Chromatography separation, 1H NMR analysis and bioautography screening of methanol extract of Excoecaria agallocha L. from Bhitarkanika, Orissa, India

Patra, Jayanta Kumar; Gouda, Sushanto; Sahoo, Sudhir Kumar; Thatoi, Hrudayanath

doi:10.1016/s2221-1691(12)60129-4

Cited by 47 publications

(42 citation statements)

References 10 publications

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“…Hence, the result obtained in the present study shows that the methanolic extract of E. agallocha has potential antioxidant properties as in agreement with previous study by 14,39,41,42 . Therefore, E. agallocha can be used as alternative to the antibiotics for the treatment or prevention of bacterial infections in fish.…”

Section: Discussionsupporting

confidence: 93%

“…All pure solvents separated into seven bands and showed the presence of phenolic compounds. The results of the present study are corroborated with previous findings of 14,38 that TLC chromatograms of plant extracts showed the presence of phenolic compounds, such as tannins and flavonoids and these compounds possess diverse biological properties such as anti-inflammatory, anti-bacterial and antioxidant activities. Supporting the results of the present study, 39 showed that E. agallocha bark fractions were a potential source of antioxidants and various phytochemicals give different Rf values in different solvent system.…”

Section: Discussionsupporting

confidence: 92%

“…In comparison with the result of the study of 14 31,32 . The results of the present study are corroborated by the findings of 33 that the highest antibacterial activities in mangrove halophytes were due to the presence of high content of phenols, which include phytochemicals such as; alkaloids, tannins and flavonoids, are present in the E. agallocha extract.…”

Section: Discussionmentioning

confidence: 85%

“…The anti-oxidative activity of E. agallocha leaf extract was investigated and the results suggested that environmental plants exposed to strong sunlight might require an efficient antioxidant system 12 . Previously, ethanol extract of E. agallocha showed significant antioxidant activity 13,14 .While methanolic crude extract of E. agallocha leaves had antioxidant and antibacterial activity 8,15,16 . Thus, considering the biomedical importance of mangroves, the present study was carried out to determine the phytochemical, antimicrobial and antioxidant properties of E.agallocha leaf extract.…”

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confidence: 98%

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Phytochemical Composition and In vitro Antimicrobial, Antioxidant Activites of Methanolic Leaf Extracts From Excoecaria agallocha

A.G

et al. 2016

Biosci., Biotech. Res. Asia

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This study investigated the phytochemical, antimicrobial and antioxidant properties of methanolic leaf fractionated extracts of Excoecaria agallocha. Antibacterial activity test of fractionated extract of Excoecaria agallocha was conducted via Disk diffusion assay. Micro titer broth dilution technique was performed to determine the minimum inhibitory concentration (MIC) and Minimum Bactericidal Concentration (MBC). Qualitative and quantitative DPPH radical scavenging activity determines the antioxidant activity in E. agallocha. Chromatographic separation was completed using thin layer chromatography (TLC) and column chromatographic techniques. Phytochemical screening of fractionated extract revealed active constituents including alkaloids, saponins, flavonoids, terpenoid and tannins. In vitro antibacterial activity of columnfractionated extract demonstrated inhibitory effect against Flavobacterium indicum, Chryseobacterium meningosepticum, Chryseobacterium indologenes and Chryseobacterium gleum isolated from diseased fish. MIC and MBC of E. agallocha fractionated extract was 1.56 mg/ml and 3.12 mg/ml, respectively. TLC-based qualitative DPPH analysis revealed antioxidant activity in E. agallocha extract, specified by yellowish stain on maroon color TLC plate. These bands indicate the presence of various compounds that could act as potential antimicrobial agents. The present study indicates that E. agallocha leaves contain active phytochemicals which inhibit bacteria and could be useful in the treatment of microbial infections in fish.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 85%

mentioning

confidence: 98%

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Phytochemical Composition and In vitro Antimicrobial, Antioxidant Activites of Methanolic Leaf Extracts From Excoecaria agallocha

A.G

et al. 2016

Biosci., Biotech. Res. Asia

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“…This confirmed that the presence of antimicrobial compound in the ethyl acetate extract, which was active against MRSA strain. Detection of antimicrobial compound by TLC-bioautography is one of the economical, simplest and reproducible methods for drug discovery from natural products (Hota 2010; Patra et al 2012). The antimicrobial activity of ethyl acetate extract of Phomopsis sp.…”

Section: Resultsmentioning

confidence: 99%

Molecular profiling and antimicrobial potential of endophyticGliomastix polychromaCLB32 inhabitingCombretum latifoliumBlume

et al. 2015

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Fungal endophytes as a source of bioactive metabolites have led to the development of pharmaceutical products finding new applications. In a survey of endophytic fungal biodiversity, an antimicrobial endophytic strain CLB32 was isolated from the leaf of Combretum latifolium Blume (Combretaceae) from the Western Ghats of Southern India. CLB32 was then identified as Gliomastix polychroma (KR704576) by morphological and phylogenetic analysis based on internal transcribed spacer (ITS) nuclear rDNA and intervening 5.8S rRNA gene. CLB32 here constituted the first report on incidence of endophytic fungi from C. latifolium Blume. Ethyl acetate fraction of strain CLB32 was evaluated for antimicrobial activity by disc diffusion assay. Secondary metabolites produced effectively inhibited methicillin-resistant Staphylococcus aureus (18.33 ± 0.33 mm), Pseudomonas aeruginosa (14.66 ± 0.33 mm) and Candida albicans (14.00 ± 0.57 mm). Biosynthesis of these antimicrobial compounds was detected by analytical TLC-bioautography method as depicted by zone of inhibition on intensive the band. These findings suggest that G. polychroma CLB32, as a producer of natural antimicrobial drugs, could help to combat against multidrug-resistant infections and also provide baseline information for industrial applications.

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In vitro inhibition potential of mono‐n‐octyl phthalate on Mycobacterium tuberculosis H37Ra: Possibility of binding to mycobacterial PknB—An in silico approach

Muniyan

Sivaramakrishna

Sabareesh

et al. 2018

Biotech and App Biochem

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Fatty acids of specific chain lengths have been shown to inhibit the growth of Mycobacterium tuberculosis. In the present study, specific synthetic aromatic derivatives of n-octyl esters were investigated for their property to inhibit the growth of M. tuberculosis H37Ra. Agar well diffusion assay indicated that the crude synthetic derivatives obtained by the esterification of phthalic acid (PA) and n-octanol exhibited antimycobacterial activity. Further, the activity was authenticated with the Miroplate Alamar Blue Assay (MABA). Subsequently, the active component was purified by bioactivity guided chromatographic fractionation. The structure of the synthetic derivative was deduced by UV-Vis, FT-IR, LC-MS, GC-mass spectrometry, and NMR spectroscopy. Molecular docking and molecular dynamic simulation (MDS) were performed with Autodock 4.0 and GROMACS 5.1.2 softwares, respectively. It was found that mono-n-octyl phthalate (MOP) exhibited antimycobacterial activity with a MIC of 20 μg/mL, and not by any other related compounds, including di-n-octyl phthalate, PA, phthalic anhydride, and n-octanol. Binding of MOP with protein kinase B can participate in the binding cavity region, which was previously reported. Subsequently, we authenticate the stability with MDS. This is first report on the inhibition of M. tuberculosis growth by MOP.

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Chromatography separation, 1H NMR analysis and bioautography screening of methanol extract of Excoecaria agallocha L. from Bhitarkanika, Orissa, India

Cited by 47 publications

References 10 publications

Phytochemical Composition and In vitro Antimicrobial, Antioxidant Activites of Methanolic Leaf Extracts From Excoecaria agallocha

Phytochemical Composition and In vitro Antimicrobial, Antioxidant Activites of Methanolic Leaf Extracts From Excoecaria agallocha

Molecular profiling and antimicrobial potential of endophyticGliomastix polychromaCLB32 inhabitingCombretum latifoliumBlume

In vitro inhibition potential of mono‐n‐octyl phthalate on Mycobacterium tuberculosis H37Ra: Possibility of binding to mycobacterial PknB—An in silico approach

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