Chromosomal assignments for porcine genes encoding enzymes in hepatic metabolic pathways

Abstract

Increasing the number of mapped genes will facilitate (1) the identification of potential candidate genes for a trait of interest within quantitative trait loci regions and (2) comparative mapping. The metabolic activities of the liver are essential for providing fuel to peripheral organs, for regulation of amino acid, carbohydrate and lipid metabolism and for homoeostasis of vitamins, minerals and electrolytes. We aimed to identify and map genes coding for enzymes active in the liver by somatic cell genetics in order to contribute to the improvement of the porcine gene map. We mapped 28 genes of hepatic metabolic pathways including six genes whose locations could be confirmed and 22 new assignments. Localization information in human was available for all but one gene. In total 24 genes were assigned to in the expected chromosomal regions on the basis of the currently available information on the comparative human and pig map while for four genes our results suggest a new correspondence or extended regions of conservation between porcine and human chromosomes.

Improving the comparative map of porcine chromosome 10 with respect to human chromosomes 1, 9 and 10

“…The previously mapped porcine EST SSC25A02 (Hawken et al, 1999) is homologous to NUDT2, so there was no need to further map this gene on the IMpRH. GALT was linked to SSC10G07 on SSC10 using the IMpRH, contradicting a published position (Pinton et al, 2000) on SSC14 based on heterologous FISH using a goat bacterial artificial chromosome (BAC) on porcine chromosomes but agreeing with a recent report (Wimmers et al, 2002) mapping it adjacent to SSC10G07. The gene order GALT-HSPC177-ACO1-DNAJA1-DCTN3-OPRS1 is rearranged with respect to the human.…”