Ciprofloxacin implants for bone infection. In vitro–in vivo characterization

Castro, Carlos; Sánchez, Esther; Delgado, Araceli; Soriano, I.; Nuñez, Praxedes; Baro, Manuel; Perera, A.; Évora, Carmen

doi:10.1016/j.jconrel.2003.09.004

Cited by 86 publications

(68 citation statements)

References 29 publications

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“…On the protocol in which only half of the medium was replaced, concentration gradient was lower, explaining the slower CHX release kinetics over the 14 days incubation. The observed behavior is in agreement with several studies showing that controlled drug release profiles usually reveal an initial burst release of short duration that is followed by a longer period of continuous but declining release [4,13,18,[42][43][44], and that the release pattern is dependent on the amount of antimicrobial agent adsorbed on biomaterials [13,44].…”

Section: Discussionsupporting

confidence: 91%

“…CHX release results from desorption or partial dissolution of the CHX-phosphate binding. As expected, CHX release kinetics was clearly affected by the protocol used, because CHX is prone to leach, especially close to the discs' surface where rapid diffusion of water molecules was facilitated [13,4,42]. This process depends on the concentration gradient between the bulk solution and the disc surface.…”

Section: Discussionmentioning

confidence: 56%

“…There is a broad choice of active molecules such as anticoagulants, antiinflammatory drugs, antibiotics and extracellular matrix proteins [2]. Several authors [4][5][6][7][8] reported that the adsorption of antibiotics (e.g., gentamicin, ciprofloxacin, amoxicillin, and erythromycin) on medical devices has a huge potential in the medical field. How-ever, there is a consensus in the medical area on the rational use of antibiotics in order to avoid microbial resistance.…”

Section: Introductionmentioning

confidence: 99%

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Anti-sessile bacterial and cytocompatibility properties of CHX-loaded nanohydroxyapatite

Barros

Grenho

Fernandes

et al. 2015

Colloids and Surfaces B: Biointerfaces

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Nanohydroxyapatite possesses exceptional biocompatibility and bioactivity regarding bone cells and tis-sues, justifying its use as a coating material or as a bone substitute. Unfortunately, this feature may also encourage bacterial adhesion and biofilm formation. Surface functionalization with antimicrobials is a promising strategy to reduce the likelihood of bacterial infestation and colonization on medical devices. Chlorhexidine digluconate is a common and effective antimicrobial agent used for a wide range of medical applications. The purpose of this work was the development of a nanoHA biomaterial loaded with CHX to prevent surface bacterial accumulation and, simultaneously, with good cytocompatibility, for application in the medical field. CHX (5-1500 mg/L) was loaded onto nanoHA discs and the materials were evaluated for CHX adsorption and release profile, physic-chemical features, antibacterial activity against Escherichia coli, Staphylococcus aureus and Staphylococcus epidermidis, and cytocompatibility toward L929 fibroblasts. Results showed that the adsorption of CHX on nanoHA surface occurred by electrostatic inter-actions between the cationic group of CHX and the phosphate group of nanoHA. The release of CHX from CHX-loaded nanoHA showed a fast initial rate followed by a slower kinetics release, due to constraints caused by dilution and diffusion-limiting processes. NanoHA.50 to nanoHA.1500 showed strong anti-sessile activity, inhibiting bacterial adhesion and the biofilm formation. CHX-nanoHA caused a dose-and time-dependent inhibitory effect on the proliferation of fibroblasts for nanoHA.100 to nanoHA.1500. Cellular behavior on 2 nanoHA.5 and nanoHA.50 was similar to control. Therefore, CHX-loaded nanoHA surfaces appear as a promising alternative to prevention of devices-related infections.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 56%

Section: Introductionmentioning

confidence: 99%

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Anti-sessile bacterial and cytocompatibility properties of CHX-loaded nanohydroxyapatite

Barros

Grenho

Fernandes

et al. 2015

Colloids and Surfaces B: Biointerfaces

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“…PMB release in vitro began after 2 -3 days of incubation for blocks compacted at 25 m s 21 velocity and on day 5 for those compacted at 50 m s 21 (Kimakhe et al 1999). Some important results have shown the potential effectiveness of quinolones in local DDS designed to treat bone infection using carriers such as HA -anionic collagen composite (Martins & Goissis 2000), biodegradable polymers (Overbeck et al 1995;Nicolau et al 1998;Nie et al 1998;Ramchandani & Robinson 1998;Kanellakopoulou & Giamarellos-Bourboulis 2000;Désévaux et al 2002) and mixtures of calcium phosphates and biodegradable polymers (Castro et al 2003). A composite of gatifloxacine (GFLX)-loaded poly-x-caprolactone combined with x-TCP porous ceramic was obtained by compression moulding, followed by sintering (Miyai et al 2008).…”

mentioning

confidence: 99%

Bone tissue engineering therapeutics: controlled drug delivery in three-dimensional scaffolds

Mouriño

Boccaccını

2009

J. R. Soc. Interface.

471

298

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This paper provides an extensive overview of published studies on the development and applications of three-dimensional bone tissue engineering (TE) scaffolds with potential capability for the controlled delivery of therapeutic drugs. Typical drugs considered include gentamicin and other antibiotics generally used to combat osteomyelitis, as well as anti-inflammatory drugs and bisphosphonates, but delivery of growth factors is not covered in this review. In each case reviewed, special attention has been given to the technology used for controlling the release of the loaded drugs. The possibility of designing multifunctional three-dimensional bone TE scaffolds for the emerging field of bone TE therapeutics is discussed. A detailed summary of drugs included in three-dimensional scaffolds and the several approaches developed to combine bioceramics with various polymeric biomaterials in composites for drug-delivery systems is included. The main results presented in the literature are discussed and the remaining challenges in the field are summarized with suggestions for future research directions.

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“…Castro et al prepared composite by simple mixing and compression (57). PLA, phosphates (Hap/TCP), and drug were mixed and pressed in carver hydraulic press at 520 or 312 MPa for 5 min.…”

Section: Compressionmentioning

confidence: 99%

Organic–Inorganic Composites for Bone Drug Delivery

Soundrapandian

Datta

2009

AAPS PharmSciTech

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Abstract. This review paper attempts to provide an overview in the fabrication and application of organic-inorganic based composites in the field of local drug delivery for bone. The concept of local drug delivery exists for a few decades. However, local drug delivery in bone and specially application of composites for delivery of drugs to bone is an area for potential research interest in the recent time. The advantages attained by an organic-inorganic composite when compared to its individual components include their ability to release drug, adopting to the natural environment and supporting local area until complete bone regeneration, which make them carriers of interest for local drug delivery for bone.

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Ciprofloxacin implants for bone infection. In vitro–in vivo characterization

Cited by 86 publications

References 29 publications

Anti-sessile bacterial and cytocompatibility properties of CHX-loaded nanohydroxyapatite

Anti-sessile bacterial and cytocompatibility properties of CHX-loaded nanohydroxyapatite

Bone tissue engineering therapeutics: controlled drug delivery in three-dimensional scaffolds

Organic–Inorganic Composites for Bone Drug Delivery

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