Molecular profiling and antimicrobial potential of endophyticGliomastix polychromaCLB32 inhabitingCombretum latifoliumBlume

Abstract

Fungal endophytes as a source of bioactive metabolites have led to the development of pharmaceutical products finding new applications. In a survey of endophytic fungal biodiversity, an antimicrobial endophytic strain CLB32 was isolated from the leaf of Combretum latifolium Blume (Combretaceae) from the Western Ghats of Southern India. CLB32 was then identified as Gliomastix polychroma (KR704576) by morphological and phylogenetic analysis based on internal transcribed spacer (ITS) nuclear rDNA and intervening 5.8S rRNA gene. CLB32 here constituted the first report on incidence of endophytic fungi from C. latifolium Blume. Ethyl acetate fraction of strain CLB32 was evaluated for antimicrobial activity by disc diffusion assay. Secondary metabolites produced effectively inhibited methicillin-resistant Staphylococcus aureus (18.33 ± 0.33 mm), Pseudomonas aeruginosa (14.66 ± 0.33 mm) and Candida albicans (14.00 ± 0.57 mm). Biosynthesis of these antimicrobial compounds was detected by analytical TLC-bioautography method as depicted by zone of inhibition on intensive the band. These findings suggest that G. polychroma CLB32, as a producer of natural antimicrobial drugs, could help to combat against multidrug-resistant infections and also provide baseline information for industrial applications.

Chemogenomics driven discovery of endogenous polyketide anti-infective compounds from endosymbiotic Emericella variecolor CLB38 and their RNA secondary structure analysis

“…Similarly, endophytic actinomycetes and endophytic fungi inhabiting C . latifolium Blume which collected from the Western Ghats of India, were also reported to show antimicrobial activity in our previous studies [ 55 , 56 ]. Hence, this study expands our knowledge of potent hidden endosymbionts from underexplored area are a potential source of novel antimicrobial agents.…”

“…ESI-TOF-MS analysis of compound 1 exhibited a molecular ion peak m/z 300.0458 corresponding to the mass of evariquinone [ 17 ] whereas compound 2 exhibited a molecular ion peak m/z 210.0559 corresponding to the mass of emerimidine A. Literature survey of TOF-MS spectral data with that of emerimidine A indicates that, the metabolite could be a derivative of isoindolones [ 56 ]. TOF-MS is a powerful tool for accurate and rapid identification of known compounds, i.e.…”

Molecular profiling and antioxidant as well as anti-bacterial potential of polyphenol producing endophytic fungus-Aspergillus austroafricanus CGJ-B3

“…Isolation of potent bioactive compounds from the endophytic fungi is in great demand as it has several beneficial applications in pharmaceutical, agricultural and pharmaceutical industries. Endophytic fungal extracts have been reported to possess antimicrobial, anticancer, antimalarial (Wiyakrutta et al 2004;Santos et al 2012), antitumor (Li et al 2005), antidiabetic (Ushasri & Anusha 2015), antimicrobial (Supaphon et al 2013;Hussain et al 2014;Shen et al 2014;Rao et al 2015) and antioxidant activity (Liu et al 2007;Yadav et al 2014). Many endophytes have been isolated from Z. officinale and these endophytes have been reported to possess antimicrobial (Ginting et al 2013), antioxidant activity (Bussaban et al 2003).…”

Endophytism and bioactivity of endophytic fungi isolated from Combretum lanceolatum Pohl ex Eichler

“…Regarding the genus Combretum, Rao et al (2015) reported the activity of the fungus Gliomastix polychroma, isolated from the leaves of C. latifolium, secondary metabolites produced by the fungus inhibited the human pathogens S. aureus, P. aeruginosa and Candida albicans, and biosynthesis of these antimicrobial compounds was detected by analytical TLC-bioautography method.…”