Cicatricial pemphigoid sera specifically react with the most C-terminal portion of BP180

Salivary IgA and IgG antibodies to bullous pemphigoid 180 noncollagenous domain 16a as diagnostic biomarkers in mucous membrane pemphigoid

“…The NC16a domain of BP180 is targeted by IgG and/or IgA autoantibodies in up to 50% of patients with MMP . In addition, other studies report reactivity against the C‐terminal domain of BP180 . Although no clear association between target antigens and a specific clinical phenotypes has been reported, some studies have suggested an association between ocular pemphigoid and the β4 integrin, while a possible association between oral disease and α6 integrin has been proposed …”

A systematic review with pooled analysis of clinical presentation and immunodiagnostic testing in mucous membrane pemphigoid: association of anti‐laminin‐332 IgG with oropharyngeal involvement and the usefulness of ELISA

“…The particular ELISA technique is quite important, as the C‐terminal domain appears to have a more significant role in MMP than the NC16a . IgG reactivity with both the N‐ and C‐terminal domains is associated with mucosal lesions in BP patients .…”

Detection of pemphigus desmoglein 1 and desmoglein 3 autoantibodies and pemphigoid BP180 autoantibodies in saliva and comparison with serum values

“…The recent availability of cDNA clones for pemphigus and pemphigoid antigens has led to the production of recombinant proteins representing epitopes of native antigens Dsg1, Dsg3, BP180 and BP230, and to the development of related enzyme-linked immunosorbent assay (ELISA) systems (21)(22)(23)(24)(25). The advantages of these assays are their greater sensitivity and specificity with respect to other serological tests (20,26).…”