Chromosomal abnormalities in embryos derived from testicular sperm extraction

Single blastocyst transfer after ICSI from ejaculate spermatozoa, percutaneous epididymal sperm aspiration (PESA) or testicular sperm extraction (TESE)

“…Aneuploid [24] or mosaic [25] spermatozoa in testicular spermatozoa is found in a higher frequency in men with azoospermia, in particular non-obstructive [26] than in normal sperm. Also, chromosome Y deletions will be inherited by male offspring [36].…”

Male factor infertility and ART

“…88,89 When epididymal sperm is used for ICSI, stillbirths or congenital malformations are not more prevalent in comparison to IVF and ICSI using ejaculated sperm, while cognitive development was also similar. 90,91 While aneuploidy screening on embryos obtained after ICSI for NOA showed increased aneuploidy and mosaicism, 92 and karyotypes of miscarriages occurring after TESE-ICSI showed higher aneuploidy rates than expected, 93 the few publications focusing on the outcome of children born after ICSI using testicular sperm conclude that no significant differences exist between ICSI using ejaculated spermatozoa or ICSI using testicular spermatozoa in terms of birth weight, perinatal mortality and major malformation rate. 90,91,[94][95][96] Subgroup analysis on children born from men with NOA showed no increase in malformation and early perinatal mortality rates in comparison to the rates observed in ICSI children after using ejaculated sperm.…”

Human Male Meiosis and Sperm Aneuploidies

“…A similar study in couples with abnormal FISH and low sperm concentration (<5X10 6 sperm/mL) also found a significant increase in abnormal embryos, especially in mosaic embryos -embryos with cells with different chromosome complement-and sex chromosome aneuploidies (Pehlivan et al, 2003). Mosaicism rate as high as 53% has been reported in patients with NOA (Silber et al, 2003). These findings could be explained by fertilization with sperm carrying multiple chromosomal alterations or centrosome abnormalities.…”