Chrysin-induced apoptosis is mediated through caspase activation and Akt inactivation in U937 leukemia cells

Woo, Kyung Jin; Jeong, Yong-Jin; Park, Jong‐Wook; Kwon, Taeg Kyu

doi:10.1016/j.bbrc.2004.09.225

Cited by 155 publications

(88 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Chrysin has been demonstrated to possess cancer chemopreventive activity through inhibition of cell proliferation (10,18), and induce apoptosis in various malignant cancer cells (5,11,(15)(16)(17)(20)(21)(22).…”

Section: Discussionmentioning

confidence: 99%

“…Chrysin was initially identified for its anti-oxidant effects and has been shown to possess cancer chemopreventive and anti-tumor effects (6)(7)(8). It inhibits proliferation and induces apoptosis in a variety of cancer cells in vitro, including cells from the prostate, skin, breast, lung, cervix, thyroid cancer and leukemia (5,(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22). However, to the best of our knowledge, the effects of chrysin on uveal melanoma cells have not been previously studied.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Chrysin induces cell apoptosis in human uveal melanoma cells via intrinsic apoptosis

Xue

Chen

et al. 2016

Oncology Letters

View full text Add to dashboard Cite

Abstract. Uveal melanoma is the most common intraocular malignant tumor in adults. Chrysin is a flavonoid present in honey, propolis, various plants and herbs. In the present study, the cytotoxic effects of chrysin were investigated on human uveal melanoma cell lines (M17 and SP6.5) and associated signaling pathways, and a comparison to the effects on normal ocular cells [scleral fibroblasts and retinal pigment epithelial (RPE) cells] was performed. The effects of chrysin on cell viability were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Cell apoptosis was determined by using terminal deoxynucleotidyl transferase mediated dUTP nick end-labeling assay. Mitochondrial permeability was determined by JC-1 fluorescein analysis. Cytosol cytochrome c levels, and the activities of caspase-3, -8 and -9 were measured by enzyme-linked immunosorbent assay or colorimetric assay. Chrysin reduced the viability of cultured human melanoma cells in a dose-dependent manner (0, 10, 30 and 100 µM) with IC 50 at 28.3 and 35.8 µM in SP6.5 and M17 cell lines, respectively. Chrysin at 30-100 µM levels selectively reduced the viability of melanoma cells without affecting the viability of scleral fibroblasts and RPE cells. Chrysin increased mitochondrial permeability, the levels of cytosol cytochrome c, and caspase-9 and -3 activities, but not capase-8 activity in uveal melanoma cells. The results of the present study indicate that chrysin induces apoptosis of human uveal melanoma cells via the mitochondrial signaling pathway and suggest that chrysin may be a promising agent in the treatment of uveal melanoma.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Chrysin induces cell apoptosis in human uveal melanoma cells via intrinsic apoptosis

Xue

Chen

et al. 2016

Oncology Letters

View full text Add to dashboard Cite

show abstract

“…Woo et al [18] also used a different component of propolis, chrysin in U937 cells and they showed that chrysin activated caspase-3 and inactivated Akt [18]. Propolin A, propolin B and propolin C, components of propolis, were used by Chen et al [19,20] in human melanoma A2058, HL-60, MCF-7, human neuroblastoma IMR-32, C6, murine melanoma B16F10, Hep.G2, human hepatocellular carcinoma Hep.3B and HT-29 cells.…”

Section: Discussionmentioning

confidence: 99%

Effect of Turkish pollen and propolis extracts on caspase-3 activity in myeloid cancer cell lines

Uçar¹,

Gerigelmez²,

Cengiz³

et al. 2016

Trop. J. Pharm Res

View full text Add to dashboard Cite

“…Other plants extracts that were previously studied as anti-cancer agents include chrysin which is also a naturally occurring flavone commonly found in blue passion flower, and tannic acid which is widely found in food plants. Chrysin is able to induce apoptosis and possess anti-proliferative effects on various leukemias [5,6] demonstrating direct intervention of key signaling pathways controlling these cellular functions. Good anti-oxidants such as apigenin have also been studied for its effects in reducing geno-toxic damage of drug treatments for leukemia [7].…”

Section: Introductionmentioning

confidence: 99%

Identifying Combinatorial Growth Inhibitory Effects of Various Plant Extracts on Leukemia Cells through Systematic Experimental Design

Cheong¹,

Htay.²,

Tan³

et al. 2012

AJPS

View full text Add to dashboard Cite

Plant extracts are widely studied for their anti-cancer and cancer preventive effects. In this study, we compared the leukemia growth inhibition effects of seven different plant extracts, theaflavin, epigallocatechin gallate (EGCG), epicathechin (EC), apigenin, quercetin, chrysin and tannic acid, in vitro using the K562 erythroleukemia cell line and application of the design of experiments (DoE) methodology. Our systematic approach enabled us to isolate the main factor contribution, two-factor interactions and produced interaction relationships and/or models to describe growth inhibitory effects of different plant extracts when they are used in combination. The results identified tannic acid as the most significant inhibitor in this group and had synergistic effects with EGCG at specific concentrations. The fitted model of their combined effects showed that the most potent combination is at low concentrations of tannic acid (10 -20 µM) and high concentrations of EGCG (80 -100 µM). We further showed that tannic acid induced both growth inhibition and apoptosis in K562 cells in ranges between 10 -100 µM. The polyphenol caused cell cycle arrest at G2-phase under the higher concentrations. In summary, use of DoE techniques effectively identified the most prominent inducer in this group of plant bioactive compounds and produced combinatorial bioactivity of various polyphenols and flavonoids over the entire range of concentrations under study. This study exemplifies the usefulness of DoE and serves as a guide in its utility for in vitro assessment of bioactivity in plant constituents.

show abstract

Chrysin-induced apoptosis is mediated through caspase activation and Akt inactivation in U937 leukemia cells

Cited by 155 publications

References 30 publications

Chrysin induces cell apoptosis in human uveal melanoma cells via intrinsic apoptosis

Chrysin induces cell apoptosis in human uveal melanoma cells via intrinsic apoptosis

Effect of Turkish pollen and propolis extracts on caspase-3 activity in myeloid cancer cell lines

Identifying Combinatorial Growth Inhibitory Effects of Various Plant Extracts on Leukemia Cells through Systematic Experimental Design

Contact Info

Product

Resources

About