Chromatographic Methods for Quantitative Determination of Ampicillin, Dicloxacillin and Their Impurity 6-Aminopenicillanic Acid

Abdelrahman, Maha M.; Naguib, Ibrahim A.; Elsayed, Mohamed A.; Zaazaa, Hala E.

doi:10.1093/chromsci/bmx101

Cited by 11 publications

(8 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…More intriguingly, AMOX and DIC were detected in their binary mixture using spectrophotometric methods [28][29][30][31][32] and chromatographic methods [33][34][35]. Ternary mixture of DIC, ampicillin, and 6-APA was analyzed by HPTLC [36]. Additionally, DIC, AMOX, and many antibacterial agents were analyzed in poultry feathers by UPLC method [37] However, the reported methods offered lower efficiency towards the separation of AMOX and DIC in the presence of 6-APA either in pure form or in the formulation.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of Ecofriendly HPLC-MS Method for Quantitative Assay of Amoxicillin, Dicloxacillin, and Their Official Impurity in Pure and Dosage Forms

Almalki

Hussein

Naguib

et al. 2021

Journal of Analytical Methods in Chemistry

View full text Add to dashboard Cite

Novel, accurate, selective, and rapid high-performance liquid chromatography mass spectrometry method was developed for simultaneous analysis of amoxicillin trihydrate, dicloxacillin sodium, and their official impurity 6-aminopenicillanic acid. The chromatographic separation was carried out by applying the mixture on a C18 column (3.5 µm ps, 100 mm × 4.6 mm id) using acetonitrile:water (65 : 35 by volume) as a mobile phase within only 4 min. The quantitative analysis was executed using single quadrupole mass spectrometer in which electrospray ionization, selected ion monitoring, and negative mode were operated. The retention times were 1.61, 2.54, and 3.50 mins for amoxicillin, 6-aminopenicillanic acid, and dicloxacillin, respectively. The method was validated in linear ranges of 2–28 µg mL−1, 2–35 µg mL−1, and 1–10 µg mL−1 for amoxicillin, dicloxacillin, and 6-aminopenicillanic acid, respectively. The results obtained from the suggested HPLC/MS were statistically compared with those obtained from the reported HPLC method, where no significant difference appeared respecting accuracy and precision. According to the analytical eco-scale assessment method, the proposed method was proved to be greener than the reported one, where the analysis time and the amount of the wasted effluent decreased.

show abstract

Section: Introductionmentioning

confidence: 99%

Development and Validation of Ecofriendly HPLC-MS Method for Quantitative Assay of Amoxicillin, Dicloxacillin, and Their Official Impurity in Pure and Dosage Forms

Almalki

Hussein

Naguib

et al. 2021

Journal of Analytical Methods in Chemistry

View full text Add to dashboard Cite

show abstract

“…The methods used in this experiment were developed through accessing other relevant stability-indicating studies available in the literature. [10][11][12] The final HPLC-UV method was validated for system suitability, linearity, accuracy, precision, and specificity. To evaluate system suitability, theoretical plates, tailing factor, and peak resolution were calculated using the ampicillin peak area and LCMS solutions software (v 3.0; Kyoto, Japan).…”

Section: Validation Of Stability-indicating Hplc Methodsmentioning

confidence: 99%

Stability of Ampicillin in Normal Saline Following Refrigerated Storage and 24-Hour Pump Recirculation

2020

View full text Add to dashboard Cite

Objective: Use of ampicillin in outpatient parenteral antimicrobial therapy (OPAT) has historically been complicated by frequent dosing and limited stability. The purpose of this study was to evaluate stability of ampicillin using high-pressure liquid chromatography (HPLC) in an OPAT dosing model using continuous infusion at room temperature over 24 hours immediately following preparation compared with batches stored under refrigeration for 24 hours, 72 hours, and 7 days. Methods: An HPLC method was developed and validated as stability indicating using guidance in USP general Chapter <1225>. Four ampicillin batches were prepared for each experimental condition (immediate use and refrigerated storage for 24 hours, 72 hours, and 7 days). A pump was used to recirculate the solutions through medical-grade tubing for 24 hours. Triplicate 1-mL aliquots were removed from each batch at time 0, 4, 8, 12, and 24 hours and analyzed for ampicillin concentration. Results: Each batch was assayed for initial concentration (20.34-21.50 mg/mL), and percent recovery compared with that concentration thereafter. For the duration of infusion, the average recoveries were 96.4%, 95.8%, 94.6%, and 90.3% for immediate use, 24-hour storage, 72-hour storage, and 7-day storage, respectively. The recovery remained above 90% for all batches and time points, except for 7-day storage, which fell below 90% after 4 hours of circulation. Conclusion: Ampicillin can be prepared and stored in a refrigerator for up to 72 hours prior to continuously infusing at room temperature over 24 hours with less than a 10% loss of potency over the dosing period. This model supports twice weekly OPAT delivery of ampicillin.

show abstract

“…The evaluation is based on the SD of the response and the slope of the curves of the four studied pesticides, but using a visual non instrumental method regarding to ICH recommendations [15][16][17][18][19][20][21][22][23] for HPTLC method (Table 1).…”

Section: Limits Of Detection and Quantizationmentioning

confidence: 99%

Two chromatographic methods for the quantitative determination of some pesticides applied for cucumber pests in Egypt

Abdelfatah

Hegazy

Mahmoud

et al. 2018

Separation Science Plus

View full text Add to dashboard Cite

Two novel sensitive and accurate thin‐layer chromatography and high‐performance liquid chromatography methods were used for monitoring the residues of four pesticides: mandipropamid, metalaxyl, thiophenate methyl and carbendazim, which are widely used to eliminate cucumber pests in Egypt. The first method is a thin‐layer chromatography method, using silica gel plates as a stationary phase and hexane/methanol/acetone/glacial acetic acid (7.5:2:0.5:0.1, v/v) as a developing system. The second method is high‐performance liquid chromatography using isocratic elution and a mobile phase consisting of acetonitrile/0.02 molar potassium dihydrogen phosphate buffer (25:75, v/v) at a flow rate of 1 mL min−1 with UV detection at 225 nm. All the obtained results were evaluated regarding the agricultural committee of Egypt and showed acceptable pesticides residues.

show abstract

Chromatographic Methods for Quantitative Determination of Ampicillin, Dicloxacillin and Their Impurity 6-Aminopenicillanic Acid

Cited by 11 publications

References 13 publications

Development and Validation of Ecofriendly HPLC-MS Method for Quantitative Assay of Amoxicillin, Dicloxacillin, and Their Official Impurity in Pure and Dosage Forms

Development and Validation of Ecofriendly HPLC-MS Method for Quantitative Assay of Amoxicillin, Dicloxacillin, and Their Official Impurity in Pure and Dosage Forms

Stability of Ampicillin in Normal Saline Following Refrigerated Storage and 24-Hour Pump Recirculation

Two chromatographic methods for the quantitative determination of some pesticides applied for cucumber pests in Egypt

Contact Info

Product

Resources

About