Chromatographic determination of 6-substituted 2-thiouracyls, thyreostatic preparations

Chernov’yants, M. S.; Dolinkin, A. O.; Braslavskaya, I. V.

doi:10.1134/s1061934808090086

Cited by 4 publications

(2 citation statements)

References 14 publications

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“…The authors of [53] selected the optimum conditions for the separation and assay (borate buffer pH 9.18, voltage 20 kV) of antithyroid agents, i.e., 6-R-2-thiouracils, where R = H (TU), CH 3 (MTU), or n-C 3 H 7 (PTU), by capillary zonal electrophoresis in a quartz capillary of size 60 cm x 75 mm. This method allowed substances to be estimated over the concentration ranges 1.3 -103, 1.4 -114, and 1.7 -136 mg/ml, with detection limits of 0.93, 0.73, and 0.86 mg/ml for TU, MTU, and PTU respectively.…”

Section: Chromatographic Analysis Methodsmentioning

confidence: 99%

Analysis of thyrostatic heteroaromatic thioamides (review)

Dolinkin

Chernov’yants

2010

Pharm Chem J

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This review discusses methods widely used in pharmaceutical and clinical practice for the identification and assay of the thyrostatic agents imidazoline-2-thione and uracil-2-thiones. Sample preparation stages (solid-phase and liquid extraction from biological materials) are discussed, along with chemical modification of drug molecules. A scheme for a hypothetical model of the inhibitory action of thyrostatic agents on thyroid hormone biosynthesis is presented. The analytical characteristics of individual methods for assaying thyrostatic agents discussed in this review can be used in manufacturing at the quality control stage.

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Section: Chromatographic Analysis Methodsmentioning

confidence: 99%

Analysis of thyrostatic heteroaromatic thioamides (review)

Dolinkin

Chernov’yants

2010

Pharm Chem J

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“…Early on, the concentration of thyreostatic residues was determined with thin‐layer chromatography (TLC) (De‐Brabander and Verbeke 1984; Moretti and others 1988), gas chromatography (GC) (Zhang and others 2005; Liu and others 2007; Chernovyants and others 2008), and liquid chromatography (LC) (Asea and others 2006; Abuin and others 2008). It should be mentioned that although chromatographic techniques provide accurate results, they may be unsuitable for the analysis of large sample sets, because the separation step is time‐consuming.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Spectrophotometric Determination of 2‐Thiouracil and 2‐Mercaptobenzimidazole in Animal Tissue Using Multivariate Calibration Methods: Concerns and Rapid Methods for Detection

Beheshti¹,

Riahi²,

Pourbasheer³

et al. 2010

Journal of Food Science

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Two multivariate calibration methods, partial least squares (PLS) and principal component regression (PCR), were applied to the spectrophotometric simultaneous determination of 2-mercaptobenzimidazole (MB) and 2-thiouracil (TU). A genetic algorithm (GA) using partial least squares was successfully utilized as a variable selection method. The concentration model was based on the absorption spectra in the range of 200 to 350 nm for 25 different mixtures of MB and TU. The calibration curve was linear across the concentration range of 1 to 10 microg mL(-1) and 1.5 to 15 microg mL(-1) for MB and TU, respectively. The values of the root mean squares error of prediction (RMSEP) were 0.3984, 0.1066, and 0.0713 for MB and 0.2010, 0.1667, and 0.1115 for TU, which were obtained using PCR, PLS, and GA-PLS, respectively. Finally, the practical applicability of the GA-PLS method was effectively evaluated by the concurrent detection of both analytes in animal tissues. It should also be mentioned that the proposed method is a simple and rapid way that requires no preliminary separation steps and can be used easily for the analysis of these compounds, especially in quality control laboratories.

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