The Microbiology of Metal Mine Waste: Bioremediation Applications and Implications for Planetary Health

Newsome, Laura; Falagán, Carmen

doi:10.1029/2020gh000380

Cited by 35 publications

(18 citation statements)

References 474 publications

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“…P. pseudoalcaligenes CECT 5344 was grown with different concentrations of arsenite (up to 1 mM), using either ammonium or cyanide as the sole nitrogen source ( Figure 1 B). It was observed that, with both nitrogen sources, a concentration of 0.25 mM of arsenite, almost 10 times higher than the average concentration of arsenic in the lithosphere [ 51 ], conditioned cell growth sufficiently without being highly detrimental to bacterial growth ( Figure 1 B). Likewise, P. pseudoalcaligenes CECT 5344 was highly resistant to As(V), but it was observed that 200 mM arsenate negatively affected bacterial growth ( Figure S1 ).…”

Section: Resultsmentioning

confidence: 99%

Proteomic Analysis of Arsenic Resistance during Cyanide Assimilation by Pseudomonas pseudoalcaligenes CECT 5344

Biełło

Cabello

Rodríguez-Caballero

et al. 2023

IJMS

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Wastewater from mining and other industries usually contains arsenic and cyanide, two highly toxic pollutants, thereby creating the need to develop bioremediation strategies. Here, molecular mechanisms triggered by the simultaneous presence of cyanide and arsenite were analyzed by quantitative proteomics, complemented with qRT-PCR analysis and determination of analytes in the cyanide-assimilating bacterium Pseudomonas pseudoalcaligenes CECT 5344. Several proteins encoded by two ars gene clusters and other Ars-related proteins were up-regulated by arsenite, even during cyanide assimilation. Although some proteins encoded by the cio gene cluster responsible for cyanide-insensitive respiration decreased in the presence of arsenite, the nitrilase NitC required for cyanide assimilation was unaffected, thus allowing bacterial growth with cyanide and arsenic. Two complementary As-resistance mechanisms were developed in this bacterium, the extrusion of As(III) and its extracellular sequestration in biofilm, whose synthesis increased in the presence of arsenite, and the formation of organoarsenicals such as arseno-phosphoglycerate and methyl-As. Tetrahydrofolate metabolism was also stimulated by arsenite. In addition, the ArsH2 protein increased in the presence of arsenite or cyanide, suggesting its role in the protection from oxidative stress caused by both toxics. These results could be useful for the development of bioremediation strategies for industrial wastes co-contaminated with cyanide and arsenic.

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Section: Resultsmentioning

confidence: 99%

Proteomic Analysis of Arsenic Resistance during Cyanide Assimilation by Pseudomonas pseudoalcaligenes CECT 5344

Biełło

Cabello

Rodríguez-Caballero

et al. 2023

IJMS

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“…Thus, once oxidised, potentially toxic elements previously adsorbed onto the surface or associated with pyrite are released to the solution [7,8]. Although many acidophiles can obtain carbon autotrophically, their growth and metabolic activity in mine waste may be limited by the availability of other essential nutrients, such as nitrogen and phosphorus [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Evolution of Sulfidic Legacy Mine Tailings: A Review of the Wheal Maid Site, UK

Fitch

Parbhakar-Fox²,

Crane

et al. 2022

Minerals

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Historic tailings dams and their associated mine waste can pose a significant risk to human and environmental health. The Wheal Maid mine site, Cornwall, UK, serves as an example of the temporal evolution of a tailings storage facility after mining has ceased and the acid-generating waste subjected to surficial processes. This paper discusses its designation as a contaminated land site and reviews our current understanding of the geochemistry, mineralogy, and microbiology of the Wheal Maid tailings, from both peer-reviewed journal articles and unpublished literature. We also present new data on waste characterisation and detailed mineral chemistry and data from laboratory oxidation experiments. Particularly of interest at Wheal Maid is the presence of pyrite-bearing “Grey Tailings”, which, under typical environmental conditions at the Earth’s surface, would be expected to have undergone oxidation and subsequently formed acidic and metalliferous mine drainage (AMD). The results identified a number of mechanisms that could explain the lack of pyrite oxidation in the Grey Tailings, including a lack of nutrients inhibiting microbial Fe(II) oxidation, passivation of pyrite mineral surfaces with tailings processing chemicals, and an abundance of euhedral pyrite grains. Such research areas need further scrutiny in order to inform the design of future tailings facilities and associated AMD management protocols.

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“…Colony growth of SRB can be inhibited by extreme conditions such as high pressure, radiation, and heat. However, because SRB is a spore-forming bacteria that can endure harsh conditions, the growth activity cannot be eliminated [32,33].…”

Section: Discussionmentioning

confidence: 99%

Sulphate-reducing bacteria (SRB) in interim storage of spent nuclear fuel

Rahayu,

Ambarsari,

Shalsabilla

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Interim Storage of Spent Nuclear Fuel (ISSF) is an installation for temporarily storing spent nuclear fuels. Nuclear fuel storage pools must be free from contamination by corrosion-causing microorganisms such as Sulphate Reduction Bacteria (SRB). This research aims to detect SRB on the pool’s wall, floor, and spent nuclear fuel racks. The measured parameters consisted of physicochemical, total bacteria, total SRB, and detection of SRB on the wall, floor, and spent fuel rack in the pool using the SRB kit. The results showed that the quality of the water chemistry in the pool was within limits according to the provisions of the International Atomic Energy Agency, except for the total bacteria (> 1000 CFU/ml). In the water pools around the racks of spent fuels, SRB was also detected at 30 CFU/ml, while the level of SRB on the walls, floors, and spent nuclear fuel racks showed varied values from moderate to severe. This research is important especially to identify corrosion-causing microbes for water and biofilm samples. This is the first step in understanding the problem of biofilm formation and contamination of microorganisms in spent fuel storage pools.

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The Microbiology of Metal Mine Waste: Bioremediation Applications and Implications for Planetary Health

Cited by 35 publications

References 474 publications

Proteomic Analysis of Arsenic Resistance during Cyanide Assimilation by Pseudomonas pseudoalcaligenes CECT 5344

Proteomic Analysis of Arsenic Resistance during Cyanide Assimilation by Pseudomonas pseudoalcaligenes CECT 5344

Evolution of Sulfidic Legacy Mine Tailings: A Review of the Wheal Maid Site, UK

Sulphate-reducing bacteria (SRB) in interim storage of spent nuclear fuel

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