Chromosomal reallocation of the chicken <i>c‐myb</i> locus and organization of 3′‐proximal coding exons

Soret, Johann; Vellard, Michel; Viegas‐Péquignot, Evani; Apiou, Françoise; Dutrillaux, Bernard; Perbal, Bernard

doi:10.1016/0014-5793(90)81387-4

Cited by 7 publications

(2 citation statements)

References 47 publications

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“…Shaw et al (1991) and Tereba et al (1991) have located the genes for growth hormone and aromatase, respectively, to the q arm of Chromosome 1. The newer fluorescent probe methods are now being used in chickens for the location of cloned genes by several groups (Soret et al, 1990;Dominguez-Steglisch et al, 1990, 1991, 1992aHutchison and LeCeil, 1992;Li and Ponce de Leon, 1992; N. J. Hutchison and C. LeCeil, Fred Hutchinson Cancer Research Center, 1124 Columbia St., Seattle, WA 98104, personal communication; F. A. Ponce de Leon, Department of Veterinary and Animal Science, University of Massachusetts, Amherst, MA 01003, personal communication).…”

Section: Introductionmentioning

confidence: 99%

Characterization of a Red Jungle Fowl by White Leghorn Backcross Reference Population for Molecular Mapping of the Chicken Genome

et al. 1993

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A reference population designed for molecular genetic mapping of the chicken genome was produced by backcrossing a partially inbred Red Jungle Fowl (JF) line to a highly inbred White Leghorn (WL) line. The parental lines were chosen to maximize the expected genetic polymorphisms between them. Two full-sib F^ males, produced by crossing a JF male with a WL female, were each individually mated to about 10 WL females to produce 400 progeny. All the progeny were classified for segregation of three loci controlling color phenotype and six blood group loci, some of which have been mapped by classical methods. Segregation of these nine loci did not differ significantly from the expected 1:1 ratio with one exception. At least 20 mL of whole blood was stored from all the parents and progeny to provide DNA for molecular analysis. Screening of the parental lines and Fi crosses by Southern blot with cloned genes and by the random amplified polymorphic DNA (RAPD) procedure revealed a large number of molecular markers mat were parental line-specific. A preliminary analysis of 16 backcross progeny classified for polymorphisms at 2 color loci, 6 blood group loci, 16 loci detected by cloned chicken genes, and 4 loci detected by the RAPD method has been completed. Segregation at 27 out of 28 loci did not differ significantly from the expected 1:1 ratio, showing that two alternative alleles were detected at each locus. Five pairs of linked loci were detected (P < .01). Thus, this population is polymorphic and gives simple segregation for two types of molecular probes, providing a good resource for collaborative mapping of the chicken genome. (

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Section: Introductionmentioning

confidence: 99%

Characterization of a Red Jungle Fowl by White Leghorn Backcross Reference Population for Molecular Mapping of the Chicken Genome

et al. 1993

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“…In most of these cases, the oncogenic activation of c-myb results in the expression of truncated MYB proteins deprived of the casein kinase II phosphorylation site and of the 5Ј-proximal (R1) DNA-binding repeat which has been proposed to play a role in the restricted transforming potential of c-myb (38,43,62). According to the previously established nomenclature (72), the RAV-1 insertion occurred upstream from c-myb exons 3 (33) and 2 (60) in B-cell lymphomas. The RAV-1 insertional activation of c-myb in T9 cells results in the expression of a 66-kDa C-MYB protein which is deprived of sequences encoded by exons 1 to 3.…”

Section: Discussionmentioning

confidence: 99%

Retroviral insertional activation of the c-myb proto-oncogene in a Marek's disease T-lymphoma cell line

Rouzic

Perbal

1996

J Virol

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Marek's disease virus (MDV) is an avian herpesvirus that causes, in chickens, a lymphoproliferative disease characterized by malignant transformation of T lymphocytes. The rapid onset of polyclonal tumors indicates the existence of MDV-encoded oncogenic products. However, the molecular basis of MDV-induced lymphoproliferative disease and latency remains largely unclear. Several lines of evidence suggest that MDV and Rous-associated virus (RAV) might cooperate in the development of B-cell lymphomas induced by RAV. Our present results indicate for the first time that MDV and RAV might also act synergistically in the development of T-cell lymphomas. We report an example of an MDV-transformed T-lymphoblastoid cell line (T9) expressing high levels of a truncated C-MYB protein as a result of RAV integration within one c-myb allele. The chimeric RAV-c-myb mRNA species initiated in the 5 long terminal repeat of RAV are deprived of sequences corresponding to c-myb exons 1 to 3. The attenuation of MDV oncogenicity has been strongly related to structural changes in the MDV BamHI-D and BamHI-H DNA fragments. We have established that both DNA restriction fragments are rearranged in the T9 MDV-transformed cells. Our results suggest that retroviral insertional activation of the c-myb proto-oncogene is a critical factor involved in the maintenance of the transformed phenotype and the tumorigenic potential of this T-lymphoma cell line.

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Chicken genome mapping: a new era in avian genetics

Bumstead²,

et al. 1995

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Chromosomal reallocation of the chicken c‐myb locus and organization of 3′‐proximal coding exons

Cited by 7 publications

References 47 publications

Characterization of a Red Jungle Fowl by White Leghorn Backcross Reference Population for Molecular Mapping of the Chicken Genome

Characterization of a Red Jungle Fowl by White Leghorn Backcross Reference Population for Molecular Mapping of the Chicken Genome

Retroviral insertional activation of the c-myb proto-oncogene in a Marek's disease T-lymphoma cell line

Chicken genome mapping: a new era in avian genetics

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