Chromatographic study of PAH-βCD inclusion complexes using a binary mixture and cyano-stationary phase

Abstract

Key WordsColumn liquid chromatography 13-cyclodextrin polycychc aromatic hydrocarbons cyano-stationary phase complex adsorption SummaryThe retention mechanism of a series of five polycyclic aromatic hydrocarbons (PAHs) in highperformance liquid chromatography (HPLC) was investigated using a cyano-bonded silica column and 13-cyclodextrin as mobile phase additive (0 -7.5 mM). Two different models were proposed to investigate the retention mechanism. The first approach considered the interaction of the CD-PAH complex with the stationary phase negligible (i); the second reflected on this interaction (ii). It appeared that model (ii) described more accurately the retention behavior of the five PAHs in this chromatographic system. The stoichiometry of the 13-CD-PAHs inclusion complexes was found to be 1:1 for fluorene, anthracene, phenanthrene and fluoranthene and 2:1 for pyrene. In addition, the association constants were calculated and are discussed in relation to the PAH structures.

Cyclodextrin-grafted electrospun cellulose acetate nanofibers via “Click” reaction for removal of phenanthrene

“…However, the low-water solubility of these components limits the remediation process of contaminated water and soil [39,40]. As it is known, CDs are capable of encapsulating organic compounds due to their hydrophobic cavity and there are many studies reported complexation between CDs and PAHs molecules [51][52][53][54][55]. Phenanthrene is the most commonly known example through other hydrocarbons, so in this study, it was chosen as a model PAH to examine the molecular filtration potential of CA and CA-CD nanofibers.…”

Enantioseparation of five racemic N‐alkyl drugs by reverse phase HPLC using sulfobutylether‐β‐cyclodextrin as a chiral mobile phase additive

“…As shown in Figures S2 and S3, linear relationships of $$\frac{1}{k}$$ versus $${[ {CD} ]_m}$$ and $$\frac{{{{[ {CD} ]}_m}^x}}{{{t_0}{\rm{^{\prime}}} - {t_G}}}$$ versus $${[ {CD} ]_m}^x$$ were observed within the concentrations of SBE‐β‐CD ranged from 0 to 10 mM for racemates of the five drugs, reflecting that the inclusion complex was formed by enantiomers and SBE‐β‐CD with 1:1 stoichiometry. It was reported that the accuracy of the fitting curves was better when F value was used to assess the established model rather than R value [20].…”

Spectroscopic characterization of both aqueous and solid-state diacerhein/hydroxypropyl-β-cyclodextrin inclusion complexes

“…The retention factors of DARh in the experimental condition adopted were weakly decreased with increasing concentration of HPβCD, according to the coexistence of two physicochemical processes: the solute complexation by HPβCD, and the transfer of uncomplexed solute from the hydro-organic phase to the stationary phase. In these conditions, the interaction of the DARh/HPβCD with the stationary phase can be considered as negligible [22], so it is not taken into account and the retention factors, k, for DARh can be derived from Equation 3. The linear relationship between 1/k and HPβCD concentration, with correlation coefficients higher than 0.988 (data not shown), indicates that the behaviour of these equilibria is well described by the model assuming a 1:1 stoichiometry of host-guest interactions.…”