confidence: 99%

Exosomal circular RNA sorting mechanisms and their function in promoting or inhibiting cancer (Review)

et al. 2020

View full text Add to dashboard Cite

Exosomes are nanoscale phospholipid bilayer vesicles that can be artificially engineered into vectors for the treatment of cancer. Circular RNA (circRNA), a type of non-coding RNA, has crucial regulatory functions in various aspects of cancer, such as tumorigenesis, apoptosis, proliferation, invasion, metastasis and chemo-and radiotherapeutic resistance, as well as in cancer prognosis. Notably, the exosomal transfer of circRNAs may function to both promote and inhibit cancer. Numerous studies have addressed the importance of circRNAs in cancer and non-coding RNAs (such as microRNAs and long non-coding RNAs) in exosomes. However, little research has focussed on a class of RNAs called exosomal circRNAs. The present review discusses current studies regarding exosomal circRNAs, including their biogenesis and biological functions, their abundance in exosomes and possible sorting mechanisms and their potential roles in both promoting and inhibiting cancer. It is predicted that in the next five years there will be increasing research exploring the functional mechanisms of exosomal circRNA in various diseases, in particular their roles in cancer genesis and progression.

show abstract

“…CircACTA2 competitively binds miR‐548f‐5p, which suppresses the expression of α‐SMA in VSMCs, and is involved in hypertension . Our group demonstrated that circ‐Sirt1 controls NF‐κB activation via a sequence‐specific interaction with p65 and enhancement of SIRT1 expression by sponging miR‐132/212 in the inflammatory phenotypic transformation of VSMCs . Liu et al found that the expression of circRNA‐ZNF609, a sponge for miR‐615‐5p, has a negative correlation with the velocity of vascular endothelial cell migration and tube formation and is associated with vascular endothelial dysfunction.…”

Section: Introductionmentioning

confidence: 65%

Screening and functional prediction of differentially expressed circRNAs in proliferative human aortic smooth muscle cells

Chen

Lin

et al. 2020

J Cellular Molecular Medi

View full text Add to dashboard Cite

| INTRODUC TI ONCardiovascular diseases (CVD), including vascular remodelling diseases, are the leading cause of mortality among humans throughout the world. Abnormal proliferation of vascular smooth muscle cells (VSMCs) is a common feature of many vascular remodelling diseases, including atherosclerosis, 1 hypertension 2 and vascular aneurysms. 3 The regulation of VSMC proliferation has been considered a key event due to its major implications for the prevention of pathological vascular conditions. 4 Non-coding RNAs (ncRNAs) form the dominant product of eukaryotic transcription, comprising over 73% of the human genome. 5 Circular RNAs (circRNAs) are a special novel type of endogenous ncRNAs, forming covalently closed-loop structures without 5′ caps Abstract Vascular smooth muscle cell (VSMC) proliferation is the pathological base of vascular remodelling diseases. Circular RNAs (circRNAs) are important regulators involved in various biological processes. However, the function of circRNAs in VSMC proliferation regulation remains largely unknown. This study was conducted to identify the key differentially expressed circRNAs (DEcircRNAs) and predict their functions in human aortic smooth muscle cell (HASMC) proliferation. To achieve this, DEcircRNAs between proliferative and quiescent HASMCs were detected using a microarray, followed by quantitative real-time RT-PCR validation. A DEcircRNA-miRNA-DEmRNA network was constructed, and functional annotation was performed using Gene Ontology (GO) and KEGG pathway analysis. The function of hsa_circ_0002579 in HASMC proliferation was analysed by Western blot. The functional annotation of the DEcircRNA-miRNA-DEmRNA network indicated that the four DEcircRNAs might play roles in the TGF-β receptor signalling pathway, Ras signalling pathway, AMPK signalling pathway and Wnt signalling pathway. Twenty-seven DEcircRNAs with coding potential were screened. Hsa_circ_0002579 might be a pro-proliferation factor of HASMC. Overall, our study identified the key DEcircRNAs between proliferative and quiescent HASMCs, which might provide new important clues for exploring the functions of circRNAs in vascular remodelling diseases. K E Y W O R D S circular RNAs, coding potential, HASMCs, microRNA sponge, proliferation | 4763 CHEN Et al.

show abstract

circ-Sirt1 controls NF-κB activation via sequence-specific interaction and enhancement of SIRT1 expression by binding to miR-132/212 in vascular smooth muscle cells

Cited by 141 publications

References 42 publications

ZEB1-Mediated Transcriptional Upregulation of circWWC3 Promotes Breast Cancer Progression through Activating Ras Signaling Pathway

ZEB1-Mediated Transcriptional Upregulation of circWWC3 Promotes Breast Cancer Progression through Activating Ras Signaling Pathway

Exosomal circular RNA sorting mechanisms and their function in promoting or inhibiting cancer (Review)

Screening and functional prediction of differentially expressed circRNAs in proliferative human aortic smooth muscle cells

Contact Info

Product

Resources

About