Branched-chain amino acids partially recover the reduced growth of pigs fed with protein-restricted diets through both central and peripheral factors

Habibi, Mohammad; Shili, Cedrick N; Sutton, Julia; Goodarzi, Parniyan; Maylem, Excel Rio S.; Spicer, L. J.; Pezeshki, Adel

doi:10.1016/j.aninu.2021.02.002

Cited by 13 publications

(52 citation statements)

References 76 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Milk replacer powder was formulated for piglets (Supplemental Table S1), according to the nutrient requirements suggested by previous publications for neonatal pigs [36,37]. For the diet formulations, the chemical composition of the analyzed ingredients in this study (i.e., Arg, His, Phe, whey powder, and whey protein concentrate; Supplemental Tables S2 and S3) and our previous study was used [38]. Except for L-Trp, the amount of ingredients used across all three diets were kept consistent, and all diets were prepared as isonitrogenous and isocaloric by adding L-alanine and dextrose, respectively.…”

Section: Experimental Design and Dietsmentioning

confidence: 99%

“…Following the blood collection at 120 min after the meal test, all pigs were euthanized using the CO 2 asphyxiation method. As previously described [38], the hypothalamus, duodenum, jejunum, white adipose tissue, and liver were collected immediately after euthanasia, flushed with distilled water, snap-frozen in liquid nitrogen, and stored at −80 • C for further analyses.…”

Section: Meal Test and Blood And Tissue Collectionmentioning

confidence: 99%

“…The dry matter, crude protein, crude fiber, calcium, and phosphorus of the diets and some ingredients contents (i.e., Arg, His, Phe, and whey powder) were analyzed by Servi-Tech laboratory (Dodge City, KS, USA) (Supplemental Table S2), as previously described [38,[42][43][44]. The complete amino acids profile of the experimental diets and whey protein concentrate were analyzed [45] by Agricultural Experiment Station Chemical Laboratories (University of Missouri-Columbia, MO, USA) (Supplemental Tables S3 and S4).…”

Section: Diet Proximate and Amino Acids Analysismentioning

confidence: 99%

“…RNA isolation and RT-qPCR were performed for glucose transporter 1 (GLUT1), glucokinase (GCK), liver-type phosphofructokinase (PFKL), liver-type pyruvate kinase (PKLR), pyruvate carboxylase (PC), phosphoenolpyruvate carboxykinase (PEPCK), glucose-6-phosphatase (G6PC), glucose transporter 2 (GLUT2), lipoprotein lipase (LPL), cluster of differentiation 36 molecule (CD36), fatty acid synthase (FAS), acetyl-CoA carboxylase (ACC), hydroxyacyl-CoA dehydrogenase (HADH), sterol regulatory element binding transcription factor 1 (SREBP-1), peroxisome proliferator activated receptor alpha (PPARα), and PPARG coactivator 1 alpha (PGC1α) in the liver, following our published procedures [38,49,50]. The quality of isolated RNA was measured by a NanoDrop ND-1000 spectrophotometer (Thermo Fisher, Waltham, MA, USA).…”

Section: Rna Isolation and Rt-qpcrmentioning

confidence: 99%

“…The quality of isolated RNA was measured by a NanoDrop ND-1000 spectrophotometer (Thermo Fisher, Waltham, MA, USA). The complementary cDNA was synthesized by a thermoscycler (T100TM Thermal Cycler, Bio-Rad, Hercules, CA, USA), and then a CFX96 real-time PCR detection system (Bio-Rad, Hercules, CA, USA) was used to measure the mRNA abundance of the target genes through real-time quantitative PCR (qPCR), followed by a melt curve, as previously described [38,49,50]. The primers used are shown in Supplemental Table S5 and were obtained from previous publications [51][52][53][54][55][56][57][58].…”

Section: Rna Isolation and Rt-qpcrmentioning

confidence: 99%

See 4 more Smart Citations

Dietary Tryptophan Supplementation Alters Fat and Glucose Metabolism in a Low-Birthweight Piglet Model

et al. 2021

Self Cite

View full text Add to dashboard Cite

Low birthweight (LBW) is associated with metabolic complications, such as glucose and lipid metabolism disturbances in early life. The objective of this study was to assess: (1) the effect of dietary tryptophan (Trp) on glucose and fat metabolism in an LBW piglet model, and (2) the role peripheral 5-hydroxytryptamine type 3 (5HT3) receptors in regulating the feeding behavior in LBW piglets fed with Trp-supplemented diets. Seven-day-old piglets were assigned to 4 treatments: normal birthweight-0%Trp (NBW-T0), LBW-0%Trp (LBW-T0), LBW-0.4%Trp (LBW-T0.4), and LBW-0.8%Trp (LBW-T0.8) for 3 weeks. Compared to LBW-T0, the blood glucose was decreased in LBW-T0.8 at 60 min following the meal test, and the triglycerides were lower in LBW-T0.4 and LBW-T0.8. Relative to LBW-T0, LBW-T0.8 had a lower transcript and protein abundance of hepatic glucose transporter-2, a higher mRNA abundance of glucokinase, and a lower transcript of phosphoenolpyruvate carboxykinase. LBW-T0.4 tended to have a lower protein abundance of sodium-glucose co-transporter 1 in the jejunum. In comparison with LBW-T0, LBW-T0.4 and LBW-T0.8 had a lower transcript of hepatic acetyl-CoA carboxylase, and LBW-T0.4 had a higher transcript of 3-hydroxyacyl-CoA dehydrogenase. Blocking 5-HT3 receptors with ondansetron reduced the feed intake in all groups, with a transient effect on LBW-T0, but more persistent effect on LBW-T0.8 and NBW-T0. In conclusion, Trp supplementation reduced the hepatic lipogenesis and gluconeogenesis, but increased the glycolysis in LBW piglets. Peripheral serotonin is likely involved in the regulation of feeding behavior, particularly in LBW piglets fed diets supplemented with a higher dose of Trp.

show abstract

Section: Experimental Design and Dietsmentioning

confidence: 99%

Section: Meal Test and Blood And Tissue Collectionmentioning

confidence: 99%

Section: Diet Proximate and Amino Acids Analysismentioning

confidence: 99%

Section: Rna Isolation and Rt-qpcrmentioning

confidence: 99%

Section: Rna Isolation and Rt-qpcrmentioning

confidence: 99%

See 3 more Smart Citations

Dietary Tryptophan Supplementation Alters Fat and Glucose Metabolism in a Low-Birthweight Piglet Model

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

Multiple amino acid supplementations to reduce dietary protein for pigs during early and late finisher periods under commercial conditions

Song

et al. 2023

J Sci Food Agric

View full text Add to dashboard Cite

Background With the easy availability and competitive prices of crystalline amino acids (AAs), the reduction of dietary crude protein (CP) for pigs during early and late finisher periods is possible under commercial conditions. Two experiments were conducted to assess the growth efficiency of early and late‐finishing pigs fed with protein‐restricted diets supplemented with Lys, Met, Thr, Trp, Val, Ile and His. In Experiment 1, 840 early finishing pigs were allocated to four dietary treatments with CP levels designed at 150, 142, 134, and 126 g kg−1 diet. In Experiment 2, 768 late‐finishing pigs were allotted to four dietary treatments providing CP levels at 140, 130, 120, and 110 g kg−1 diet. Results In Experiment 1, the data showed that CP levels could be decreased from 150 to 126 g kg−1 without adversely affecting performance of early finishing pigs as no significant difference was observed for final bodyweight, average daily gain (ADG), feed to gain ratio (F:G), or average daily feed intake (ADFI). In Experiment 2, late‐finishing pigs consuming 120 g kg−1 CP tended to have the highest ADG and lowest F:G whereas those fed the 110 g kg−1 CP diet showed the opposite trend. Based on quadratic analysis, the optimum CP levels to maximize ADG and minimize F:G were 126 and 127 g kg−1, respectively. Conclusion These findings showed that dietary CP levels could be decreased to 126 g kg−1 for early finishing pigs while improved performance was noted in late‐finishing pigs consuming 120 g kg−1 CP. © 2023 Society of Chemical Industry.

show abstract

Growth performance, serum parameters, inflammatory responses, intestinal morphology and microbiota of weaned piglets fed 18% crude protein diets with different ratios of standardized ileal digestible isoleucine to lysine

Wang,

Liu,

et al. 2024

Animal Nutrition

View full text Add to dashboard Cite

Branched-chain amino acids partially recover the reduced growth of pigs fed with protein-restricted diets through both central and peripheral factors

Cited by 13 publications

References 76 publications

Dietary Tryptophan Supplementation Alters Fat and Glucose Metabolism in a Low-Birthweight Piglet Model

Dietary Tryptophan Supplementation Alters Fat and Glucose Metabolism in a Low-Birthweight Piglet Model

Multiple amino acid supplementations to reduce dietary protein for pigs during early and late finisher periods under commercial conditions

Growth performance, serum parameters, inflammatory responses, intestinal morphology and microbiota of weaned piglets fed 18% crude protein diets with different ratios of standardized ileal digestible isoleucine to lysine

Contact Info

Product

Resources

About