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Valine (Val) alone or in combination with isoleucine (Ile) improves the growth under severe protein restriction; however, the underlying mechanisms remain unknown. In this study, we assessed whether Val/Ile-induced growth in protein-restricted pigs is associated with changes in gut development, hepatic insulin-like growth factor 1 (IGF-1) production, and blood metabolomics. Forty piglets were assigned to five dietary groups: positive control (PC) with standard protein content; low protein (LP) with very low protein content; and LP supplemented with Val (LPV), Ile (LPI), and Val and Ile (LPVI). LPVI reversed the negative effects of VLP diets on growth and gut morphology. Both LPV and LPVI restored the reduced transcript of IGF-1 while decreasing the transcript of insulin-like growth factor binding protein 1 (IGFBP1) in the liver. LPV and LPVI recovered the reduced plasma Val, glycine, and leucine concentrations, which were positively correlated with improved gut morphology and the hepatic IGF-1 gene expression and negatively correlated with hepatic IGFBP1 mRNA abundance. In conclusion, supplementation with a combination of Val and Ile into the VLP diets restored the decreased growth performance of pigs fed with these diets likely through improved gut development, hepatic IGF-1 expression and bioavailability, and plasma metabolomics profile.

Low birthweight (LBW) is associated with metabolic complications, such as glucose and lipid metabolism disturbances in early life. The objective of this study was to assess: (1) the effect of dietary tryptophan (Trp) on glucose and fat metabolism in an LBW piglet model, and (2) the role peripheral 5-hydroxytryptamine type 3 (5HT3) receptors in regulating the feeding behavior in LBW piglets fed with Trp-supplemented diets. Seven-day-old piglets were assigned to 4 treatments: normal birthweight-0%Trp (NBW-T0), LBW-0%Trp (LBW-T0), LBW-0.4%Trp (LBW-T0.4), and LBW-0.8%Trp (LBW-T0.8) for 3 weeks. Compared to LBW-T0, the blood glucose was decreased in LBW-T0.8 at 60 min following the meal test, and the triglycerides were lower in LBW-T0.4 and LBW-T0.8. Relative to LBW-T0, LBW-T0.8 had a lower transcript and protein abundance of hepatic glucose transporter-2, a higher mRNA abundance of glucokinase, and a lower transcript of phosphoenolpyruvate carboxykinase. LBW-T0.4 tended to have a lower protein abundance of sodium-glucose co-transporter 1 in the jejunum. In comparison with LBW-T0, LBW-T0.4 and LBW-T0.8 had a lower transcript of hepatic acetyl-CoA carboxylase, and LBW-T0.4 had a higher transcript of 3-hydroxyacyl-CoA dehydrogenase. Blocking 5-HT3 receptors with ondansetron reduced the feed intake in all groups, with a transient effect on LBW-T0, but more persistent effect on LBW-T0.8 and NBW-T0. In conclusion, Trp supplementation reduced the hepatic lipogenesis and gluconeogenesis, but increased the glycolysis in LBW piglets. Peripheral serotonin is likely involved in the regulation of feeding behavior, particularly in LBW piglets fed diets supplemented with a higher dose of Trp.

This study was carried out to determine whether dietary fish oil (FO) with or without vitamin E (VE) supplementation would have a beneficial effect on characteristics of fresh and cryopreserved ovine sperm. Sixteen rams were allotted to four groups and fed one of the four diets: control diet (CON; without FO and VE supplementation), VE diet supplying 200 IU VE/day.ram, FO diet containing 2.5% (dry matter basis) FO, and OVE diet containing FO and VE. Semen samples were collected at Weeks 7, 9 and 11 for evaluation of seminal quality. Frozen samples from Weeks 9 and 11 were subjected to computer-assisted semen analysis (CASA). Seminal volume, percentage of sperm with normal morphology and intact acrosome and seminal concentration of malondialdehyde were increased in FO rams (P < 0.05). In VE rams, semen volume and percentage of morphologically normal sperm were higher compared with CON rams; however, in OVE rams these attributes were not different from those in CON rams. Other traits in fresh semen were not affected by the diet. Fish oil alone negatively affected the sperm viability, but not motility, of the frozen–thawed semen; however, simultaneous supplementation with FO and VE resulted in increased percentage of morphologically normal sperm, motility, and viability. The percentages of CASA type A (showing rapid progressive motility) and type B (showing medium progressive motility) sperm were higher in OVE compared with other groups. Vitamin E supplementation decreased the beat-cross frequency values compared with other groups. Other CASA parameters were not affected by the diets. In conclusion, dietary FO and VE in the breeding season of ram, generally, do not cause significant changes in fresh sperm quality but, for cryopreservation of sperm, supplementation of the diet with an antioxidant such as VE may be beneficial when polyunsaturated fatty acids are added to the diet.

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