Chronic heat stress increases insulin-like growth factor-1(IGF-1) but does not affect IGF-binding proteins in growing pigs

Xin, Hairui; Zhang, Xiaojun; Sun, Dengsheng; Zhang, Chuang; Hao, Ya; Gu, Xianhong

doi:10.1016/j.jtherbio.2018.08.008

Cited by 19 publications

(10 citation statements)

References 57 publications

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“…The rise of LA caused by glycolysis was also observed (Baumgard and Rhoads ). Moreover, the ascension of IGF‐1 level indicated the acceleration of glucose metabolism (Xin et al ). Stress can activate the hypothalamic–pituitary–adrenal (HPA) axis to release stress hormones such as ACTH, cortisol and adrenaline (Gou et al ; Yu et al ).…”

Section: Discussionmentioning

confidence: 99%

Effects of acute heat stress on intestinal microbiota in grow‐finishing pigs, and associations with feed intake and serum profile

Xiong

et al. 2019

J Appl Microbiol

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Aims This study was conducted to assess the effects of acute heat stress (HS) on intestinal microbiota, and the associations with the changes in feed intake (FI) and serum profile. Methods and Results Twenty four individually housed pigs (Duroc × Large White × Landrace, 30 ± 1 kg body weight) were randomly assigned to receive one of three treatments (8 pigs/treatment): (i) thermal neutral (TN) conditions (25 ± 1°C), (ii) HS conditions (35 ± 1°C), (iii) pair‐feeding (PF) with HS under TN conditions. After 24‐h treatment, pigs were monitored to assess FI, and samples of serum and faeces were collected to investigate serum profile, microbial composition and short chain fatty acids (SCFAs). The results showed that HS decreased (P < 0·05) FI compared with the TN group. Compared with TN group, HS changed the serum profile by affecting biochemical parameters and hormones related with energy metabolism and stress response; immune indicators were also altered in HS group. Most of changes in serum profile were independent of FI reduction. Additionally, HS shifted the diversity and composition of faecal microbial community by increasing (P < 0·05) Proteobacteria and decreasing (P < 0·05) Bacteroidetes. Moreover, HS decreased (P < 0·05) the concentrations of propionate, butyrate, valerate, iso‐valerate and total SCFAs in faeces in an FI‐independent manner. Furthermore, the Spearman correlation analysis implied that changes of serum profile have potential correlation with alterations of faecal microbiota and their SCFAs metabolites in acute HS‐treated grow‐finishing pigs. Conclusions Metabolism disorders caused by 24‐h acute HS associated with changes of faecal microbiota and their SCFAs metabolites in an FI‐independent manner in grow‐finishing pigs. Significance and Impact of the Study These results give us a new insight of the intestinal damage caused by acute HS and the underlying mechanisms.

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Section: Discussionmentioning

confidence: 99%

Effects of acute heat stress on intestinal microbiota in grow‐finishing pigs, and associations with feed intake and serum profile

Xiong

et al. 2019

J Appl Microbiol

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“…The thermocycler (T100TM Thermal Cycler, Bio-Rad, Hercules, CA, USA) was used to synthesize the complimentary DNA and a CFX96 real-time PCR detection system (Bio-Rad, Hercules, CA, USA) was used for real-time quantitative PCR (qPCR). To obtain the mRNA abundance of target and housekeeping (β-actin) [ 58 ] genes, specific primers ( Supplementary Table S3 ) for following genes were obtained from previous studies or designed in-house: MUC1 [ 59 ], mucin 2 ( MUC2 ) [ 59 ], EGFR [ 60 ], insulin-like growth factor-1 receptor ( IGF-1R ) [ 61 ], caspase-9 [ 62 ], Ki-67 [ 63 ], PPARγ [ 64 ], IGFBP1 [ 65 ], IGF-1 [ 66 ], mTOR [ 67 ], ATF4 [ 68 ], CSNK2A1 [ 69 ], OCLN [ 70 ], ZO1 [ 70 ], PRKCA , and GCN2 . The qPCR program was followed by a melt curve program.…”

Section: Methodsmentioning

confidence: 99%

A Mixture of Valine and Isoleucine Restores the Growth of Protein-Restricted Pigs Likely through Improved Gut Development, Hepatic IGF-1 Pathway, and Plasma Metabolomic Profile

Habibi

Goodarzi

Shili

et al. 2022

IJMS

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Valine (Val) alone or in combination with isoleucine (Ile) improves the growth under severe protein restriction; however, the underlying mechanisms remain unknown. In this study, we assessed whether Val/Ile-induced growth in protein-restricted pigs is associated with changes in gut development, hepatic insulin-like growth factor 1 (IGF-1) production, and blood metabolomics. Forty piglets were assigned to five dietary groups: positive control (PC) with standard protein content; low protein (LP) with very low protein content; and LP supplemented with Val (LPV), Ile (LPI), and Val and Ile (LPVI). LPVI reversed the negative effects of VLP diets on growth and gut morphology. Both LPV and LPVI restored the reduced transcript of IGF-1 while decreasing the transcript of insulin-like growth factor binding protein 1 (IGFBP1) in the liver. LPV and LPVI recovered the reduced plasma Val, glycine, and leucine concentrations, which were positively correlated with improved gut morphology and the hepatic IGF-1 gene expression and negatively correlated with hepatic IGFBP1 mRNA abundance. In conclusion, supplementation with a combination of Val and Ile into the VLP diets restored the decreased growth performance of pigs fed with these diets likely through improved gut development, hepatic IGF-1 expression and bioavailability, and plasma metabolomics profile.

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“…Insulin-like growth factor-binding protein 3 (IGFBP-3) plays a critical role in the physiological function of insulin growth factor 1 (IGF-1) [ 31 ]. The expressed gene is known as the main circulating carrier of IGF-1 and the high expression in this study indicates the over-activation of the IGF-1 protein.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Acute and Chronic Thermotherapy on Type 2 Diabetic Skeletal Muscle Gene Expression and Inflammatory Markers

et al. 2021

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Background: Type 2 diabetes (T2D) is a chronic illness associated with resistance to or defective insulin secretion. This study investigates the effects of thermotherapy on cell viability, gene expression and inflammation in skeletal muscle cell lines. Methods: Healthy and T2D human skeletal muscle cell lines (HSMM and D-HSMM, respectively) were subjected to acute or chronic thermo-therapy (AT or CT, respectively). CT consisted of a 30 min exposure to 40 °C, three times a week for three weeks; AT was a one-time exposure. Results: A significant decrease in D-HSMM cell viability percentage followed AT; however, no significant change occurred in CT. HSMM yielded the highest elevations of genes following CT. In D-HSMM, both treatments yielded gene upregulation. Both treatments significantly down-regulated IL-1β, IL-6, IL-10 and TNF-α in HSMM. AT significantly decreased IL-1β, IL-6 and upregulated IL-10 and TNF-α levels in D-HSMM, while CT yielded a reduction in IL-4, TNF-α and an upregulation of IL-6 and IL-10. Conclusions: An increase in gene expression indicates actin activity and cellular responses, suggesting an increase in transcriptional regulation. The upregulation of IL-6 and IL-10 in D-HSMM negatively correlated with a decrease in TNF-α and IL-1β, indicating improved adverse inflammatory effects associated with the disease.

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Chronic heat stress increases insulin-like growth factor-1(IGF-1) but does not affect IGF-binding proteins in growing pigs

Cited by 19 publications

References 57 publications

Effects of acute heat stress on intestinal microbiota in grow‐finishing pigs, and associations with feed intake and serum profile

Effects of acute heat stress on intestinal microbiota in grow‐finishing pigs, and associations with feed intake and serum profile

A Mixture of Valine and Isoleucine Restores the Growth of Protein-Restricted Pigs Likely through Improved Gut Development, Hepatic IGF-1 Pathway, and Plasma Metabolomic Profile

The Effect of Acute and Chronic Thermotherapy on Type 2 Diabetic Skeletal Muscle Gene Expression and Inflammatory Markers

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