Chromatographic and allied methods of analysis for selected mycotoxins

Sydenham, Eric W.; Shephard, Gordon S.

doi:10.1007/978-1-4613-1117-1_3

Cited by 24 publications

(18 citation statements)

References 206 publications

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“…Among these are the electrochemical methods, which base their performance on electrochemical and optical principles, such as thin layer chromatography (TLC), high performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) (Richard, Bennett, Ross, & Nelson, 1993;Lindenmeier, Schieberle, & Rychlik, 2004). The aforementioned methods require extensive clean up of samples, well equipped laboratories, trained personnel, and several hours to complete, in addition to their high cost and use of harmful solvents (Sydenham & Shephard, 1996).…”

Section: Introductionmentioning

confidence: 99%

Rapid determination of total aflatoxins and ochratoxins A in meat products by immuno-affinity fluorimetry

Abd-Elghany

Sallam

2015

Food Chemistry

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Section: Introductionmentioning

confidence: 99%

Rapid determination of total aflatoxins and ochratoxins A in meat products by immuno-affinity fluorimetry

Abd-Elghany

Sallam

2015

Food Chemistry

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“…However, these methods require extensive clean-up of sample extracts, trained staff, use of harmful solvents, in addition to being time-consuming and costly (Sydenham and Shephard 1996;Pittet and Royer 2002;Buttinger et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Reliable indirect competitive ELISA used for a survey of ochratoxin A in green coffee from the North of Paraná State, Brazil

Fujii

Ribeiro

Scholz

et al. 2006

Food Additives and Contaminants

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The performance of an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) based on a monoclonal antibody (mAb) for ochratoxin A (OTA) detection was evaluated in a comparative study with high-performance liquid chromatography (HPLC) analysis using 68 freshly harvested coffee samples from the North of Paraná State, Brazil. The anti-OTA mAb showed high specificity and low cross-reactivity with OTA analogues (OTB and OTalpha), but cross-reacted with OTC. This ic-ELISA showed a detection limit of 3.75 ngg-1 sample, when compared to 0.80 ngg-1 by HPLC, with an ic-ELISA/HPLC correlation coefficient of 0.90. As regards OTA analysis of these coffee samples, natural contamination was detected in 10 samples (14.7%) by both methods, where the ic-ELISA values (range 3.9-7.3 ngg-1) were 1.1 to 1.6-fold higher than HPLC data (2.7-4.7 ngg-1). Five samples (7.4%) were OTA positive (range 0.84-1.30 ngg-1) only by HPLC assay, probably due to the higher detection limit reached by ic-ELISA. OTA was undetectable in 53 samples (77.9%) by both methods, while all positive samples (range 0.84-7.30 ngg-1) showed OTA levels lower than 8 ngg-1 (maximum limit recommended by the European Union). The matrix interference of green coffee was minimized by dilution of sample extracts before carrying out the ELISA assay. This mAb-based ic-ELISA can be effectively applied for OTA screening in coffee, because it is simple, sensitive and sample preparation is easy.

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“…O método oficial descrito em "Association of Official Analytical Chemists" (AOAC) para análise quantitativa de micotoxinas é a Cromatografia Líquida de Alta Eficiência (CLAE). No entanto, a demanda considerável de reagentes de alto grau de pureza, equipamento/manutenção de alto custo, tempo de análise significativo e supervisão por técnicos especializados, restringe o emprego na rotina laboratorial (SYDENHAM; SHEPHARD, 1996;FURLANI;OLIVEIRA;SOARES, 1999). A espectrofluorimetria destaca-se por oferecer simplicidade, sensibilidade, baixo custo, uso reduzido de solventes tóxicos e a ausência de contato do aparelho com estes solventes constituindo uma alternativa promissora para análise de micotoxinas em alimentos.…”

Section: Introductionunclassified

Espectrofluorimetria comparada à cromatografia líquida de alta eficiência na detecção de ocratoxina A em café

et al. 2008

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ResumoA cafeicultura brasileira apresenta grande importância ao mercado interno e comércio exterior. A qualidade e sanidade do café dependem da contaminação fúngica, com ênfase as espécies produtoras de ocratoxina A (OTA), uma nefrotoxina de efeito deletério à saúde pública. A necessidade de contínuo monitoramento e controle de OTA em produtos agrícolas requer metodologia analítica simples, rápida e acessível. O trabalho teve como objetivo padronizar um método espectrofluorimétrico para detecção de OTA visando monitoramento em grãos de café, correlacionando-o com a cromatografia líquida de alta eficiência (CLAE). A aplicabilidade da espectrofluorimetria foi avaliada em café artificialmente contaminado com OTA (10, 20 e 50 ng/g), comparando os resultados obtidos com a CLAE. Embora a fluorimetria tenha apresentado resultados insatisfatórios, com recuperação de 40,14 % e coeficiente de correlação de 0,79 em relação a CLAE, i.e. baixa confiabilidade e eficiência inadequada, a técnica constitui perspectiva para prosseguir o estudo inserindo novos ensaios referentes à sua padronização e certificação. Palavras-chave: Ocratoxina A, café, CLAE, espectrofluorimetria AbstractThe Brazilian coffee production plays important role both in the national and oversea trade. The quality and sanity of coffee depend on fungal contamination, with emphasis on ochratoxin A (OTA) producing species, which is a nephrotoxin with hazardous effect in public health. The exigency on continuous monitoring and control of OTA in agricultural commodity requires a simple, rapid and accessible analytical methodology. The objective of this research was to set up the spectrofluorimetric method for OTA detection intended for monitoring in coffee, which was developed in data-correlation with high efficiency liquid chromatography (HPLC). The performance of spectrofluorimetric analysis was evaluated using artificially contaminated coffee (10, 20 and 50 ng/g), and the data compared with HPLC. Although of the fault of former technique, with recovery of 40.14 % and correlation coefficient of 0.79 when compared to HPLC, i.e. low reliability and inadequate efficiency, the data showed perspective to follow the improvement inserting new tool, and further standardization and certification.

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Chromatographic and allied methods of analysis for selected mycotoxins

Cited by 24 publications

References 206 publications

Rapid determination of total aflatoxins and ochratoxins A in meat products by immuno-affinity fluorimetry

Rapid determination of total aflatoxins and ochratoxins A in meat products by immuno-affinity fluorimetry

Reliable indirect competitive ELISA used for a survey of ochratoxin A in green coffee from the North of Paraná State, Brazil

Espectrofluorimetria comparada à cromatografia líquida de alta eficiência na detecção de ocratoxina A em café

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