Chronic effects of soft drink consumption on the health state of Wistar rats: A biochemical, genetic and histopathological study

Alkhedaide, Adel; Soliman, Mohamed Mohamed; Salah-Eldin, Alaa-Eldin; Ismail, Tamer Ahmed; Alshehiri, Zafer Saad; Attia, Hossam Fouad

doi:10.3892/mmr.2016.5199

Cited by 24 publications

(26 citation statements)

References 43 publications

(48 reference statements)

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“…Alkhedaide et al, 2016 [24] mentioned that caffeine in carbonated beverages is added to make individuals addicted and is readily absorbed rapidly compared with any other drink. This might explain the increase in average Diet Coke intake in rats of group II and group IV as compared to the average water intake of control rats in the present study.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Diet Coke and Monosodium Glutamate on the Cerebellar Cortex of Adult Male Albino Rats. Histological and Immuno-histochemical Study

El-Wahed

Geith

Kalleny

et al. 2019

Egyptian Journal of Histology

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Background: The consumption of fast-food is increasing among children, adolescents and adults. Carbonated drinks as Diet Coke are widely consumed with fast food. One of the thousands of chemicals used in our new high-tech foods is the monosodium glutamate (MSG). Aim of the study: This study was conducted to investigate the effect of Diet Coke and monosodium glutamate salt either separately or in combination on the cerebellar cortex of adult male albino rats. Materials and Methods: Forty adult male albino rats were divided into four groups. Group I: control group. Group II (Diet Coke group) which was subdivided into: subgroup IIA: each rat received 2.5 ml of Diet Coke twice daily by oral gavage and subgroup IIB: rats received Diet Coke instead of water throughout the day. Group III (MSG group): rats received MSG salt solution (3 gm MSG / kgm) by oral gavage once daily. Group IV (combination group) rats received combination of MSG solution and Diet Coke. This group was subdivided into two subgroups. Subgroup IVA: rats received MSG salt solution in the same dose as group III and 2.5 ml of Diet Coke twice daily, by oral gavage. Subgroup IVB: rats received MSG salt solution in the same dose as group III and Diet Coke instead of water throughout the day. At the end of the experiment (21 days) cerebella were dissected out and processed for histological, immunohistochemical and morphometric studies. Results: Histological examination of the cerebellar cortex revealed that both Diet Coke and MSG either separately or in combination resulted in degenerative changes that were more significant in the combination group. Conclusion: Diet Coke and MSG induced degenerative changes in the cerebellar cortex that was more significant in their combination.

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Section: Discussionmentioning

confidence: 99%

The Effect of Diet Coke and Monosodium Glutamate on the Cerebellar Cortex of Adult Male Albino Rats. Histological and Immuno-histochemical Study

El-Wahed

Geith

Kalleny

et al. 2019

Egyptian Journal of Histology

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“…The inability of antioxidant defense mechanisms to scavenge excessive levels of reactive oxygen species (ROS) and/or reduction in the basal antioxidant defense mechanisms are the primary causes for oxidative stress in tissues (17). Therefore, degenerative diseases, including hepatopathies are caused by this oxidative stress (17,18). Lipid peroxidation is demonstrated to induce disturbance of membrane function and integrity (19).…”

Section: Discussionmentioning

confidence: 99%

Carbonated soft drinks alter hepatic cytochrome P450 isoform expression in Wistar rats

2016

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Abstract.The aim of the current study was to examine the effects of chronic consumption of soft drinks (SDs) on hepatic oxidative stress and cytochrome P450 enzymes (CYPs) expression in the livers of Wistar rats. For 3 consecutive months, the rats had free access to three different soft drinks, Coca-Cola, Pepsi-Cola and 7-UP. The rats were subsequently compared with control group rats that had consumed water. Blood and hepatic tissue samples were assayed for the changes in antioxidants, liver function biomarkers and hepatic gene expression for different isoforms of hepatic CYP. The results indicated that SD consumption (SDC) decreased serum antioxidant levels and increased malondialdehyde secretion, and increased liver biomarkers (glutamate pyruvate transaminase and glutamate oxaloacetate). SD induced alterations in mRNA expression of hepatic antioxidants and cytochrome isoforms. The expression of peroxidase, catalase, CYP1A2, CYP3A2 and CYP2C11 in the liver were upregulated following SDC. By contrast, CYP2B1 was downregulated after 3 months of SDC in liver tissue samples. Thus, the present findings indicate that SDs induced oxidative stress in the liver of Wistar rats and for the first time, to the best of our knowledge, indicate that SDC disrupts hepatic CYP enzymes that may affect drug metabolism. Therefore, drug-dosing programs should be carefully designed to take these novel findings into consideration for the treatment of diseases.

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“…The results demonstrated that carbonated beverages induced oxidative stress and change of the expression of certain genes associated with brain activity. Alkhedaide in his study also associated the consumption of soft drinks in rodents with the increase of oxidative stress biomarkers, and besides that with a disruption in the expression of certain genes associated with liver and kidney functions [8] [9].…”

Section: Introductionmentioning

confidence: 96%

Effects of Gaseous Drinks in Wistar Rats Esophagus

Oliveira¹,

Gaspar²,

Braga³

et al. 2017

JBM

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The consumption of beverages and processed foods, mainly soft drinks, has been incorporated into the usual diet of children, adolescents and adults frequently in recent years. The present study aimed to study the effects of carbonated drinks on Wistar rats, based on the macroscopic and histological morphology of the esophagus. Fifteen Wistar rats were divided into three groups: control group; group fed with ration and cola-type refrigerant ad libitum and group fed with ration and gas industrialized water ad libitum, for a trial period of 90 days. For histopathology and morphology, the esophagus was removed, prepared in slides and stained with hematoxylin-eosin. Images of the sections were captured for analysis and classification of the inflammatory infiltrate. For morphometry, ten grid fields of 100 points were evaluated in the 40x eyepiece, totaling 3000 points per animal. The results demonstrate that the refrigerant treatment induced the following structural changes in the rats: significant reduction of weight in relation to the control group; inflammatory infiltrate predominantly diffuse mild to moderate and tissue edema. The rats treated with carbonated water had similar results to the control, besides signs of healing and tissue repair.

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Chronic effects of soft drink consumption on the health state of Wistar rats: A biochemical, genetic and histopathological study

Cited by 24 publications

References 43 publications

The Effect of Diet Coke and Monosodium Glutamate on the Cerebellar Cortex of Adult Male Albino Rats. Histological and Immuno-histochemical Study

The Effect of Diet Coke and Monosodium Glutamate on the Cerebellar Cortex of Adult Male Albino Rats. Histological and Immuno-histochemical Study

Carbonated soft drinks alter hepatic cytochrome P450 isoform expression in Wistar rats

Effects of Gaseous Drinks in Wistar Rats Esophagus

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