Chromosome damage in early embryo and endosperm development in crosses involving the preferentially transmitted 4S1 chromosome of Aegilops sharonensis

King, Ian P.; Laurie, D. A.

doi:10.1038/hdy.1993.7

Cited by 17 publications

(20 citation statements)

References 13 publications

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“…This mosaicism implies that chromosome 2C can also induce aberrations in zygotes. King and Laurie (1993) reported that similar post-fertilization aberrations were induced by another gametocidal chromosome 4S l from Aegilops sharonensis. Although the plants carrying 7H aberrations were expected to have one normal 7H chromosome (Fig.…”

Section: Resultsmentioning

confidence: 92%

Genetic induction of chromosomal rearrangements in barley chromosome 7H added to common wheat

Shi

Endo

2000

Chromosoma

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Chromosome 2C of Aegilops cylindrica induces chromosomal rearrangements in alien chromosome addition lines, as well as in euploid lines, of common wheat. To induce chromosomal rearrangements in barley chromosome 7H, reciprocal crosses were made between a mutation-inducing common wheat line that carries a pair of 7H chromosomes and one 2C chromosome and a 7H disomic addition line of common wheat. Many shrivelled seeds were included in the progeny, which was an indication of the occurrence of chromosome mutations. The chromosomal constitution of the viable progeny was examined by FISH (fluorescence in situ hybridization) using the barley subterminal repeat HvT01 as a probe. Structural changes of chromosome 7H were found in about 15% of the progeny of the reciprocal crosses. The aberrant 7H chromosomes were characterized by a combination of N-banding, FISH and genomic in situ hybridization. Mosaicism for aberrant 7H chromosomes was observed in seven plants. In total, 89 aberrant 7H chromosomes were identified in 82 plants, seven of which had double aberrations. More than half of the plants carried a simple deletion: four short-arm telosomes, one long-arm telosome, and 45 terminal deletions (23 in the short arm, 21 in the long arm, and one involving both arms). About 40% of the aberrations represented translocations between 7H and wheat chromosomes. Twenty of the translocations had wheat centromeres, 12 the 7H centromere, with translocation points in the 7HS (five) and in the 7HL (seven), and the remaining four were of Robertsonian type, three involving 7HS and one with 7HL. In addition, one translocation had a barley segment in an intercalary position of a wheat chromosome, and two were dicentric. The breakpoints of these aberrations were distributed along the entire length of chromosome 7H.

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Section: Resultsmentioning

confidence: 92%

Genetic induction of chromosomal rearrangements in barley chromosome 7H added to common wheat

Shi

Endo

2000

Chromosoma

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“…The speltoid morphology in these plants may be the result of spontaneous deletions or deletions brought about by the action of the gene responsible for chromosome fragmentation located on chromosome 4S l (King & Laurie, 1993), which have resulted in the loss of the Q gene.…”

Section: Resultsmentioning

confidence: 99%

“…The reduction in 1000 grain weight and ear yield could be due to chromosome 4S l induced endosperm damage (King & Laurie, 1993) or deleterious genes located on the 4S l chromosome segment.…”

Section: Discussionmentioning

confidence: 99%

An assessment of the potential of 4DS.4DL-4s l L translocation lines as a means of eliminating tall off types in semi-dwarf wheat varieties

et al. 1996

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“…Previous studies have indicated that Gc genes ensure their preferential transmission by causing chromosomal breaks in gametophytes lacking Gc genes (Finch et al 1984;King and Laurie 1993;Nasuda et al 1998). The action of the Gc2 gene located on the Ae.…”

Section: Introductionmentioning

confidence: 97%

Characterization of a knock-out mutation at the Gc2 locus in wheat

et al. 2003

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Gametocidal (Gc) genes, introduced into common wheat from related Aegilops species, are selfish genetic elements that ensure their preferential transmission by inducing chromosomal breaks. Here we report the production and characterization of a knock-out mutation of the Gc2 gene transferred to wheat as a wheat-Aegilops sharonensis T4B-4S(sh)#1 translocation chromosome. In hemizygous Gc2/- condition, gametophytes lacking Gc2 suffer chromosomal fragmentation and produce non-functional gametes, which leads to sporophytic semisterility and exclusive transmission of the Gc2-carrier chromosome. We have identified one putative ethyl methylsulfonate (EMS)-induced Gc2 mutant that restores spike fertility and shows Mendelian segregation. Progeny screening mapped the mutation to the Gc2-carrier chromosome T4B-4S(sh)#1. C-banding and fluorescence in situ hybridization analyses showed that the loss of Gc2 function in the mutant is not due to a terminal deficiency. Analysis of first and second pollen mitoses in Gc2(mut) /- plants and C-banding analysis of testcross progenies showed that no chromosomal breakage occurs in the mutant. No gametophytic chromosomal breakage was observed in heterozygous Gc2(mut) /Gc2 plants, which had fully fertile spikes. These results suggest that Gc2 encodes two agents, one causing chromosomal breaks in gametophytes lacking Gc2 and another that protects the Gc2 carrier from breakage. The EMS-induced Gc2 mutant appears to be a knock-out of the gene encoding the "breaking" agent. These data are a first crucial step toward the molecular understanding of Gc2 action.

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Chromosome damage in early embryo and endosperm development in crosses involving the preferentially transmitted 4S1 chromosome of Aegilops sharonensis

Cited by 17 publications

References 13 publications

Genetic induction of chromosomal rearrangements in barley chromosome 7H added to common wheat

Genetic induction of chromosomal rearrangements in barley chromosome 7H added to common wheat

An assessment of the potential of 4DS.4DL-4s l L translocation lines as a means of eliminating tall off types in semi-dwarf wheat varieties

Characterization of a knock-out mutation at the Gc2 locus in wheat

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