Chromium (VI) tolerance and bioaccumulation by Candida tropicalis isolated from textile wastewater

Ilyas, Sidra; Bukhari, Dilara Abbas; Rehman, Abdul

doi:10.1186/s42834-020-00069-1

Cited by 11 publications

(3 citation statements)

References 44 publications

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“…The accumulation capacity of Cr 6+ by strain R‐MT1/YieF was considerably decreased by the presence of Ag +1 , Hg 2+ and Cd 2+ in the culture medium. This may due to the inhibitory effect of these metal ions on the activity of chromate reductase 29,30 …”

Section: Discussionmentioning

confidence: 99%

Enhancement of chromate bioaccumulation by engineered Escherichia coli cells co‐expressing chromate reductase (YieF) and a rice metallothionein isoform (OsMT1)

Shahpiri

Majnoun

Kazemi‐Nasab

et al. 2021

J of Chemical Tech & Biotech

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BACKGROUND Hexavalent chromium Cr(VI) is a toxic and carcinogenic heavy metal. It is highly water soluble and one of the most widely used metals in industry, resulting in soil and water contamination. Nowadays engineering microbes provide new strategies for remediation of heavy metals. RESULTS In the present work, a gene encoding E. coli chromate reductase (YieF) was cloned in pCDF‐1b and was transferred to both cells containing empty pET41a (control strain), and cells containing pET41a‐OsMT1 (R‐MT1) resulted in production of new strains R‐YieF and R‐YieF/MT1, respectively. Both proteins – YieF as His‐tag fusion protein and OsMT1 as glutathione‐S‐transferase fusion protein – appeared in the soluble fraction of R‐YieF/MT1 after induction with isopropyl‐d‐1‐thiogalactopyranoside . The strain R‐YieF/OsMT1 showed high tolerance to Cr6+ and accumulated significantly higher Cr6+ than R‐MT1 (5 mg L−1), R‐YieF (7 mg L−1) and control (2 mg L−1). The accumulation of Cr6+ was dependent on the initial concentration of Cr6+ in the medium, time and the presence of other toxic metals such as Ag+1, Hg+2 and Cd2+. CONCLUSION This study demonstrated that the co‐expression of YieF and OsMT1 could effectively enhance the accumulation of Cr(VI) by E. coli cells and opens new insights into bioremediation strategies. © 2020 Society of Chemical Industry

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Section: Discussionmentioning

confidence: 99%

Enhancement of chromate bioaccumulation by engineered Escherichia coli cells co‐expressing chromate reductase (YieF) and a rice metallothionein isoform (OsMT1)

Shahpiri

Majnoun

Kazemi‐Nasab

et al. 2021

J of Chemical Tech & Biotech

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“…Resistance in yeast is mostly due to the limited Cr uptake, by direct transformation of Cr(VI) to Cr(III) or by secreting reducing agents that reduce Cr(VI). A yeast isolated from industrial wastewater (Candida tropicalis), showed the ability to uptake Cr(VI) and reduce it to Cr(III; Ilyas et al, 2020). The yeasts S. cerevisiae and Pichia guilliermondii were able to reduce Cr(VI) and produce a chelating agent that trapped Cr(III) extracellularly when grown in the presence of Cr(VI) (Ksheminska et al, 2006).…”

Section: Direct Resistance Mechanismsmentioning

confidence: 99%

The Microbiology of Metal Mine Waste: Bioremediation Applications and Implications for Planetary Health

Newsome

Falagán

2021

GeoHealth

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Microbes colonise and inhabit mine wastes, they tolerate high concentrations of metals and contribute to soil functioning and plant growth  Microbes transform metal speciation and environmental mobility, through metabolism, biogeochemical cycling and metal resistance mechanisms  Beneficial microbial activity can be stimulated to remediate metal-containing mine wastes, but more long-term field studies are required

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“…The toxic effect of heavy metals on microorganisms is mediated, for example, by inhibition of enzyme activity or by alterations in protein synthesis and ATP production ( 21 ). There are practically non-existent data that show the levels of Cr (III) or Cr (VI) after the chemical or enzymatic hydrolysis of the wastes ( 22 , 23 ).…”

Section: Introductionmentioning

confidence: 99%

Biotransformation of protein-rich waste by Yarrowia lipolytica IPS21 to high-value products—amino acid supernatants

Wieczorek,

Gendaszewska,

Miśkiewicz

et al. 2023

Microbiol Spectr

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The yeast strain Yarrowia lipolytica IPS 21 was tested for its ability to degrade potentially toxic chrome-tanned leather shavings (CTLS) in a liquid environment. Biological and chemical parameters were monitored during a 48-h period of biotransformation of the protein-rich waste. CTLS was added at a concentration of 0.1–4% (wt/wt) to a modified YPG medium (15 g L −1 yeast extract and 5 g L −1 NaCl). Biodegradation and bioconversion were performed in a one-step process. It was found that the higher degradation rate depended on the activity of the proteases and the pH of the medium, but not on the initial inoculum ratio and the activity of the dehydrogenase. The highest efficiency of the process was obtained for 4% (wt/wt) CTLS on day 2 (degradation rate 58–67%, biomass production 2.11–2.20 g L −1 , protease activity 312 U mg −1 protein, and pH 9.20). Our results showed that total chromium was probably not transported across the cytoplasmic membrane of Y. lipolytica IPS21 and that chromium (III) was not oxidized to chromium (VI). The phytotoxicity of selected amino acid supernatants [2.5% (vol/vol)] was tested after the bioconversion process. It was found that the supernatants had a stimulating effect on the plants tested. The root elongation was 29–28% higher than that of the reference samples. This result makes Y. lipolytica IPS21 a potential candidate for safely converting potentially toxic protein-rich wastes into valuable products without enzyme isolation, e.g., amino acid fertilizers. IMPORTANCE Enzyme technologies have the greatest practical relevance to environmental trends. Overcoming the barrier of the high cost of carbon substrates used for biotransformation is the main challenge of these methods. The huge potential of the use of extracellular proteases of Yarrowia species or amino acids in various industries indicates the need for the extension of basic research on waste as a carbon source for this yeast. The experiments demonstrated that it is possible to use Y. lipolytica IPS21 for bioconversion of chrome-tanned leather shavings (CTLS) in a single-step process and to produce high-value amino acid supernatant without having an isolated enzyme. In our study, we show the effect of 2.5% (vol/vol) CTLS supernatant obtained from Y. lipolytica IPS21 on the elongation of the root system of selected plants and provide information on the effect of environmental factors on the efficiency of the bioconversion and the migration of chromium.

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Chromium (VI) tolerance and bioaccumulation by Candida tropicalis isolated from textile wastewater

Cited by 11 publications

References 44 publications

Enhancement of chromate bioaccumulation by engineered Escherichia coli cells co‐expressing chromate reductase (YieF) and a rice metallothionein isoform (OsMT1)

Enhancement of chromate bioaccumulation by engineered Escherichia coli cells co‐expressing chromate reductase (YieF) and a rice metallothionein isoform (OsMT1)

The Microbiology of Metal Mine Waste: Bioremediation Applications and Implications for Planetary Health

Biotransformation of protein-rich waste by Yarrowia lipolytica IPS21 to high-value products—amino acid supernatants

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