Chronic unpredictable stress disturbs the blood–testis barrier affecting sperm parameters in mice

Kolbaşı, Bircan; Bülbül, Muhammet Volkan; Karabulut, Seda; Altun, Ceren Erdem; Çakıcı, Çağrı; Ülfer, Gözde; Müdok, Tangül; Keskin, İlknur

doi:10.1016/j.rbmo.2020.12.007

Cited by 19 publications

(14 citation statements)

References 57 publications

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“…The strain of C57BL/6 mice was chosen for study due to their behavioral profile, such as moderate to high levels of social pattern, reversal learning and exploration behavior ( Moy et al, 2007 ). In recent years, C57BL/6 mice have been widely used in behavioral studies ( An et al, 2011 ; Kubera et al, 1998 ; Yao et al, 2016 ), and used as research objects for depression to explore the changes of biochemical molecule levels ( Kolbasi et al, 2021 ; Li & Singh, 2014 ). A few research displayed some sex differences in behavior and hormone secretion but lack of comprehensive biochemical molecules profile elaboration ( Dalla et al, 2011 ; Goodwill et al, 2019 ; Huynh et al, 2011 ; Labaka et al, 2018 ; Leussis et al, 2012 ; Liu et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

“…Even in the same age group the incidence of depression in females was higher than that in males ( Barnett et al, 2021 ; Euteneuer et al, 2011 ; Seifert et al, 2021 ). Females seemed to be more susceptible to stress via the hypothalamus pituitary adrenal (HPA) axis and leading to abnormal hormone secretion ( Blier & El Mansari, 2013 ; Kolbasi et al, 2021 ; Mainio et al, 2006 ; Zefferino, Di Gioia & Conese, 2021 ). In addition, from the perspective of social psychology, females were more challenged by job and family stress which might aggravate depressive disorder ( Isaksson et al, 2020 ; Maji, 2018 ; Wang et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

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Sex differences in peripheral monoamine transmitter and related hormone levels in chronic stress mice with a depression-like phenotype

Chen

Cai

et al. 2022

PeerJ

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Backgrounds Chronic stress could induce depression-like phenotype in animal models. Previous data showed that sex differences exist after chronic stress model establishment, however, the detailed information about the difference of blood biochemical indexes is not clear. In this study, we aim to supply comparison of monoamine transmitters and related hormone markers in serum between male and female depressed mice, and in order to better understand the sex difference in transmitters and hormone levels in depression occurrence and development. Methods Sixty C57BL/6 mice (both male and female) were divided into two groups by gender. Same gender mice were then divided randomly into the non-treated control group and chronic stress group which was exposed to 8 weeks of chronic unpredictable mild stress (CUMS). Depression-like behavior was assessed with open-field test and sucrose preference test. Blood sample was collected and monoamine transmitter and related hormone in serum were measured by ELISA. Results The depression-like phenotype mice model was established successfully after 8 weeks of chronic stress. The locomotion activity scores in male stressed mice declined more than that in female stressed mice, while the exploratory behavior scores in female stressed mice declined more than that in male stressed mice. Compared to non-treated control group mice, mice in the chronic stress group in response to stress showed greater declines in monoamine transmitters (5-HT, dopamine, norepinephrine) and sex hormones (androgen, estrogen, oxytocin and prolactin), while stress hormones (adrenaline, corticosterone and ACTH) were significantly increased. The decrease of norepinephrine, androgen and estrogen in female stressed mice was greater than in male stressed mice, whereas the 5-HT and oxytocin in male stressed mice decreased more than in female stressed mice, and the corticosterone in male stressed mice increased more than in female stressed mice. Conclusion Sex differences of monoamine transmitter and related hormone levels in serum occurred in chronic stress induced depression-like phenotype mice model. It may provide a useful reference to guide precise antidepressant treatment in different gender population in clinical care.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Sex differences in peripheral monoamine transmitter and related hormone levels in chronic stress mice with a depression-like phenotype

Chen

Cai

et al. 2022

PeerJ

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“…Psychological stress may have a significant impact on spermatogenesis since suppression of the hypothalamic-pituitary-gonadal axis produces a drop in testosterone levels, which causes alterations in Sertoli cells and the BTB, resulting in spermatogenesis arrest ( Morgan et al, 2014 ; Fan et al, 2015 ). Chronic stress has been shown to impact critical proteins and sperm characteristics of the BTB, including BTB and ZO-1 integrity as well as CLDN11 levels ( Kolbasi et al, 2021 ). Sleep deprivation reduces the expression of TJ proteins, actin, and androgen receptor as well as sperm viability and motility as a result of blood-testis changes ( Dominguez-Salazar et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Bibliometric and visual analysis of blood-testis barrier research

et al. 2022

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Background: Extensive research on the blood-testis barrier has been undertaken in recent years. However, no systematic bibliometric study has been conducted on this subject. Our research aimed to identify the hotspots and frontiers of blood-testis barrier research and to serve as a guide for future scientific research and decision-making in the field.Methods: Studies on the blood-testis barrier were found in the Web of Science Core Collection. VOSviewer, CiteSpace, and Microsoft Excel were used to conduct the bibliometric and visual analyses.Results: We found 942 blood-testis barrier studies published in English between 1992 and 2022. The number of annual publications and citations increased significantly between 2011 and 2022, notably in the United States. China and the United States, the US Population Council, Endocrinology, and Cheng C. Yan were the most productive countries, institution, journal, and author, respectively. The study keywords indicated that blood-testis barrier research involves a variety of compositional features (tight junctions, cytoskeleton, adherens junctions), cell types (Sertoli cells, germ cells, Leydig cells, stem cells), reproductive toxicity (cadmium, nanoparticles, bisphenol-a), and relevant mechanisms (spermatogenesis, apoptosis, oxidative stress, dynamics, inflammation, immune privilege).Conclusion: The composition and molecular processes of the blood-testis barrier as well as the blood-testis barrier in male infertility patients are the primary research hotspots in this field. In addition, future research will likely focus on treatment and the development of novel medications that target signal pathways in oxidative stress and apoptosis to preserve the blood-testis barrier. Further studies must extend to clinical diagnosis and therapy.

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“…The increase in abnormal sperm rate may be a consequence of a disrupted BTB structure [41]. Therefore, we detected whether miR-181c/d could affect the BTB function in vivo.…”

Section: Mir-181c/d Delivery In Murine Testes Reduces Sertoli Cell Nu...mentioning

confidence: 98%

Sertoli cell survival and barrier function are regulated by miR-181c/d-Pafah1b1 axis during mammalian spermatogenesis

Feng

Chen

Wang

et al. 2022

Preprint

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Sertoli cells contribute to the formation of the blood-testis barrier (BTB), which is necessary for normal spermatogenesis. Recently, microRNAs (miRNAs) have emerged as posttranscriptional regulatory elements in BTB function during spermatogenesis. Our previous study has shown that miR-181c or miR-181d (miR-181c/d) is highly expressed in testes from boars at 60 days old compared with at 180 days old. Herein, we found that overexpression of miR-181c/d via miR-181c/d mimics in murine Sertoli cells (SCs) or miR-181c/d-overexpressing lentivirus in murine testes perturbs BTB function by altering BTB-associated protein distribution at the Sertoli cell-cell interface and F-actin organization, but this in vivo perturbation disappears approximately six weeks after the final treatment. We also found that miR-181c/d represses Sertoli cell proliferation and promotes its apoptosis. Moreover, miR-181c/d regulates Sertoli cell survival and barrier function by targeting platelet-activating factor acetylhydrolase 1b regulatory subunit 1 (Pafah1b1) gene. Furthermore, miR-181c/d suppresses PAFAH1B1 expression, reduces the complex of PAFAH1B1 with IQ motif-containing GTPase activating protein 1, and inhibits CDC42/PAK1/LIMK1/Cofilin pathway which is required for F-actin stabilization. In total, our results reveal the regulatory axis of miR-181c/d-Pafah1b1 in cell survival and barrier function of Sertoli cells, and provide additional insights into miRNA functions on the spermatogenesis process in mammals.

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Chronic unpredictable stress disturbs the blood–testis barrier affecting sperm parameters in mice

Cited by 19 publications

References 57 publications

Sex differences in peripheral monoamine transmitter and related hormone levels in chronic stress mice with a depression-like phenotype

Sex differences in peripheral monoamine transmitter and related hormone levels in chronic stress mice with a depression-like phenotype

Bibliometric and visual analysis of blood-testis barrier research

Sertoli cell survival and barrier function are regulated by miR-181c/d-Pafah1b1 axis during mammalian spermatogenesis

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