Simultaneous Spectrophotometric Determination of 2‐Thiouracil and 2‐Mercaptobenzimidazole in Animal Tissue Using Multivariate Calibration Methods: Concerns and Rapid Methods for Detection

Abstract

Two multivariate calibration methods, partial least squares (PLS) and principal component regression (PCR), were applied to the spectrophotometric simultaneous determination of 2-mercaptobenzimidazole (MB) and 2-thiouracil (TU). A genetic algorithm (GA) using partial least squares was successfully utilized as a variable selection method. The concentration model was based on the absorption spectra in the range of 200 to 350 nm for 25 different mixtures of MB and TU. The calibration curve was linear across the concentration range of 1 to 10 microg mL(-1) and 1.5 to 15 microg mL(-1) for MB and TU, respectively. The values of the root mean squares error of prediction (RMSEP) were 0.3984, 0.1066, and 0.0713 for MB and 0.2010, 0.1667, and 0.1115 for TU, which were obtained using PCR, PLS, and GA-PLS, respectively. Finally, the practical applicability of the GA-PLS method was effectively evaluated by the concurrent detection of both analytes in animal tissues. It should also be mentioned that the proposed method is a simple and rapid way that requires no preliminary separation steps and can be used easily for the analysis of these compounds, especially in quality control laboratories.

Preconcentration and determination of 2‐mercaptobenzimidazole by dispersive liquid–liquid microextraction and experimental design

“…As can be seen in Supporting Information Table S1 , the good statistical results are reported, which shows the efficiency of this method in comparison to other methods. Hence, the proposed method was successful in preconcentration and determination of 2‐MBI in real samples.…”

“…A number of methods, including spectrophotometry , GC–MS , HPLC , HPLC–MS/MS , and electrochemical impedance spectroscopy , have been reported for the analysis of 2‐MBI. Among these methods, spectrophotometry uses a relatively inexpensive and simple handling instrument, which is accessible in most laboratories .…”

Development of Electrochemical DNA Sensor Based on Poly (Bromocresol purple) Modified Glassy Carbon Electrode for the Determination of 2-Thiouracil

“…Bu nedenle, 2-TU'yu belirlemek için hızlı ve duyarlı bir yöntemin geliştirilmesi farmakokinetik ve klinik çalışmalar için oldukça önemlidir. Diğer urasil türevleri gibi 2-TU'nun tayini için de spektrofotometrik, kemilüminesans, kütle spektrometri, yüzeyde güçlendirilmiş Raman spektroskopisi ve elektrokimyasal analiz gibi yöntemler kullanılmaktadır [5][6][7][8][9][10][11]. Elektrokimyasal yöntemler, farklı potansiyellerde voltametrik cevap gösteren analit türlerinin araştırılmasında kullanılan yararlı yöntemlerdir.…”

Simultaneous spectrophotometric determination of ceftazidime and sulbactam using multivariate calibration methods

“…25,26 Genetic Algorithm (GA) is a useful method with variable selection problems. 27 This method of numerical optimization that simulate biological evolution based on the Darwin theory. 28 GA was the prediction ability of the model, specically for PLS models.…”

“…28 GA was the prediction ability of the model, specically for PLS models. 27 This method is very complex and the mathematical properties are unknown. 29,30 The algorithm used in this article is an evolution of the algorithm described by Leardi and Gonzalez.…”