Chromatography of chloroplast carotenoids on magnesium oxide thin layers

Sadowski, R.; Wójcik, Wiesław

doi:10.1016/s0021-9673(01)88140-1

Cited by 15 publications

(3 citation statements)

References 23 publications

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“…Relatively little research has been aimed at the analysis of isomeric carotenoids. Isomers of a-and /3-carotene have been analyzed by chromatography on supports of calcium hydroxide, magnesium oxide, or calcium hydroxide-magnesium hydroxide mixtures (Bickoff et al, 1949; Panalaks and Murray, 1970;Sweeney and Marsh, 1970;Sadowski and Wojcik, 1983). We have observed that the published techniques required for separation of isomers are cumbersome and results are difficult to reproduce.…”

Section: Introductionmentioning

confidence: 99%

Detection of cis-trans carotene isomers by two-dimensional thin-layer and high-performance liquid chromatography

Schwartz¹,

Patroni-Killam²

1985

J. Agric. Food Chem.

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High-performance liquid chromatography (HPLC) was used to detect cis-trans isomers of a-and /3-carotene. Monitoring HPLC eluants at both 436 and 340 nm and calculating the peak height ratio, 340 nm/436 nm, permit detection of isomers in carotene-petroleum ether solutions and plant extracts. Separation of individual isomers was achieved on thin-layer chromatography (TLC) plates prepared with calcium hydroxide layers. Equilibration of the plates to 44% relative humidity prior to development with 1.5% p-methylanisole in petroleum ether was necessary to achieve optimal resolution. No isomeric carotenoids were found in the fresh yellow-orange vegetables, but analyses of chlorophyll-containing vegetables indicated that isomers were present. Heat-processed products contained a variety of cis isomers. Sample handling, saponification methods, and sample pH did not influence the formation of isomers.

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Section: Introductionmentioning

confidence: 99%

Detection of cis-trans carotene isomers by two-dimensional thin-layer and high-performance liquid chromatography

Schwartz¹,

Patroni-Killam²

1985

J. Agric. Food Chem.

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“…This difference between MgO and commercial silica may be attributed to the following facts: the interactions between MgO and analytes enhance with the numbers of aromatic rings in the analyte structures increasing [46]. As a consequence, lipids (flax oil and DIBP) and sulphur can easily be rinsed out from MgO column, owing to lack of enough aromatic rings in their structures [41,47]. On the contrary, lipids retained strongly on silica-based materials, due to the presence of Van der Waals, dipole-dipole and other forces.…”

Section: Mgo Microspheres Silica Gelmentioning

confidence: 99%

The selective cleanup of complex matrices and simultaneous separation of benzo[a]pyrene by solid-phase extraction with MgO microspheres as sorbents

Jin

Zhang

et al. 2011

Journal of Chromatography A

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“…These systems can also be used in the investigation of animal pigments. MgO layers should be employed for the separation of isomeric carotenoids (42), although alumina layers have also been used for this purpose (43).…”

Section: B Tlcmentioning

confidence: 99%